SKIN MICROBIOME RESIDENTS OF PATIENTS WITH NON-ALCOHOLIC FATTY LIVER DISEASE AND SEBORRHEIC DERMATITIS DETERMINED BY CULTURE METHOD
DOI:
https://doi.org/10.11603/1811-2471.2023.v.i3.14075Keywords:
non-alcoholic fatty liver disease, diabetes, insulin resistance, seborrheic dermatitis, microbiomeAbstract
SUMMARY. Metabolites synthesized by pathogenic residents of the intestinal microbiota contribute to the deposition of fat in liver cells, increased insulin resistance, oxidative stress, and the level of pro-inflammatory cytokines. No studies currently present the influence of metabolic-associated diseases on the microbiome of the skin, and in particular, patients with seborrheic dermatitis (SD).
The aim – to determine the main residents of the skin microbiome of patients with metabolically-associated non-alcoholic fatty liver disease (NAFLD) and seborrheic dermatitis by culture method.
Material and Methods. Smears were taken from 45 NAFLD +SD patients and 18 volunteers and cultured on Uriselect 4 agar, Sabouraud Glucose Agar with Chloramphenicol, Malassezia Leeming & Notman Agar Modified (MLNA), blood agar 5 %, HiCrome Candida Differential Agar, Yeast Extract Sodium Lactate medium (YELA) with the addition of blood. The CFU of bacteria was counted using Shelkova’s method, the Bürker chamber was used for yeast.
Results. Inflamed seborrheic areas are significantly more often colonized by Corynebacterium spp. (р=0.0236), Enterobacteriaceae spp. (р=0.0258), Proteus spp. (р=0.0236), Enterococcus spp. (p=0.001), and tend to increase the number of Candida albicans (p=0.0784), Klebsiella spp. (p=0.0784), and pathogenic Staphylococcus epidermidis with hemolytic activity (p=0.0795), Propionibacterium acnes with hemolytic activity (p=0.2958) and a decrease in the number of commensal Staphylococcus epidermidis (p=0.0749) and Propionibacterium acnes (p=0.3806). Differences in the number of Malassezia spp. was not significant (р=0.6894). Inflamed skin is colonized by a significantly higher number of S. aureus compared to intact areas of patients with SD (р=0.0673) and healthy individuals (р=0.0463); Staphylococcus epidermidis + hemolytic activity (р=0.004; р=0.001); Malassezia spp. (p=0.019; p=0.003); there is a tendency to increase the number of Staphylococcus epidermidis CFU on healthy skin compared to inflamed skin in patients with seborrheic dermatitis (p=0.0934) and no difference with healthy individuals (p=0.454).
Conclusions. SD patients had a significantly higher number of hemolytically active St. epidermidis (р=0.0018) and P. acnes (р=0.0776), Malassezia spp. (р=0.0215) and significantly less commensals like St. epidermidis (р=0.0017) compared to healthy individuals.
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