MODEL OF EXTREMITY MICROANGIOPATHY IN STREPTOZOCIN DIABETES
DOI:
https://doi.org/10.11603/1811-2471.2020.v.i4.11769Keywords:
diabetic microangiopathyAbstract
SUMMARY. The development of models of diabetic microangiopathy of the extremities remains relevant. This complication is observed in all patients with severe diabetes, and according to some researchers occurs even in subcompensation in all patients with type 2 diabetes, and in 30 % – with type 1 diabetes.
The aim – to assess the adequacy to the needs of clinical and physiological studies of the experimental model of limb microangiopathy in streptozocin diabetes.
Material and Methods. Experimental studies were performed in a chronic experiment on 50 mature Wistar rats weighing 180–250 g. After administration of streptozocin from the experiment excluded animals with increased resistance to pancreatotropic toxicity in the absence of hyperglycemia, and then for 6 weeks for 2 weeks of withdrawal morphological studies. At 7th week, ten other animals were removed from the experiment to evaluate the effectiveness of the experimental model. The condition of the remaining 10 animals was observed for another three weeks.
During the experiment, laboratory monitoring of glucosuria, acetonuria, proteinuria, glycemia was performed. The formation of DMA was confirmed by morphological examination of the soft tissues of the hind legs of rats.
Statistical processing was performed by nonparametric methods using Statistica 10.0 software (Dell StatSoft Inc., USA).
Results. The average weight of animals involved in the study was (190.0±0.6) g at cv=2.9 %. When analyzing the distribution of blood glucose values in animals after the introduction of streptozocin, it was found that the amplitude of the values of 9.9–17.0 mmol/l on average, this figure was (14.2±0.2) mmol/l. Further analysis showed that the efficiency of ECD modeling was 92 %, which is acceptable for subacute experiment.
Conclusions. It is shown that the developed pathophysiological model of diabetic microangiopathy is adequate to the needs of clinical physiology. In diabetic microangiopathy, remodeling of the capillary bed is observed, which is accompanied by a decrease in vascular diameter to (7.7±0.7) μm, as well as the phenomena of perivascular infiltration and lymphatic drainage dysfunction. The expediency of developing effective methods of indirect revascularization aimed at restoring perfusion and reducing the manifestations of diabetic microangiopathy is discussed.
References
Mazo, V.K., Sidorova, Yu.S., Zorin, S.N., & Kochetkova, A.A. (2016). Streptozototsinovyye modeli sakharnogo diabeta [Streptozotocin models of diabetes mellitus]. Vopr. Pitan. – Nutrition Issues, 85 (4), 14-21 [in Russian].
Furman, B.L. (2015). Streptozotocin-induced diabetic models in mice and rats. Curr. Protoc. Pharmacol., 70 (5), 47.
Al-Awar, A., Kupai, K., Veszelka, M., Szűcs, G., Attieh, Z., Murlasits, Z., …, & Varga, C. (2016). Experimental diabetes mellitus in different animal models. J. Diabetes Res., 2016, 9051426.
King, A., & Bowe, J. (2016). Animal models for diabetes: Understanding the pathogenesis and finding new treatments. Biochem. Pharmacol., 99, 1-10.
King, A.J. (2012). The use of animal models in diabetes research. Br. J. Pharmacol., 166 (3), 877-894.
Barrett, E.J., Liu, Z., Khamaisi, M., King, G.L., Klein, R., Klein, B.E.K., …, & Casellini, C.M. (2017). Diabetic microvascular disease: An Endocrine Society Scientific Statement. J. Clin. Endocrinol. Metab., 102 (12), 4343-4410.
Dunford, E.C., Leclair, E., Aiken, J., Mandel, E.R., Haas, T.L., Birot, O., & Riddell, M.C. (2017). The effects of voluntary exercise and prazosin on capillary rarefaction and metabolism in streptozotocin-induced diabetic male rats. J. Appl. Physiol. (1985), 122 (3), 492-502.
Heinonen, S.E., Genové, G., Bengtsson, E., Hübschle, T., Åkesson, L., Hiss, K., …, & Gomez, M.F. (2015). Animal models of diabetic macrovascular complications: key players in the development of new therapeutic approaches. J. Diabetes Res., 2015, 404085.
Zvershanovskiĭ, F.A., Zhulkevich, I.V., Danilishina, V.S., & Zhulkevich, G.D. (1987). Indicators of lipid metabolism and their relation to disorders of microcirculation in diabetes mellitus. Probl. Endokrinol., 33 (4), 15-18.
Pleshanov, Ye.V. (1990). Patomorfogenez diabeticheskikh mikroangiopatiy, ikh patogeneticheskaya profilaktika i terapiya [Pathomorphogenesis of diabetic microangiopathies, their pathogenetic prevention and therapy]. Doctor᾽s thesis. Kyiv [in Russian].
Nerlich, A. (1995). Morphologie von Basalmembran- und assoziierten Matrixproteinen in normalem und pathologischem Gewebe. Veroff Pathol., 145, 1-139.
Sun, J., Xie, J., Kang, L., Ferro, A., Dong, L., & Xu, B. (2016). Amlodipine ameliorates ischemia-induced neovascularization in diabetic rats through endothelial progenitor cell mobilization. Biomed. Res. Int., 2016, 3182764.
Vaĭnsteĭn, S.G., Zhulkevich, I.V., Dubkin, M.S., & Cherno, N.K. (1987). Food fibers as modifiers of homeostasis in patients with diabetes mellitus. Ter. Arkh., 59 (11), 29-31.
Shved, M.I., Dudnik, A.P., & Zhulkevych, I.V. (1998). Patohenetychni aspekty formuvannia diabetychnykh anhiopatii [Pathogenetic aspects of the formation of diabetic angiopathies]. Visnyk nauk. doslidzhen – Bull. Sci. Res., 1-2, 56-58 [in Ukrainian].
Kundiiev, Yu.I. (Ed.). (2003). Antolohiia bioetyky [Anthology of Bioetics]. Lviv: Bak [in Ukrainian].
Kishkun, A.A. (2008). Klinicheskaya laboratornaya diagnostika [Clinical laboratory diagnostics]. Moscow: GEOTAR-Media [in Russian].
Borovykov, V.P., & Borovykov, Y.P. (1998). Statistica – Statystycheskyy analiz i obrabotka dannykh v srede Windows [Statistical analysis and data processing in the Windows environment]. Moscow: Informats.-izdat. dom «Fylyn» [in Russian].
Zhulkevich, I.V., & Vaĭnsteĭn, S.G. (1989). Eksperimentalnoye i klinicheskoye obosnovaniye primeneniya pishchevykh volokon v lechenii sakharnogo diabeta [Experimental and clinical substantiation of the use of dietary fiber in the treatment of diabetes mellitus]. Proceedings from Vsesoyuzna konf. «Khimiya pishchevykh dobavok» – All-Union Conf. ‘‘Chemistry of food additives’’. Chernivtsi [in Russian].
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