MODEL OF EXTREMITY MICROANGIOPATHY IN STREPTOZOCIN DIABETES
DOI:
https://doi.org/10.11603/1811-2471.2020.v.i4.11769Keywords:
diabetic microangiopathyAbstract
SUMMARY. The development of models of diabetic microangiopathy of the extremities remains relevant. This complication is observed in all patients with severe diabetes, and according to some researchers occurs even in subcompensation in all patients with type 2 diabetes, and in 30 % – with type 1 diabetes.
The aim – to assess the adequacy to the needs of clinical and physiological studies of the experimental model of limb microangiopathy in streptozocin diabetes.
Material and Methods. Experimental studies were performed in a chronic experiment on 50 mature Wistar rats weighing 180–250 g. After administration of streptozocin from the experiment excluded animals with increased resistance to pancreatotropic toxicity in the absence of hyperglycemia, and then for 6 weeks for 2 weeks of withdrawal morphological studies. At 7th week, ten other animals were removed from the experiment to evaluate the effectiveness of the experimental model. The condition of the remaining 10 animals was observed for another three weeks.
During the experiment, laboratory monitoring of glucosuria, acetonuria, proteinuria, glycemia was performed. The formation of DMA was confirmed by morphological examination of the soft tissues of the hind legs of rats.
Statistical processing was performed by nonparametric methods using Statistica 10.0 software (Dell StatSoft Inc., USA).
Results. The average weight of animals involved in the study was (190.0±0.6) g at cv=2.9 %. When analyzing the distribution of blood glucose values in animals after the introduction of streptozocin, it was found that the amplitude of the values of 9.9–17.0 mmol/l on average, this figure was (14.2±0.2) mmol/l. Further analysis showed that the efficiency of ECD modeling was 92 %, which is acceptable for subacute experiment.
Conclusions. It is shown that the developed pathophysiological model of diabetic microangiopathy is adequate to the needs of clinical physiology. In diabetic microangiopathy, remodeling of the capillary bed is observed, which is accompanied by a decrease in vascular diameter to (7.7±0.7) μm, as well as the phenomena of perivascular infiltration and lymphatic drainage dysfunction. The expediency of developing effective methods of indirect revascularization aimed at restoring perfusion and reducing the manifestations of diabetic microangiopathy is discussed.
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