CHANGES IN THE CONTENT OF OXIDATIVELY MODIFIED PROTEINS IN FEMALE RATS WITH OVARY PATHOLOGY AND THEIR CORRECTION BY INOSITOL PREPARATION
DOI:
https://doi.org/10.11603/1811-2471.2023.v.i3.14092Keywords:
oxidatively modified proteins, ovaries, blood, age, hypodynamic stress, lead, nanodoses, inositol, FT 500 plusAbstract
SUMMARY. The problem of infertility is quite relevant today. Among the factors of its development are: age; stress, sedentary lifestyle, toxic effects of heavy metal salts. At the same time, there is destruction of cell membranes, which are composed of proteins and phospholipids. The use of inositol-containing preparations promotes the restoration of proteins, which improves reproductive function.
The aim – to evaluate the development of carbonyl stress in female rats with ovarian pathology and the correction made with inositol preparations (In).
Material and Methods. Experiments were performed on 144 white female Wistar rats, which were divided into 4 series: 1 – control, 3.5–4 months old rats, 2 – rats 7.5–8 months old, 3 – rats that were intragastrically injected with acetate lead solution for 15 days in a dose of 0.05 mg/kg, 4 – hypodynamic stress. There were 6 groups of animals in each series: A – control (C), B – In, C – FT 500 plus (FT), D – pregnancy, E – pregnancy + In, F – pregnancy + FT. In and FT were introduced intragastrically for 15 days at the rate of 400 mg/kg of In. Oxidatively modified proteins were determined in blood serum and ovaries at wavelengths of 370, 430, and 530 nm.
Results. In serum of C rats In caused an increase in OMP370 and OMP430, and FT – a decrease in OMP430. In 2A, 3A, 4A groups, compared to 1A, all OMPs increased. In 2B, 3B, 4B and 2C, 3C, 4C, compared to 2A, 3A and 4A, all OMPs decreased, but they did not reach 1B and 1C.
When examining the ovaries of animals of series 1, which were injected with In, compared to C, hyperemia was noted. In and FT caused an increase in OMP370 and OMP430, a decrease in OMP530, and FT – a decrease in OMP430 in serum. In 2A, 3A and 4A, compared to 1A, all OMPs increased. In 2B, 3B, 4B and 2C, 3C, 4C, compared to 2A, 3A and 4A, all OMPs decreased, but they did not reach the values of 1B and 1C rats.
In C parturient females, which were administered In and FT before pregnancy, OMP in blood serum was significantly lower. In 2D, 4D, compared to 1D, all OMPs increased, in 3D – OMP370. In 2E, 4E and 2F, 3F, 4F, compared to 2D, 3D and 4D, all OMPs decreased, and in 3E – OMP370.
In C parturient females, which were administered with In and FT before pregnancy, OMP in the ovaries was significantly lower. In 2D, compared to 1D, ОМP370 and ОМP430 increased, in 3D and 4D – all ОМP. In 2E, 4E and 2F, 3F, 4F, compared to 2D, 3D and 4D, all OMPs decreased, and in 3E – OMP370 and OMP430.
Conclusion. Inositol at a dose of 400 mg/kg should not be used in an experiment in healthy young female rats. In 7.5-8 month-old females, in animals exposed to nanodoses of lead acetate, hypodynamic stress, both inositol and FT 500 plus are optimal for reducing protein breakdown, although the latter is more effective. The effectiveness of both inositol and FT 500 plus when used before pregnancy is high, which is manifested by a lower accumulation of oxidatively modified proteins both in the blood serum and in the ovaries of rats after delivery.
References
Doody, K.J. (2021) Infertility treatment now and in the future. Obstet. Gynecol. Clin. North Am., 48(4), 801-812.
Yuzko, O.M. (2016). Dopomizhni reproduktyvni tekhnolohiyi Ukrayiny – 25 rokiv uspikhu [Assisted reproductive tech-nologies of Ukraine – 25 years of success]. Zb. nauk. pr. Asotsiatsiyi akusheriv-hinekolohiv Ukrayiny – Coll. of science pr. of the Association of Obstetricians and Gynecologists of Ukraine. Kyiv: Yuston [in Ukrainian].
Ancheva, I.A. (2021). Patsiyentky starshoho viku. Piznya vahitnist i materynstvo. [Older patients. Late preg-nancy and motherhood]. Zdorovya Ukrayiny – Health of Ukraine, 3, 10 [in Ukrainian].
Kortekaas, J.C., Kazemier, B.M., Keulen, J.K., Bruinsma, A., Mol, B.W., Vandenbussche, F., ... & De Miranda, E. (2020). Risk of adverse pregnancy outcomes of late-and postterm pregnancies in advanced maternal age: A national cohort study. Acta Obstetricia et Gynecologica Scandinavica, 99(8), 1022-1030.
Valsamakis, G., Chrousos, G., & Mastorakos, G. (2019). Stress, female reproduction and pregnancy. Psychoneuroendocrinology, 100, 48-57.
Rzymski, P., Tomczyk, K., Poniedzialek, B., Opala, T., & Wilczak, M. (2015). Impact of heavy metals on the female reproductive system. Annals of Agricultural and Environmental Medicine, 22(2).
Balabanič, D., Rupnik, M., & Klemenčič, A.K. (2011). Negative impact of endocrine-disrupting com-pounds on human reproductive health. Reproduction, Fertility and Development, 23(3), 403-416.
Qu, J., Niu, H., Wang, J., Wang, Q., & Li, Y. (2021). Potential mechanism of lead poisoning to the growth and development of ovarian follicle. Toxicology, 457, 152810.
Stravskyi, Y.S., & Sergeyev, V.I. (2018). Dezintoksykatsiya orhanizmu koriv u pislyarodovyy period [Detoxification of the body of cows in the postpartum period]. Veterynarna biotekhnolohiya – Veterinary Biotechnology, 32, 515-521 [in Ukrainian].
Yuzko, O.M. (2021). Reproduktyvne zdorovya batkiv: ohlyad literatury [Reproductive health of pa-rents: Review of the literature]. Reproduktyvna endokrynolohiya – Reproductive Endocrinology, 60, 72-76 [in Ukrainian].
Kulik, I.I., & Khmil, S.V. (2020). Vplyv skleroterapyi ta prehravidarnoyi pidhotovky inozytolom i vitaminom D3 na rozmir ta kilkist kist u zhinok z bezpliddyam na foni endometriozu [The effect of sclerotherapy and pre-pregnancy training with inositol and vitamin D3 on the size and number of cysts in women with infertility on the background of endometriosis]. Visn. Vinnyts. nats. med. un-tu – Reports of Vinnytsia National Medical University, 24(3), 444-448 [in Ukrainian].
Alviggi, C., Cariati, F., Conforti, A., De Rosa, P., Vallone, R., Strina, I., ... & De Placido, G. (2016). The effect of FT500 Plus® on ovarian stimulation in PCOS women. Reproductive Toxicology, 59, 40-44.
Sengupta, P. (2013). The laboratory rat: relating its age with human’s. International Journal of Preventive Medicine, 4(6), 624.
Shatorna, V.F., Harets, V.I., Savenkova, O.O., & Kolosova, I.I. (2013). Doslidzhennya vplyvu nanometaliv na stan reproduktyvnoyi funktsiyi v eksperymenti [Study of the influence of nanometals on the state of reproductive function in an experiment]. Tavrycheskyy med.-byol. vestn. – Tauride Medical and Biological Bulletin, 16(1), 246-250 [in Ukrainian].
Major, V.V., Kolosova, I.I., & Shatorna, V.F. (2016). Doslidzhennya vplyvu kompleksu vazhkykh metaliv (svyntsyu, zaliza, zolota, sribla) na stan reproduktyvnoyi systemy [Study of the influence of a complex of heavy metals (lead, iron, gold, silver) on the state of the reproductive system]. Visn. Vinnyts. nats. med. un-tu – Reports of Vinnytsia National Medical University, 2(20), 341-344 [in Ukrainian].
Bevilacqua, A., Dragotto, J., Giuliani, A., & Bizzarri, M. (2019). Myo-inositol and D-chiro-inositol (40:1) reverse histological and functional features of polycystic ovary syndrome in a mouse model. Journal of Cellular Physiology, 234(6), 9387-9398.
Pat. na korysnu model 99821 Ukrayina, MPK G09B 23/28(2006.01). Sposib modelyuvannya khronichnoho immobilizatsiynoho stresu, pidsylenoho diyeyu hostroho stresu. Denefil О.V., Mitz І.R. № u 2014 14143 ; zayavl. 29.12.14 ; opubl. 25.06.15, Byul. No 12 – Patent No. 99821 IPC: G 09 B 23/28; Modeling method of chronic immobilization stress enhanced by acute stress. Denefil О.V., & Mitz І.R. No. u201414143; zyav. 29.12.2014; opubl. 25.06.2015. bul. No 12 [in Ukrainian].
Vlizlo, V.V., Fedoruk, R.S., Ratych, I.B., Vishchur, O.I., Sharan, M.M., Vudmaska, I.V., ... Martyn, Yu.V. (2012). Laboratorni metody doslidzhen u biolohiyi, tvarynnytstvi ta veterynarniy medytsyni : dovidnyk [Laboratory research methods in biology, animal hus bandry and veterinary medicine: a handbook]. Lviv: Spolom [in Ukrainian].
Meshchishen, I.F. (1998). Metod vyznachennya okysnyuval’noyi modyfikatsiyi bilkiv plazmy (syrovatky) krovi [Method for estimation of oxidative modification of blood plasma (serum) protein]. Bukovynskyy medychnyy visnyk – Bukovinian Medical Herald, 2(1), 156-158 [in Ukrainian].
