INFLUENCE OF ACUTE BLOOD LOSS COMPLICATED BY LIMB ISCHEMIA-REPERFUSION ON THE DYNAMICS OF ENZYME LINKS OF ANTIOXIDANCE ZADACANT
DOI:
https://doi.org/10.11603/1811-2471.2021.v.i2.12225Keywords:
острая кровопотеря, ишемия-реперфузия конечности, селезенка, супероксиддисмутаза, каталаза, карбацетамAbstract
Violation of the enzymatic link of antioxidant protection belongs to one of the key pathogenic mechanisms of damage to internal organs in conditions of acute blood loss and ischemic-repefusion syndrome. However, disorders of superoxide dismutase [SOD) and catalase in acute blood loss and its complications by ischemia-reperfusion of the limb in the spleen have not been studied. There are no data on the effectiveness under these conditions of carbacetam, which has antioxidant and tissue-protective activity.
The aim – to find out the features of disorders of the enzyme link of the antioxidant system in the spleen of rats after acute blood loss complicated by ischemia-reperfusion of the limb, and the effectiveness of its correction by carbacetam.
Material and Methods. 108 nonlinear male rats weighing 200–220 g were used in the experiments. All experiments were performed under thiopental-sodium anesthesia. In animals, limb ischemia-reperfusion and acute blood loss were simulated and these lesions were combined. In a separate group, the detected disorders were corrected with carbacetam. After 1 and 2 hours, as well as after 1, 7 and 14 days in the spleen of experimental animals, the activity of SOD and catalase was determined.
Results. Simulation of acute blood loss complicated by ischemia-reperfusion of the limb in the spleen contributes to the greatest depletion of SOD and catalase. Starting from 1 h of the experiment, the activity of these enzymes becomes significantly lower compared to animals in the experimental group, which simulated only acute blood loss. The use of carbacetam significantly reduced the activity of SOD and catalase in the spleen after 7 days of use. The rates were even higher after the introduction of carbacetam for 14 days, which indicates a pronounced protective effect of the drug on the studied factors of antioxidant protection of the spleen and is a promising means of systemic correction.
Conclusions. 1. Two-hour ischemia of the limb due to the use of a tourniquet in acute blood loss contributes to a significant decrease in the activity of SOD and catalase in the spleen with a maximum after 1 day, the value of which does not return to the control level after 14 days. Administration of carbacetam for 7–14 days is accompanied by a pronounced protective effect on the enzyme level of antioxidant protection of the spleen.
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