ACTIVITY OF INFLAMMATORY PROCESSES IN RATS IN EXPERIMENTAL CARCINOGENESIS AND INFLUENCE ON THEM THICK EXTRACT FROM MAYTAKE MUSHROOMS
DOI:
https://doi.org/10.11603/1811-2471.2022.v.i1.12987Keywords:
Maitake mushrooms, experimental chronic endotoxicosis, inflammatory processes, thick extract, oncoprotective actionAbstract
SUMMARY. Today, the problem of finding effective oncoprotectors is particularly acute, as cytostatics act nonspecifically, affecting not only tumor but also healthy cells. This leads to particularly dangerous side effects such as hepatotoxicity, cardiotoxicity, neurotoxicity, nephrotoxicity and suppression of the immune system.
The aim – to study the anti-inflammatory properties of a thick extract of Maitake mushrooms in an experiment on rats with a chemically induced cancer process.
Мaterial and Methods. The study was performed on 120 white rats, which simulated oncopathology by administering 1,2-dimethylhydrazine hydrochloride for 3 weeks (1 time per week). In order to correct the detected violations, a thick extract of Maitake mushrooms was administered intragastrally daily at a dose of 150 mg / kg of animal body weight throughout the experiment. Euthanasia was performed monthly. The serum of rats was taken for research. The level of cytokines, in particular, IL-4, IL-6 and TNF was determined by enzyme-linked immunosorbent assay. The content of C-reactive protein was determined by immunoturbidimetric method.
Results. The content of pro-inflammatory, anti-inflammatory cytokines and C-reactive protein in the serum of rats under the conditions of a simulated cancer process was studied. It was found that a thick extract of Maitake mushrooms significantly reduces the level of pro-inflammatory IL-6, TNF and C-reactive protein, increases the level of anti-inflammatory IL-4 in the serum of rats under conditions of long-term use of carcinogens.
Conclusions. The obtained experimental data show that a thick extract of Maitake mushrooms showed an effective effect on the content of C-reactive protein, pro- and anti-inflammatory cytokines in the serum of animals with prolonged administration of 1,2-dimethylhydrazine hydrochloride. This indicates the anti-inflammatory properties of the studied pharmacological drug in induced carcinogenesis.
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