MORPHOMETRIC, INSTRUMENTAL AND LABORATORY PARAMETERS OF FEMALE RATS WITH EXPERIMENTAL METABOLIC SYNDROME
DOI:
https://doi.org/10.11603/1811-2471.2020.v.i3.11578Keywords:
rat, obesity, metabolic syndrome, arterial blood pressure, dyslipidemiaAbstract
The aim of the study – to learn the dynamics of changes of morphometric, instrumental and laboratory parameters in mature females rats with experimental metabolic syndrome.
Material and Methods. 20 females of white, mature laboratory rats, aged 18–20 months were divided into 2 groups. The first one is an experimental group: 13 female rats with experimental metabolic syndrome; the second one – control group: 7 intact rats, with standard food and water regime. When working with animals, the standards of the Council of Europe Bioethics Convention 1997, the European Convention for the Protection of Vertebrate Animals were observed. Instruments used during scientific research were subject to metrological control.
The simulation of the metabolic syndrome occurred during 60 days. The females supported a special high-calorie diet (grain with margarine 82 % milk fat, corn and sunflower seeds). The water regime included a 20 % solution of fructose and regular water ad libitum, with change every other day. Also, during the first and the fourth weeks of the experiment, the female daily subcutaneously administered Dexamethasone solution at a dosage of 0.1 mg/kg.
Results. Since the beginning of the experiment, female rats who received a special high-calorie diet showed a statistically significant increase in all morphometric and instrumental indexes compared to similar rats in the control group. An increase in body weight in the experimental group was found to be 28.93 % higher than the original weight, was observed arterial hypertension (141/85±5) mmHg, dyslipidemia: elevated total cholesterol (5.37±0.33) mmol/L and TG (2.55±0.24) mmol/L; elevated level glucose (8.52±0.17) mmol/L. The above indicators are criteria indicating the presence of metabolic syndrome in animals under study.
Conclusions. The proposed model of experimental metabolic syndrome, which includes subcutaneous administration of Dexamethasone solution at a dosage of 0.1 mg/kg in the first and the fourth weeks of experiment, with a special high calorie diet and a 20 % solution of fructose, is an effective way to reproduce the metabolic syndrome in small rodents.
References
Shalitin, S., Battelino, T., & Moreno, L.A. (2019). Obesity, metabolic syndrome and nutrition. Nutrition and Growth. B. Koletzko, R. Shamir, D. Turck, M. Phillip (Eds.). World. Rev. Nutr. Diet. Basel, 119, 13-42. Karger: DOI: https://doi.org/10.1159/000494306. DOI: https://doi.org/10.1159/000494306
Neri, C., Di Cesare, C., Labianca, A., Viggiano, M., Caruso, A., & Paradisi, G. (2018). Obesity in pregnancy as a model to identify women at risk for later metabolic syndrome. Gynecol. Endocrinol., 34 (1), 28-31. DOI: https://doi.org/10.1080/09513590.2017.1342792
Michou, M., Panagiotakos, D.B., & Costarelli, V. (2018). Low health literacy and excess body weight: A systematic review. Cent. Eur. J. Public Health, 26 (3), 234-241. DOI: https://doi.org/10.21101/cejph.a5172
Grieger, J.A., Bianco-Miotto, T., Grzeskowiak, L.E., Leemaqz, S.Y., Poston, L., McCowan, L.M., & Roberts, C.T. (2018). Metabolic syndrome in pregnancy and risk for adverse pregnancy outcomes: A prospective cohort of nulliparous women. PLoS Med., 15 (12), e1002710. DOI: https://doi.org/10.1371/journal.pmed.1002710
Hull, H.R., Thornton, J.C., Ji Y., Paley, C., Rosenn, B., Mathews, P., & Gallagher, D. (2011). Higher infant body fat with excessive gestational weight gain in overweight women. Am. J. Obstet. Gynecol., 205 (3), 211-e1. DOI: https://doi.org/10.1016/j.ajog.2011.04.004
Korotchuk, Y.V. (2019). Dynamics of the mass-growth indexes of rats, got from mothers with experimental metabolic syndrome. German Sci. Herald, 2, 35-38.
Thunga, S., & Bampoe, S. (2019). Obesity in pregnancy. Obstetric Anesthesiology: A Case-Based Approach. Husain T., Fernando R., Segal S. (Eds.). Cambridge: Cambridge University Press. DOI: https://doi.org/10.1017/9781316155479. DOI: https://doi.org/10.1017/9781316155479
Siega-Riz, A.M., & Laraia, B. (2006). The implications of maternal overweight and obesity on the course of pregnancy and birth outcomes. Matern. Child Health J., 10 (1), 153-156. DOI: https://doi.org/10.1007/s10995-006-0115-x
Knight-Agarwal, C.R., Williams, L.T., & Davis, D. (2016). Association of BMI and interpregnancy BMI change with birth outcomes in an Australian obstetric population: a retrospective cohort study. BMJ Open, 6, e010667. DOI: https://doi.org/10.1136/bmjopen-2015-010667
Kolesnyk, Y.M., Hancheva, O.V., Abramov, A.V., Kolesnyk, M.Y., Ivanenko, T.V., Tishсhenko, S.V., & Fedotova, M.I. (2017). Suchasni pidkhody i novi metodychni mozhlivosti v otsiniuvanni funktsionalnoho stanu dribnykh laboratornykh tvaryn [Current approaches and new methodological possibilities in the assessment of the functional state of small laboratory animals]. Patolohiia – Pathology, 14 (3), 364-370 [in Ukrainian].
Glantz, S.A. (2012). Primer of biostatistics. 7th ed. New York, NY: McGraw-Hill.
Novelli, E.L., Diniz, Y.S., Galhardi, C.M., Ebaid, G.M., Rodrigues, H.G., Mani, F., ..., & Novelli Filho, J.L. (2007). Anthropometrical parameters and markers of obesity in rats. Lab. Anim., 41 (1), 111-119. DOI: https://doi.org/10.1258/002367707779399518
Campos, KE.D., Volpato, G.T., Calderon, ID.MP., Rudge, MV.C., & Damasceno, D.C. (2008). Effect of obesity on rat reproduction and on the development of their adult offspring. Braz. J. Med. Biol. Res., 41 (2), 122-125. DOI: https://doi.org/10.1590/S0100-879X2008005000001
Shen, Y., Li, W., Leng, J., Zhang, S., Liu, H., Li, W., & Yang, X. (2019). High risk of metabolic syndrome after delivery in pregnancies complicated by gestational diabetes. Diabetes Res. Clin. Pract., 150, 219-226. DOI: https://doi.org/10.1016/j.diabres.2019.03.030
Tangren, J.S., Powe, C.E., Ecker, J., Bramham, K., Ankers, E., Karumanchi, S.A., & Thadhani, R. (2018). Metabolic and hypertensive complications of pregnancy in women with nephrolithiasis. Clin. J. Am. Soc. Nephrol., 13 (4), 612-619. DOI: https://doi.org/10.2215/CJN.12171017
