QUANTITATIVE MORPHOLOGICAL ANALYSIS OF THE FEATURES OF REMODELING OF VENOUS VESSELS OF THE PROSTATE GLAND UNDER CHRONIC ALCOHOL INTOXICATION
DOI:
https://doi.org/10.11603/1811-2471.2022.v.i4.13503Keywords:
prostate gland, venous channel, chronic alcohol intoxication, quantitative morphologyAbstract
SUMMARY. Chronic alcohol intoxication leads to damage to almost all organs and systems. Structural and functional disorders of prostate gland in this pathology have not been sufficiently studied.
The aim – to study the features of remodeling of venous bed of prostate gland in chronic alcohol intoxication using quantitative morphological methods.
Material and Methods. The venous channel of 62 rats, which were divided into 2 groups, was studied. 1-st group – 20 intact, 2-nd group – 42 rats, which, after selection of susceptibility to chronic alcohol intoxication, were injected intragastrically with a 30 % ethanol solution at the rate of 2 ml per 100 g of body weight once a day for 28 days. Animals were euthanized by bloodletting under thiopental anesthesia.
The diameter of postcapillary venules, venules, outer and inner diameters, wall thickness of venous vessels, height of endotheliocytes, diameter of their nuclei, nuclear-cytoplasmic ratio in these cells, relative volumes of damaged endotheliocytes, density of microvessels per 1 mm² of gland tissue were determined on micropreparations of the prostate gland. Quantitative indicators were processed statistically.
Results. The venous vessels of prostate gland were markedly dilated during chronic alcohol intoxication. The thickness of their wall decreased by 26.8 %. The height of vein endotheliocytes decreased by 7.6%, the diameter of nuclei – by 4.6 %, the nuclear-cytoplasmic ratio increased by 6.9 %, the relative volume of damaged endotheliocytes – 9.4 times. The density of microvessels decreased by 7.1 %, indicating impaired hemomicrocirculation. Light-optically revealed pronounced venous congestion, dystrophic necrobiotic changes of endotheliocytes, stromal structures, infiltration and sclerosis.
Conclusions. Chronic alcohol intoxication in laboratory sexually mature white male rats leads to pronounced remodeling of venous bed of prostate gland, which is characterized by expansion of lumen of vessels, thinning of their walls, atrophic, dystrophic and necrobiotic changes in endotheliocytes, disruption of structural cellular homeostasis in them, endothelial dysfunction, hypoxia, dystrophic-necrotic changes in cells, stromal structures, infiltration and sclerosis.
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