THE INFLUENCE OF L-ORNITHINE THE INFLUENCE OF L-ORNITHINE L-ASPARTATE ON THE CONTENT OF TNF-Α AND TGF-ß IN THE LIVER IN EXPERIMENTAL CIRRHOSIS AGAINST THE BLOCKADE OF THE SYNTHESIS OF NITRIC OXIDE AND POLYAMINE

Abstract

SUMMARY. The aim perform an immunohistochemical study of the content of transforming growth factor β (TGF-β) and tumor necrosis factor α (TNF-α) in the liver and to investigate the structure of the organ in experimental CCL4-induced cirrhosis and to investigate the effectiveness of the use of L-ornithine L-aspartate under conditions of blockade of the synthesis of nitric oxide and polyamines.

Material and Methods. Experimental liver cirrhosis was modeled by oral administration of a 50 % oil solution of CCL4 to white Wistar rats for 12 weeks. For the purpose of correction, L-ornithine L-aspartate (LOLA) was used at a dose of 200 mg/kg, as well as LOLA in combination with a non-selective blocker of nitrogen synthesis L-NAME at a dose of 10 mg/kg and a blocker of polyamine synthesis DFMO at a dose of 25 mg/kg. A histological and immunohistochemical study of animal livers was carried out with the determination of growth factor (TGF-β) and tumor necrosis factor (TNF-α).

Results. The formation of CCL4-induced cirrhosis with the formation of pronounced fibrosis along the course of the portal tracts, dystrophic and dystrophic-necrotic changes in hepatocytes, significant lymphohistiocytic infiltration of fibrotic fields, the appearance of TNF-α and TGF-β factors on cell membranes, mainly in the lumen of the sinusoids, was revealed. A significant increase in these indicators was observed under the influence of the non-selective blocker of NO-synthase L-NAME and the inhibitor of polyamine metabolism DFMO.