COMPARATIVE ANALYSIS OF TWO TYPES OF CONTROL IN THE FORMATION OF AN EXPERIMENTAL MODEL OF CHRONIC ULCERATIVE COLITIS
DOI:
https://doi.org/10.11603/1811-2471.2021.v.i4.12799Keywords:
chronic ulcerative colitis, cyclooxygenase, ethanol, dinitrobenzenesulfonic acidAbstract
SUMMARY. The article presents a comparative analysis of two types of control in the remodeling of chronic ulcerative colitis with 2,4-Dinitrobenzenesulfonic acid (DNBS) in 50 % ethanol solution, based on which the authors do not recommend the use of animals with intrarectal ethanol administration as the only control to prevent misinterpretation of the results.
The aim – to сompare two types of control in the reproduction of an experimental model of chronic ulcerative colitis to prevent misinterpretation of the results.
Material and Methods. The analysis of the presence and severity of inflammatory pathological processes in the colon by morphological method and cyclooxygenase-1 and -2 content determination by enzyme immunosorbent assay have been carried out on three groups of sexually mature laboratory rats of both sexes of the WAG population (the first control group – intrarectal administration of 0.9 % saline; the second control group – administration of 50 % ethanol solution; the experimental group – administration of DNBS in 50 % ethanol solution).
Results. Similar morphological patterns and changes in the content of cyclooxygenases in the colon tissue were revealed under the influence of both DNBS dissolved in alcohol and ethanol. There was no difference in these parameters between the experimental and second control groups.
Conclusions. A group of animals with intrarectal administration of ethanol can not be the only control in the model of chronic ulcerative colitis, but can be used to identify effects due to DNBS as hapten and not ethanol as gut barrier “destroyer”.
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