MORPHOLOGICAL CHARACTERISTICS OF GUINEA PIGS BRONCHUS ASSOCIATED LYMPHOID TISSUE IN THE DYNAMICS OF EXPERIMENTAL OVALBUMIN-INDUCED ALLERGIC INFLAMMATION
DOI:
https://doi.org/10.11603/1811-2471.2021.v.i3.12507Keywords:
Bronchus associated lymphoid tissue, experimental allergic inflammation, ovalbumin, guinea pigAbstract
The actual problem of modern medicine is the reaction of Bronchus associated lymphoid tissue in airways chronic allergic diseases, because the full functioning of the respiratory system depends on the state of its local immune system.
The aim – to study morphological changes in Bronchus associated lymphoid tissue of guinea pigs in the dynamics of experimental ovalbumin-induced allergic inflammation.
Material and Methods. We have studied the lung of 48 guinea pigs, using histological and morphometric methods, under conditions of experimental ovalbumin-induced allergic inflammation. To assess the structural and functional rearrangement of pulmonary lymphoid nodules, their diameter and number were determined.
Results. We have shown that sensitization and allergization with ovalbumin leads to a significant structural and functional rearrangement of Bronchus associated lymphoid tissue of guinea pigs in the form of the elevation in their number and diameter. We found more pronounced morphological changes in the late period of development of allergic inflammation in peribronchial lymphoid nodules, confirmed by the maximum magnification factor – 2.6 (p*/**<0.001) of the diameter of peribronchial lymphoid nodules in the 4th experimental group compared to the control.
Conclusions. Sensitization and inhalation with ovalbumin leads to structural reorganization of Bronchus associated lymphoid tissue of guinea pigs in the form of the elevation in their number and diameter, had the unidirectional staged character depending on localization of lymphoid nodules and the duration of the experiment and claimed proliferative changes in the cellular adaptive link of local lung immunity of guinea pigs in late period of allergic inflammatory process.
References
Hrebniak, M.P., & Fedorchenko, R.A. (2019). Influence of industrial atmospheric pollution on the development of pathology of respiratory organs. Pathologia, 16 (1), 81-86. DOI: https://doi.org/10.14739/2310-1237. 2019.1.166314.
Heier, I., Malmstrom, K., Pelkonen, A.S., Malmberg, L.P., Kajosaari, M., Turpeinen, M., ..., & Makela, M.J. (2008). Bronchial response pattern of antigen presenting cells and regulatory T cells in children less than 2 years of age. Thorax, 63 (8), 703-709. DOI: https://doi.org/10.1136/thx.2007.082974.
Lambrecht, B.N., & Hammad, H. (2014). The immunology of asthma. Nat. Immunol., 16 (1), 45-56. DOI: https://doi.org/10.1038/ni.3049.
Hwang, J.Y., Randall, T.D., & Silva-Sanchez, A. (2016). Inducible bronchus-associated lymphoid tissue: taming inflammation in the lung. Front. Immunol., 7 (258). DOI: https://doi.org/10.3389/fimmu.2016.00258.
Shilling, R.A., Williams, J.W., Perera, J., Berry, E., Wu, Q., Cummings, O.W., ..., & Huang, H. (2013). Autoreactive T and B cells induce the development of bronchus-associated lymphoid tissue in the lung. Am. J. Respir. Cell Mol. Biol., 48 (4), 406-414. DOI: https://doi.org/10.1165/rcmb. 2012-0065oc.
Popko, S.S. (2021). Morphological rearrangement of the metabolic link of the microcirculatory bed of guinea pigs lungs after sensitization with ovalbumin. Current Issues in Pharmacy and Medicine: Science and Practice, 14 (1), 79-83. DOI: https://doi.org/10.14739/2409-2932.2021.1.226851.
Cai, Z., Liu, J., Bian, H., & Cai, J. (2019). Albiflorin alleviates ovalbumin (OVA)-induced pulmonary inflammation in asthmatic mice. Am. J. Transl. Res., 11 (12), 7300-7309. DOI: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6943473/.
Zemmouri, H., Sekiou, O., Ammar, S., El Feki, A., Bouaziz, M., Messarah, M., & Boumendjel, A. (2017). Urtica dioica attenuates ovalbumin-induced inflammation and lipid peroxidation of lung tissues in rat asthma model. Pharm. Biol., 55 (1), 1561-1568. DOI: https://doi.org/10.1080/13880209.2017.1310905.
Antwi, A.O., Obiri, D.D., & Osafo, N. (2017). Stigmasterol modulates allergic airway inflammation in guinea pig model of ovalbumin-induced asthma. Mediators Inflamm., 2017, 2953930. DOI: https://doi.org/10.1155/2017/2953930.
Sui, P., Wiesner, D.L., Xu, J., Zhang, Y., Lee, J., Van Dyken, S., ..., & Sun, X. (2018). Pulmonary neuroendocrine cells amplify allergic asthma responses. Science, 360(6393), eaan8546. DOI: https://doi.org/10.1126/science.aan8546.
Popko, S.S., Evtushenko, V.M., & Syrtsov, V.K. (2020). Influence of pulmonary neuroendocrine cells on lung homeostasis. Zaporozhye Medical Journal, 22 (4), 568-575. DOI: https://doi.org//10.14739/2310-1210.4.208411.
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