ANALYSIS OF MELATONIN RECEPTORS TYPE 1A IN NEURONS OF THE ANTERIOR-LATERAL PREOPTIC NUCLEUS OF HYPOTHALAMUS OF RATS UNDER DIFFERENT LIGHT MODE
DOI:
https://doi.org/10.11603/1811-2471.2020.v.i3.11581Keywords:
sleep-wake cycle, anterior-lateral preoptic nucleus of the hypothalamus, chronorhythmsAbstract
The most significant and noticeable biological rhythm observed in the human body is the sleep-wake rhythm and associated changes in physical and mental processes. A convincing criterion for the participation of the anterior-lateral preoptic nucleus of hypothalamus in the temporal organization is the presence of melatonin receptors in them.
The aim – to investigate the effect of a different light mode on the quantitative characterization of the density of specific staining of type 1A melatonin receptors in neurons of the anterior-lateral preoptic nucleus of the rat hypothalamus.
Material and Methods. The experiments were performed on mature white nonlinear male rats. The experimental animals were divided into three groups, each of which, in turn, consisted of two subgroups (six animals). The animals of the first group were kept for seven days in standard light conditions. The animals of the second group, during the same period, were in constant darkness. The third group consisted of rats, which were in the conditions of round-the-clock lighting for seven days. Quantitative differences were found according to the intensity of the specific (immunohistochemical) staining, which was determined by computer microdensitometry in relative units of optical density in the range from "0" to "1".
Results. Specific immunohistochemical staining for type 1A melatonin receptors could be observed in all neurocytes of the anterior-lateral preoptic nucleus of the hypothalamus, regardless of their location on the nucleus. The staining was mainly fine-grained or almost diffuse in nature and covered the membrane of neurons and their cytoplasm. Observing the intensity of immunohistochemical staining from one neurocyte to another, it can be stated that it had different strength both in the membrane and in the cytoplasm.
Conclusions. The optical density of staining for melatonin receptors 1A in the neurons of the anterior-lateral preoptic nucleus of the hypothalamus increases during the night and reaches its maximum at 2 AM. Under conditions of light stimulation, the intensity of the optical density decreases sharply.
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