ACTIVITY OF INFLAMMATORY PROCESSES IN RATS WITH TOXIC PARACETAMOL HEPATITIS AND AFTER APPLICATION OF DRY EXTRACT FROM REISHI MUSHROOMS
DOI:
https://doi.org/10.11603/1811-2471.2021.v.i3.12517Keywords:
Reishi mushrooms, paracetamol, hepatitis, inflammatory processes, dry extract, anti-inflammatory propertiesAbstract
Due to the fact that acute hepatitis can lead to serious consequences, there is a need for early diagnosis and the use of effective hepatoprotectors to treat and prevent complications.
The aim – to investigate the anti-inflammatory properties of dry Reishi mushroom extract in an experiment on rats with toxic hepatitis.
Мaterial and Methods. The experiment was performed on 60 white male rats randomized to 10 groups of 6 animals each. Acute hepatitis was simulated by administration of paracetamol (acetaminophen) intragastrically at a dose of 1250 mg/kg 1 time per day as a suspension in a 2% solution of starch gel for 2 days. Dry Reishi mushroom extract was administered intragastrally 2 hours before acetaminophen administration and daily after the lesion at a dose of 100 mg/kg of body weight to correct the toxic lesion. “Silibor” (manufacturer – LLC "Pharmaceutical Company" Health ") was chosen as a comparison drug and administered according to the same regimen as reishi extract at a dose of 20 mg/kg of rat body weight. Euthanasia was performed on the 3rd, 7th and 10th day from the onset of the lesion. Liver homogenate and blood serum were used for the studies. The dynamics of inflammatory processes was studied by the content of pro-, anti-inflammatory cytokines, C-reactive protein in the serum of animals with paracetamol hepatitis, as well as after the corrective effect of Reishi mushroom extract and the comparison drug.
Results. The content of pro-inflammatory, anti-inflammatory cytokines and C-reactive protein in the serum of rats under conditions of paracetamol hepatitis was studied. It was found that the dry extract of Reishi mushrooms significantly reduces the level of pro-inflammatory IL-6 and C-reactive protein, increases the level of anti-inflammatory IL-4 in the serum of rats under conditions of toxic lesions.
Conclusions. The obtained results indicate the suppression of inflammatory processes under the influence of dry extract of Reishi mushrooms under conditions of paracetamol hepatitis in rats. The anti-inflammatory properties of the studied extract are one of the mechanisms of its stress-adaptogenic and hepatoprotective action.
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