CHANGES IN ADIPOPYTOKINES AND GRELIN LEVELS IN EXPERIMENTAL ANIMALS WITH METABOLIC SYNDROME
DOI:
https://doi.org/10.11603/1811-2471.2021.v.i4.12803Keywords:
metabolic syndrome, leptin, adiponectin, ghrelinAbstract
SUMMARY. Metabolic syndrome is a complex of pathological conditions, the criteria of which, according to the National Cholesterol Education Program-Adult Treatment Panel III, are three or more of the following disorders: abdominal obesity, fasting hyperglycemia, hypertriglyceridemia, hypertension, low cholesterol. Adipose tissue of the abdominal area plays an important role in the development and progression of insulin resistance and related metabolic disorders.
The aim – to study the level of ghrelin, leptin and adiponectin in the body of experimental animals with a simulated metabolic syndrome.
Material and Methods. The study was performed on 20 white male Wistar rats weighing 220–250 g (age 9–10 weeks). Animals in the control group (n=10) were fed a normal control diet. Male rats of the main group (n=10) were fed a diet high in fat (over 60 % energy from fat) for 16 weeks. All animals in the main group had at least 3 of the 5 MS criteria. The content of leptin and adiponectin was determined by enzyme-linked immunosorbent assay using Bio Vendor kits (Leptin Mouse / Rat Elisa, Adiponectin HMW Mouse / Rat Elisa) (Czech Republic). The level of gerlin was measured by enzyme-linked immunosorbent assay (ELISA) using an enzyme-linked immunosorbent assay from Diagnostic System Laboratories (USA).
Results. When determining the level of adiponectin in our rats, we found a statistically significant decrease in the concentration of adiponectin in rats with MS, compared with the control group – (4.07±0.67) μg/ml vs. (6.15±0.54) μg/ml (p<0.05). Analyzing leptin levels in the compared groups, we found a statistically significant increase in the concentration of leptin in the plasma of rats with MS (35.19 ng/g), compared with the control group (22.58 ng/g) (p<0.05). When determining the level of ghrelin in the studied animals, we found a statistically significant increase in ghrelin levels in rats with MS (3.09 ng/ml) compared with the control (2.20 ng/ml) (p<0.05).
Conclusion. Thus, the simulation of metabolic syndrome in rats leads to a statistically significant increase in ghrelin levels and an imbalance in the production of adipose tissue hormones – leptin and adiponectin (p<0.05).
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