MORPHOMETRIC STUDY OF FEATURES OF REMODELING OF COLON STRUCTURES IN THE CONDITIONS OF POSTRESECTION PORTAL HYPERTENSION
DOI:
https://doi.org/10.11603/1811-2471.2022.v.i3.13292Keywords:
postresection portal hypertension, colon, morphometryAbstract
SUMMARY. Removal of large volumes of liver parenchyma often leads to postresection portal hypertension, which is complicated by hypertension, plethora and morphological reorganization of the portal hepatic vein, damage to their structures, changes in the colon in these pathological conditions are insufficiently studied.
The aim – to study the features of remodeling of colon structures in postresection portal hypertension morphometrically.
Material and Methods. The structures of the colon of 67 white male rats, which were divided into 3 groups, were morphometrically studied. The 1-st group consisted of 20 intact animals, the 2-nd – 30 rats with postresection portal hypertension, the 3-rd – 17 animals with postresection portal hypertension and multiorgan failure. Euthanasia of experimental animals was performed by bloodletting under thiopental anesthesia 30 days after the start of the experiment. Histological micropreparations were made from the colon, which were used to determine the thickness of the mucous, muscular, serous membranes of the colon, submucosal layer, submucosal mucosa, musculoskeletal indices, relative volume of epitheliocytes, microvessels, capillary epithelium epitheliocytes, the diameter of their nuclei, nuclear-cytoplasmic relations in these cells, the relative volume of damaged epitheliocytes. Quantitative indicators were processed statistically.
Results. Postresection portal hypertension was complicated by severe remodeling of colon structures, which was characterized by venous plethora, uneven, disproportionate changes in morphometric parameters of the membranes, the relationship between them, decreased capillary-epitheliocyte relations, stromal structures, foci of infiltration, sclerosis with their dominance in the development of multiorgan failure.
Conclusions. Postresection portal hypertension leads to severe remodemation of colon structures, which was characterized by venous plethora, uneven, disproportionate changes in the morphometric parameters of its membranes, the relationship between them, a decrease in capillary-epithelial disorders, disturbance of epithelial cells, miocytes, stromal structures, foci of infiltration, sclerosis. Structural changes in a large intestine at a combination of a postresection portal hypertension with multiorgan insufficiency dominate.
References
Vishnievskiy, V.A., Jefanov, M.H., & Kazakov, J.V. (2012). Siegmientarnye rieziektsyi, otdaliennye rezultaty pri zlokachiestviennykh opukholiakh piechieni [Segmental resections, long-term results in malignant liver tumors]. Ukrainskyi zhurnal khirurhiyi – Ukrainian Journal of Surgery, 1(16), 5-15 [in Ukrainian].
Hnatiuk, M.S., Tatarchuk, L.V., & Yasinovskyy, O.B. (2016). Morfometrychna otsinka osoblyvostey remodeliuvanniia struktur dvanadtsiatypaloi kyshky pry resektsii ruznykh obiemiv pechinky [Morphometric evaluation of the features of remodeling of duodenal structures during resection of different volumes of the liver]. Naukovyi visnyk Uzhhorodskoho universytetu. Seriia “Medytsyna” – Scientific Journal of Uzhhorod University. Series “Medicine”, 1(49), 3-5 [in Ukrainian].
Dzyhal, O.F. (2017). Formuvannya polisyndromnoyi nedostatnosti khvorykh na tsyroz pechinky z portalnoyu hipertenziyeyu [Formation of polysyndromic insufficiency in patients with cirrhosis of the liver with portal hypertension]. Visnyk naukovykh doslidzhen – Bulletin of Scientific Research, 2, 88-92. DOI: 10.11603/2415-8798.2017.2.7844 [in Ukrainian].
Mohylevets, E.V., Horelyk, P.V., & Dubrovschyk, O.Y. (2018). Portalnaya hypertensiya, oslozhnennaya krovotechenijem iz varykozno rasshyrennyh ven pytchevoda i zheludka: sovremennoye sostojanije problemy lechenija j profilaktyky [Portal hypertension complicated by bleeding from varicose veins of the esophagus and the stomach: the current state of the problem of treatment and prevention]. Ukr. zhurnal khirurhiya – Ukr. Journal of Surgery, 1(36), 67-72 [in Ukrainian].
Tatarchuk, L.V, Shulgay, A.G, & Hnatyuk, M.S. (2018). Osoblyvosti energozabezpechennia klubovoi kyshky pry rezekciiach riznych obiemiv pechinky [Features of energy supply of the ileum during resection of different volumes of the liver]. Medychna i klinichna chimija – Medical and Clinical Chemistry, 20 (4), 11-16 [in Ukrainian].
Bosch, J., & Iwakiri, Y. (2017). The portal hypertension syndrome: etiology, classification, relevance, and animal models. Hepatology International, 12(1), 1-10. DOI: 10.1007/s12072-017-9827-9.
Rialon, K.L. Murillo, R., & Fevurtly, R.O. (2015). Impact of screening for hepatic hemangioma in patients with multiple cutaneous infantile hemangiomas. Pediatr. Dermatol., 32 (6), 162-167.
Bahrii, M.M., Dibrova, V.A., Popadynets, O.H., & Hryshchuk, M.I. (2016). Metodyky morfolohichnykh doslidzhen [Methods of morphological research]. Vinnytsia: Nova knyha [in Ukrainian].
Avtandilov, G.G. (2002). Osnovy kolichestviennoy patologicheskoy anatomii. [Fundamentals of Quantitative Pathological Anatomy]. Moskva: Meditsyna [in Russian].
Grzhibovsky, A.I., Ivanov, O.V., & Gorbatova, M.A. (2016). Sravnennia kolichestvennych dannych dvuch par-nych vyborok s ispolzovanijm programnogo obespechenia Statistica i SPSS; parametricheskie and neparametricheski criterii [Comparison of Quantitative Data of two Paired Samples Using Statistica and SPSS Software: Parametric and nonparametric Criteria]. Nauka i zdravokhranenie – Science and Health, 3, 5-25 [in Russian].
Zaporozhyan, V.M., & Aryaev, M.L. (2013). Bioetyka I biobezheka [Bioethics and biosafety]. Kyiv: Zdorovia [in Ukrainian].
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2022 Achievements of Clinical and Experimental Medicine
This work is licensed under a Creative Commons Attribution 4.0 International License.
