QUANTITATIVE MORPHOLOGICAL ANALYSIS OF THE FEATURES OF REMODELING OF THE VENOUS BAD OF THE LOWER EXTREMITY AT EXPERIMENTAL WOUNDS
DOI:
https://doi.org/10.11603/1811-2471.2023.v.i1.13722Keywords:
experimental wound, lower extremity, venous badAbstract
SUMMARY. The aim – to investigate the peculiarities of the remodeling of the venous bed of the lower extremity at of experimental wounds in conditions of venous hypertension.
Material and Methods. The venous bad of the lower extremity of 45 white rats, which were divided into 3 groups, was studied. The 1 control group – 15 intact animals, the 2nd – 15 rats with a 10-day simulated wound of the hind limb and venous hypertension, the 3rd – 15 animals with a similar 14-day wound. Animals were euthanized by bloodletting under thiopental anesthesia. The outer and inner diameters of venous vessels, the thickness of their walls, the height of endotheliocytes, the diameter of their nuclei, the nuclear-cytoplasmic ratio in these cells, and the relative volume of damaged endotheliocytes were determined on micropreparations of the lower limb. Quantitative values were processed statistically.
Results. It was established that the simulated pathology led to a pronounced structural rearrangement of the veins of the lower limbs of the experimental animals. An increase in the outer and inner diameters of the veins was found in the simulated pathology. a decrease in the thickness of their walls, a violation of cellular structural homeostasis in endotheliocytes, an increase in the relative volumes of damaged endotheliocytes, congestion of venous vessels, a violation of venous drainage, dystrophic, necrobiotic, infiltrative, and sclerotic processes in the studied limb, which dominated on the 14th day of the experiment.
References
Aksionov, Ye.V. (2019). Endotelialna dysfunktsiya ta shlyakhy yiyi profilaktyky pry provedenni renthenendovaskulyarnykh protsedur po rekanalizatsiyi koronarnykh arteriy [Endothelial dysfunction and ways of its prevention during X-ray endovascular procedures for recanalization of coronary arteries]. Ukrayinskyy zhurnal medytsyny, biolohiyi ta sportu – Ukrainian Journal of Medicine, Biology and Sports, 5(21), 105-108 [in Ukrainian].
Bahrii, M.M., Dibrova, V.A., Popadynets, O.H., & Hryshchuk, M.I. (2016). Metodyky morfolohichnykh doslidzhen [Methods of morphological research]. Vinnytsia: Nova knyha [in Ukrainian].
Baibakov, V.M. (2011). Morfofunktsionalni zminy venoznoho rusla yak lanky drenazhnoi systemy yaiechka pry travmuvanni sudynnykh anastomoziv simianoho kanatyka v eksperymenti [Morphofunctional changes of the venous bed as a link of the drainage system of the testis in case of trauma to the vascular anastomoses of the spermatic cord in the experiment]. Klinichna anatomiia ta operatyvna khirurhiia – Clinucal Anatomy and Operative Surgery, 10 (4), 32-35 [in Ukrainian]. DOI: https://doi.org/10.24061/1727-0847.10.4.2011.7
Hnatiuk, M.S., Bodnarchuk, I.V., & Tatarchuk, L.V. (2019). Osoblyvosti strukturnoyi perebudovy arteriy yazyka pry deskvamatyvnomu hlosyti [Peculiarities of structural rearrangement of the arteries of the tongue in desqu amative glossitis]. Visnyk naukovykh doslidzhen – Bulletin of Scientific Research, 2, 85-89 [in Ukrainian]. DOI: https://doi.org/10.29254/2077-4214-2019-2-1-150-243-245
Hnatiuk, M.S., Hdanska, N. M., Tatarchuk, L.V., & Yasinovskyi, O.B. (2021). Osoblyvosti remodeliuvannia venoznykh sudyn shlunochkiv sertsia pry postrezektsiinii portalnii hipertenzii [Peculiarities of remodeling of venous vessels of heart ventricles in post-resection portal hypertension]. Visnyk problem biolohii i medytsyny – Herald of Problems of Biology and Medicine, 2(160), 206-208 [in Ukrainian].
Grzybowski, А.І., Ivanov, О.В., & Gorbatova, M.A. (2016). Sravneniye kolichestvennykh dannykh dvukh parnykh vyborok s ispolzovaniyem programmnogo obespecheniya Statistica i SPSS: parametricheskiye i neparametricheskiye kriterii [Comparison of quantitative data of two pair samples using Statistica and SPSS software: parametric and nonparametric criteria]. Nauka i Zdravooh. – Science and Health Care, 3, 5-25 [in Ukrainian].
Zaporozhyan, V.M., & Aryaev, M.L. (2013). Bioetyka ta biobezpeka [Bioethics and biosafety]. Kyiv: Zdorovia [in Ukrainian].
Malyarskay, N.V., & Kalinichenko, M.A. (2017). Endotelialna dysfunktsiya yak universalnyy predyktor rozvytku sertsevo-sudynnoyi patolohiyi ta mozhlyvosti yiyi korektsiyi v praktytsi simeynoho likarya [Endothelial dysfunction as a universal predictor of the development of cardiovascular pathology and the possibility of its correction in the practice of a family doctor]. Liky Ukrainy – Drugs of Ukraine, 1(207), 38-41 [in Ukrainian].
Festing, M., & Nevalainen, T. (2014). The design and statistical analysis of animal experiments introduction to this issue. Ilar J., 55(3) 379-372. DOI: https://doi.org/10.1093/ilar/ilu046
