SKIN MICROBIOME RESIDENTS OF PATIENTS WITH NON-ALCOHOLIC FATTY LIVER DISEASE AND SEBORRHEIC DERMATITIS DETERMINED BY CULTURE METHOD
DOI:
https://doi.org/10.11603/1811-2471.2023.v.i3.14075Keywords:
non-alcoholic fatty liver disease, diabetes, insulin resistance, seborrheic dermatitis, microbiomeAbstract
SUMMARY. Metabolites synthesized by pathogenic residents of the intestinal microbiota contribute to the deposition of fat in liver cells, increased insulin resistance, oxidative stress, and the level of pro-inflammatory cytokines. No studies currently present the influence of metabolic-associated diseases on the microbiome of the skin, and in particular, patients with seborrheic dermatitis (SD).
The aim – to determine the main residents of the skin microbiome of patients with metabolically-associated non-alcoholic fatty liver disease (NAFLD) and seborrheic dermatitis by culture method.
Material and Methods. Smears were taken from 45 NAFLD +SD patients and 18 volunteers and cultured on Uriselect 4 agar, Sabouraud Glucose Agar with Chloramphenicol, Malassezia Leeming & Notman Agar Modified (MLNA), blood agar 5 %, HiCrome Candida Differential Agar, Yeast Extract Sodium Lactate medium (YELA) with the addition of blood. The CFU of bacteria was counted using Shelkova’s method, the Bürker chamber was used for yeast.
Results. Inflamed seborrheic areas are significantly more often colonized by Corynebacterium spp. (р=0.0236), Enterobacteriaceae spp. (р=0.0258), Proteus spp. (р=0.0236), Enterococcus spp. (p=0.001), and tend to increase the number of Candida albicans (p=0.0784), Klebsiella spp. (p=0.0784), and pathogenic Staphylococcus epidermidis with hemolytic activity (p=0.0795), Propionibacterium acnes with hemolytic activity (p=0.2958) and a decrease in the number of commensal Staphylococcus epidermidis (p=0.0749) and Propionibacterium acnes (p=0.3806). Differences in the number of Malassezia spp. was not significant (р=0.6894). Inflamed skin is colonized by a significantly higher number of S. aureus compared to intact areas of patients with SD (р=0.0673) and healthy individuals (р=0.0463); Staphylococcus epidermidis + hemolytic activity (р=0.004; р=0.001); Malassezia spp. (p=0.019; p=0.003); there is a tendency to increase the number of Staphylococcus epidermidis CFU on healthy skin compared to inflamed skin in patients with seborrheic dermatitis (p=0.0934) and no difference with healthy individuals (p=0.454).
Conclusions. SD patients had a significantly higher number of hemolytically active St. epidermidis (р=0.0018) and P. acnes (р=0.0776), Malassezia spp. (р=0.0215) and significantly less commensals like St. epidermidis (р=0.0017) compared to healthy individuals.
References
Zhang, Z., Wang, Q., Yao, J., Zhou, X., Zhao, J., Zhang, X., Dong, J., & Liao, L. (2020). Chemokine Receptor 5, a Double-Edged Sword in Metabolic Syndrome and Cardiovascular Disease. Frontiers in pharmacology, 11, 146. DOI: 10.3389/fphar.2020,00146. DOI: https://doi.org/10.3389/fphar.2020.00146
Masenga, S.K., Kabwe, L.S., Chakulya, M., & Kirabo, A. (2023). Mechanisms of Oxidative Stress in Metabolic Syndrome. International journal of molecular sciences, 24(9), 7898. DOI: 10.3390/ijms24097898. DOI: https://doi.org/10.3390/ijms24097898
Ferrer, M.D., Reynés, C., Monserrat-Mesquida, M., Quetglas-Llabrés, M., Bouzas, C., García, S., … & Pons, A. (2023). Polyunsaturated and Saturated Oxylipin Plasma Levels Allow Monitoring the Non-Alcoholic Fatty Liver Disease Progression to Severe Stages. Antioxidants (Basel, Switzerland), 12(3), 711. DOI: 10.3390/antiox12030711. DOI: https://doi.org/10.3390/antiox12030711
Akbaş, A., Kılınç, F., Şener, S., & Hayran, Y. (2022). Investigation of the relationship between seborrheic dermatitis and metabolic syndrome parameters. Journal of cosmetic dermatology, 21(11), 6079-6085. DOI: 10.1111/jocd.15121. DOI: https://doi.org/10.1111/jocd.15121
Ikeda, K., Morizane, S., Akagi, T., Hiramatsu-Asano, S., Tachibana, K., Yahagi, A., … & Mukai, T. (2022). Obesity and Dyslipidemia Synergistically Exacerbate Psoriatic Skin Inflammation. International journal of molecular sciences, 23(8), 4312. DOI: 10.3390/ijms23084312. DOI: https://doi.org/10.3390/ijms23084312
Wu, J., Wang, K., Wang, X., Pang, Y., & Jiang, C. (2021). The role of the gut microbiome and its metabolites in metabolic diseases. Protein & cell, 12(5), 360-373. DOI: 10.1007/s13238-020-00814-7. DOI: https://doi.org/10.1007/s13238-020-00814-7
Dobrowolski, P., Prejbisz, A., Kuryłowicz, A., Baska, A., Burchardt, P., Chlebus, K., … Bogdański, P. (2022). Metabolic syndrome – a new definition and management guidelines: A joint position paper by the Polish Society of Hypertension, Polish Society for the Treatment of Obesity, Polish Lipid Association, Polish Association for Study of Liver, Polish Society of Family Medicine, Polish Society of Lifestyle Medicine, Division of Prevention and Epidemiology Polish Cardiac Society, "Club 30" Polish Cardiac Society, and Division of Metabolic and Bariatric Surgery Society of Polish Surgeons. Archives of medical science : AMS, 18(5), 1133-1156. DOI: 10.5114/aoms/152921. DOI: https://doi.org/10.5114/aoms/152921
A new proposed severity score for seborrheic dermatitis of the face: SEborrheic Dermatitis Area and Severity Index (SEDASI). Journal of the American academy of dermatology, 76, 6. DOI: 10.1016/j.jaad.2017.04.088. DOI: https://doi.org/10.1016/j.jaad.2017.04.088
http://www.liofilchem.net/login/pd/ts/610363_TS.pdf.
Shelkova, N., & Prokopets, V. (2009). Quantitative research method of maintenance of bacteria in clinical species which are selected by waddin tampon. Coliection of scientific works of staff member of Shupyk NHU of Ukraine, 17(2), 698-702. DOI: 10.13140/RG.2.1.3161.3049.
Gunetti, M., Castiglia, S., Rustichelli, D., Mareschi, K., Sanavio, F., Muraro, M., … & Fagioli, F. (2012). Validation of analytical methods in GMP: the disposable Fast Read 102® device, an alternative practical approach for cell counting. Journal of translational medicine, 10, 112. DOI: 10.1186/1479-5876-10-112. DOI: https://doi.org/10.1186/1479-5876-10-112
Tanaka, A., Cho, O., Saito, C., Saito, M., Tsuboi, R., & Sugita, T. (2016). Comprehensive pyrosequencing analysis of the bacterial microbiota of the skin of patients with seborrheic dermatitis. Microbiology and immunology, 60(8), 521-526. DOI: 10.1111/1348-0421.12398. DOI: https://doi.org/10.1111/1348-0421.12398
Tao, R., Wang, R., Wan, Z., Song, Y., Wu, Y., & Li, R. (2022). Ketoconazole 2 % cream alters the skin fungal microbiome in seborrhoeic dermatitis: a cohort study. Clinical and experimental dermatology, 47(6), 1088-1096. DOI: 10.1111/ced.15115. DOI: https://doi.org/10.1111/ced.15115
Soares, R.C., Camargo-Penna, P.H., de Moraes, V.C., De Vecchi, R., Clavaud, C., Breton, L., Braz, A.S., & Paulino, L.C. (2016). Dysbiotic Bacterial and Fungal Communities Not Restricted to Clinically Affected Skin Sites in Dandruff. Frontiers in cellular and infection microbiology, 6, 157. DOI: 10.3389/fcimb.2016.00157. DOI: https://doi.org/10.3389/fcimb.2016.00157
Puviani, M., Campione, E., Offidani, A.M., De Grandi, R., Bianchi, L., Bobyr, I., … & Milani, M. (2019). Effects of a cream containing 5 % hyaluronic acid mixed with a bacterial-wall-derived glycoprotein, glycyrretinic acid, piroctone olamine and climbazole on signs, symptoms and skin bacterial microbiota in subjects with seborrheic dermatitis of the face. Clinical, cosmetic and investigational dermatology, 12, 285-293. DOI: 10.2147/CCID.S205904. DOI: https://doi.org/10.2147/CCID.S205904
Ahle, C.M., Stødkilde, K., Poehlein, A., Bömeke, M., Streit, W.R., Wenck, H., … & Brüggemann, H. (2022). Interference and co-existence of staphylococci and Cutibacterium acnes within the healthy human skin microbiome. Communications biology, 5(1), 923. DOI: 10.1038/s42003-022-03897-6. DOI: https://doi.org/10.1038/s42003-022-03897-6
Christensen, G.J., Scholz, C.F., Enghild, J., Rohde, H., Kilian, M., Thürmer, A., Brzuszkiewicz, E., Lomholt, H.B., & Brüggemann, H. (2016). Antagonism between Staphylococcus epidermidis and Propionibacterium acnes and its genomic basis. BMC genomics, 17, 152. DOI: 10.1186/s12864-016-2489-5 DOI: https://doi.org/10.1186/s12864-016-2489-5
Dréno, B., Dagnelie, M. A., Khammari, A., & Corvec, S. (2020). The Skin Microbiome: A New Actor in Inflammatory Acne. American journal of clinical dermatology, 21(1), 18-24. DOI: 10.1007/s40257-020-00531-1. DOI: https://doi.org/10.1007/s40257-020-00531-1
Wang, Y., Kuo, S., Shu, M., Yu, J., Huang, S., Dai, A., Two, A., Gallo, R.L., & Huang, C.M. (2014). Staphylococcus epidermidis in the human skin microbiome mediates fermentation to inhibit the growth of Propionibacterium acnes: implications of probiotics in acne vulgaris. Applied microbiology and biotechnology, 98(1), 411-424. DOI: 10.1007/s00253-013-5394-8. DOI: https://doi.org/10.1007/s00253-013-5394-8
Claudel, J.P., Auffret, N., Leccia, M.T., Poli, F., Corvec, S., & Dréno, B. (2019). Staphylococcus epidermidis: A Potential New Player in the Physiopathology of Acne? Dermatology (Basel, Switzerland), 235(4), 287-294. DOI: 10.1159/000499858 DOI: https://doi.org/10.1159/000499858
Skabytska, Y., & Biedermann, T. (2016). Staphylococcus epidermidis Sets Things Right Again. The Journal of investigative dermatology, 136(3), 559-560. DOI: 10.1016/j.jid.2015.11.016. DOI: https://doi.org/10.1016/j.jid.2015.11.016
Abbott, C., Grout, E., Morris, T., & Brown, H.L. (2022). Cutibacterium acnes biofilm forming clinical isolates modify the formation and structure of Staphylococcus aureus biofilms, increasing their susceptibility to antibiotics. Anaerobe, 76, 102580. DOI: 10.1016/j.anaerobe. 2022.102580. DOI: https://doi.org/10.1016/j.anaerobe.2022.102580
Wang, H.C., Wang, C.S., Hsieh, S.C., Hung, Y.T., & Chen, H.H. (2022). Evaluation of a new-formula shampoo containing 6 % glycyrrhetinic acid complex for scalp seborrheic dermatitis: A pilot study. Journal of cosmetic dermatology, 21(8), 3423-3430. DOI: 10.1111/jocd.14623. DOI: https://doi.org/10.1111/jocd.14623
An, Q., Sun, M., Qi, R. Q., Zhang, L., Zhai, J.L., Hong, Y.X., Song, B., Chen, H.D., & Gao, X.H. (2017). High Staphylococcus epidermidis Colonization and Impaired Permeability Barrier in Facial Seborrheic Dermatitis. Chinese medical journal, 130(14), 1662-1669. DOI: 10.4103/0366-6999.209895. DOI: https://doi.org/10.4103/0366-6999.209895
