THE ROLE OF INTERLEUKIN-6 AND GAMMA-INTERFERON IN THE REGULATION OF REPARATIVE PROCESSES IN CHRONIC WOUNDS USING PHOTOBIOMODULATION THERAPY
DOI:
https://doi.org/10.11603/1811-2471.2021.v.i3.12528Keywords:
chronic wounds, photobiomodulation therapy, cytokinesAbstract
The search for new methods of treating chronic wounds is an urgent task today. A promising method of influencing the reparation process is the use of photobiomodulation (PBM) therapy.
The aim – to study the role of interleukin-6 (IL-6) and gamma-interferon (IFN-γ) in the regulation of reparative processes in chronic wounds when using PBM therapy in an experiment.
Material and Methods. The studies were carried out on 24 Wistar rats in which chronic wounds were simulated. The animals were divided into two groups – experimental and control. Wound defects of the animals of the experimental group were exposed to PBM therapy once a day for 5 days. We used a laser device Lika-therapist M (Cherkassy, Ukraine) in a continuous mode at a wavelength of 660 nm, an output power of 50 mW, an energy density of 1 J/cm2, an exposure time of 60 s. Blood sampling from the heart was performed on days 3 and 7 after surgery. The study of the levels of IL-6 and IFN-γ in the blood serum of animals was carried out by the method of enzyme-linked immunosorbent assay.
Results. The study showed that 3 days after induction of the wound, the levels of IL-6 in the blood serum of the animals of the experimental group significantly decreased (3.418±0.381 pg/ml, P<0.05) compared with the same indicators in the control group (7.356±0.649 pg/ml). Concentrations of IFN-γ in the serum of animals of both groups at this time of wound healing did not differ.
After 7 days, the levels of IFN-γ in the blood of animals whose wound defects were exposed to the influence of PBM therapy were reduced (103.330±4.493 pg/ml, P<0.05) compared with the concentration of IFN-γ in animals of the control group (155.208±9.574 pg/ml). The levels of IL-6 in the blood serum of the animals of the studied groups did not differ.
Conclusions. The regulation of disturbances in the reparative processes of chronic wounds can be carried out using PBM therapy, although this fact is closely related to the choice of parameters such as dose, exposure time, and wavelength of laser radiation.
A decrease in the production of IL-6 and IFN-γ at the initial stages of injury healing may be one of the mechanisms by which PBM therapy stimulates the processes of chronic wound repair.
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