HISTOLOGICAL CHANGES IN ANIMAL LIVER, KIDNEY IN THE ADMINISTRATION OF ZINC OXIDE NANOPARTICLES AND HERBICIDE GLYPHOSATE
DOI:
https://doi.org/10.11603/1811-2471.2023.v.i2.13949Keywords:
zinc oxide nanoparticles, glyphosate, liver, kidneys, spleenAbstract
SUMMARY. One of the most promising types of nanomaterials are highly dispersed zinc oxide nanoparticles, which are increasingly used in biomedicine. Despite their widespread use, their safety for humans has not yet been established. A potentially dangerous situation can be when nanoparticles enter the body together with chemical toxins, for example, herbicides.
The aim – to study the integral effect of zinc oxide nanoparticles and glyphosate herbicide on the morphological structure of animal organs.
Material and methods. White rats were administered orally for 14 days with 0.5 ml of a suspension of zinc oxide nanoparticles at a dose of 100 mg/kg of body weight, 0.5 ml of a glyphosate solution at a dose of 250 mg/kg of body weight, or nanoparticles and glyphosate at the same doses. On the 15th day, liver, kidney and spleen tissues were used for morphological studies.
Results. Administration of zinc oxide nanoparticles to experimental animals leads to only minor changes in the vascular and parenchymal components of the liver, kidneys, and spleen. The use of the herbicide glyphosate causes significant vascular disorders (dilation and congestion of veins, spasm of the lumen of interlobular arteries, expansion of sinusoids with thrombosis phenomena, thickening of the media, edema of myocytes, edema of perivascular connective tissue, congestion of hemocapillaries of glomeruli, stasis and sludge in peritubular capillaries) and disorders of the examined organs histoarchitectonics (disorders of the hepatocytes arrangement, loss of karyolemma contours, vacuolar dystrophy of the cytoplasm, hypertrophy of the renal corpuscles, narrowing of the lumen of the capsule, deformation of the renal corpuscles, hydropic dystrophy of the epitheliocytes of the proximal tubules, edema and dystrophy of nephrocytes, increased hydration of the main connective tissue substance, destructuring of the white pulp, active proliferation of lymphocytes). The most pronounced vascular, inflammatory and destructive-degenerative changes in all structural components of the liver, kidneys and spleen are observed in animals that were simultaneously administered with the toxicant glyphosate and zinc oxide nanoparticles.
Conclusion. Zinc oxide nanoparticles potentiate the ability of the herbicide glyphosate to cause morphological changes in the liver, kidneys, and spleen.
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