PECULIARITIES OF DISORDERS OF ENZYME LINKS OF ANTIOXIDANT SYSTEM IN LUNGS OF RATS AFTER ACUTE BLOOD LOSS, COMPLICATED BY ISCHEMIA-REPERFUSION OF THE LIMB
DOI:
https://doi.org/10.11603/1811-2471.2020.v.i3.11596Keywords:
acute blood loss, ischemia-reperfusion of the limb, superoxide dismutase, catalase, lungsAbstract
One of the key pathogenic mechanisms of massive external blood loss from the extremities is the activation of lipid peroxidation, due on the one hand to the development of hypoxia, on the other – the depletion of antioxidant protection. The use of a tourniquet under these conditions is accompanied by ischemic-reperfusion injury of the limb, which complicates the course of acute blood loss. To date, there is insufficient data on the features of antioxidant defense disorders in the lungs, which belong to the key target organs of acute blood loss and ischemic-reperfusion syndrome.
The aim – to determine the features of disorders of the enzyme link of the antioxidant system in the lungs of rats after acute blood loss complicated by ischemia-reperfusion of the limb.
Material and Methods. The study was performed on 156 white nonlinear male rats weighing 200–220 g. All experiments were performed under thiopental-sodium anesthesia. In animals, limb ischemia-reperfusion and acute blood loss were simulated and these lesions were combined. After 1 and 2 hours, as well as after 1, 7 and 14 days in the lung homogenates of the experimental animals was determined by the activity of superoxide dismutase and catalase.
Results and Discussion. Under the conditions of modeling only ischemia-reperfusion of the limb, the activity of SOD and catalase in the lung homogenate increased with a maximum after 2 h of the experiment and normalization of values up to 14 days. Under conditions of acute blood loss, the indicators increased in all terms of observation with a maximum after 1 day of the experiment. Additional ischemia-reperfusion of the limb on the background of acute blood loss in the lungs contributes to the depletion of SOD and catalase, the activity of which after 1, 7 and 14 days becomes significantly less than in the animal experimental group, which simulated only blood loss.
Conclusions. Complications of acute blood loss by ischemia-reperfusion of the limb contribute to greater depletion in the lungs of the enzymatic link of antioxidant protection after 1, 7 and 14 days of the experiment, showing a significant decrease in SOD and catalase activity in the lungs compared to animals simulated only acute blood loss.
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