IDENTIFICATION OF SEROLOGICAL MARKERS OF PATHOGENS INFECTIOUS DISEASES IN PATIENTS WITH CARDIOVASCULAR DISEASES

Abstract

SUMMARY. The aim – to clarify the spectrum of serological markers of infectious agents in patients with cardiovascular disease (CVD) using enzyme-linked immunosorbent assay (ELISA) and the multiplex indirect immunofluorescence method in the BIOCIP modification.

Material and Methods. Under supervision there were 69 patients with damage to the cardiovascular system aged 18 to 69 years, who were treated on an outpatient and inpatient basis in the Ternopil University Hospital for CVD pathology. There were 41 (59.4 %) men and 28 (40.6 %) women.

For etiological decoding of LB, the ELISA method was used. Antibodies to the antigens of the B. burgdorferi sensu lato (s. L.) сomplex were determined in blood sera by the ELISA method using test systems from Euroimmun AG (Germany). The results obtained were assessed as positive, intermediate or negative and interpreted according to the manufacturer's recommendations.

In 20 patients out of 69 examined, using the multiplex NIF method, specific antibodies of the IgM and IgG classes were diagnosed to 16 pathogens of infectious diseases that can cause CV diseases. We used test systems "Mosaic: Myocarditis profile 1 (IgM / IgG)", EUROIMMUN, Germany, which contained fluorescein-labeled antigens of pathogens of such infectious diseases: mumps, influenza, parainfluenza, mycoplasma, cytomegalovirus, Coxsackie and enterovirus infections, LB and chlamydia.

This method for the diagnosis of specific antibodies of the IgM and IgG classes to pathogens of infectious diseases in patients with CVS lesions in Ternopil was used for the first time

The results of the determination of antibodies to the causative agents of the above infectious diseases were assessed in the field of view of a fluorescent microscope (Olympus IX70, eyep. × 10, obj. × 20; 40) by the bright green glow of the antigen-antibody immune complex labeled with fluorescein

Results. When analyzing the results of a serological study of blood sera of patients with CVS lesions using ELISA to detect specific antibodies of the IgM and IgG classes to the B. burgdorferi s. l. complex it was found that positive and intermediate results of the presence of antibodies of at least one class were in 34 (49.3 %) of 69 examined patients.

At the same time, specific antibodies of both classes (positive / positive and intermediate / intermediate results) were diagnosed in the sera of 36 (52.2 %) people.

In general, positive results for the presence of IgM antibodies to B. burgdorferi s. l. were obtained in 17 (24.6 %) patients, intermediate – in 6 (8.7 %), negative – in 46 (66.7 %). At the same time, 19 (27.5 %) people had positive results for detecting specific antibodies of the IgG class, intermediate – in 5 (7.2 %), negative – in 45 (65.3 %).

Using the multiplex NIF method, specific IgM antibodies were determined in 10 patients and IgG in 10 patients against 16 infectious agents that can affect CVS. The analysis of the obtained results revealed the following: specific antibodies of the IgM class were found only to 6 of the 16 above-mentioned infectious diseases pathogens.

Conclusions. The method of multiplex indirect immunofluorescence using the BIOCHIP technology is highly informative, since in persons with cardiovascular diseases it has made it possible to simultaneously identify specific IgG to 16 infectious agents, and specific IgM to 6, mainly in various combinations.

In patients with cardiovascular disease, the use of the multiplex NIF reaction supplemented the results of ELISA, a traditional method of serological diagnosis of Lyme borreliosis, allowing the simultaneous determination of antibodies to Borrelia of three genotypes of the B. burgdorferi s. l. complex (B. burgdorferi s. s., B. afzelii, B. garinii).