PECULIARITIES OF QUANTITATIVE MORPHOLOGICAL CHANGES OF ARTERIES OF THE COLON AT RESECTIONS OF DIFFERENT VOLUMES OF LIVER PARENCHYMA
DOI:
https://doi.org/10.11603/1811-2471.2020.v.i3.11391Keywords:
liver resection, large intestine, arteries, morphometryAbstract
Removal of large volumes of liver leads to portal hypertension, bleeding from varicose veins of the esophagus, stomach, rectum, ascites, splenomegaly and structural rearrangement of the organs of the portal hepatic vein. The large intestine is an organ from which venous drainage is performed through the portal hepatic vein and hemodynamic disorders in it are complicated by various structural and functional changes, and the morphology of the arterial bed of the large intestine at resection of various volumes of the liver is insufficiently studied.
The aim of the study – quantitative morphological methods to study the features of the structural rearrangement of the arteries of the large intestine at resection of different volumes of the liver parenchyma.
Material and Methods. Morphologically examined arteries of the colon of 60 rats, which were divided into 3 groups. Group 1 – 20 intact animals, 2 – 20 rats after removal of 31.5 % of the liver parenchyma, 3 – 20 animals after resection of 58.1 % of the liver parenchyma. Euthanasia of animals was performed by bloodletting under thiopental anesthesia 1 month after the start of the experiment. Micronutrents were made from the large intestine, which morphometrically determined the outer and inner diameters of arteries of medium and small calibers, media thickness, adventitia, height of endothelial cells, diameter of their nuclei, nuclear-cytoplasmic ratios in these cells, relative volume of damaged cells. Quantitative indicators were processed statistically.
Results. It was found that the removal of 58.1% of the liver parenchyma led to the development of postresection portal hypertension and severe structural rearrangement of mainly small arteries of the large intestine, which was characterized by a significant increase in wall thickness, media and adventitia, a decrease in lumens by 26.6 %, Kernogan index – in 52.3 % (p <0.001). Nuclear-cytoplasmic ratios in endotheliocytes increased by 7.5 % (p <0.01), the relative volume of damaged endotheliocytes – 14.5 times (p <0.001).
Conclusions. Removal of the left and right lateral lobes of the liver in white rats leads to postresection portal hypertension and severe remodeling of mainly small arteries of the large intestine, which is characterized by thickening of their wall, narrowing of the lumen, decreased Kernogan index, blood disturbances, endothelial cells, endothelial cell lesions dystrophy, necrosis of tissues and cells, focal cellular infiltrates, sclerosis.
References
Avtadnilov, G.G. (2002). Osnovy kolichestvennoy patologicheskoy anatomii [Basis of Quantitative Pathological Anatomy]. Moscow: Meditsyna [in Russian].
Volchenko, I.V., Lykhman, V.M., Skoryy, D.I., & Shevchenko, A.M. (2016). Osoblyvosti vykonannya obshchyrnykh rezektsiy pechinky z urakhuvanniam profilaktyky pisliaoperatsiinykh uskladnen [Peculiarities of performing extensive liver resections taking into account the prevention of postoperative complications]. Kharkivska khirurhichna shkola – Kharkiv Surgical School, 3 (78), 35-39 [in Ukrainian].
Garbuzenko, D.V. (2014). Morfofunktsionalnaya perestroyka pechenochnogo sosudistogo rusla v patogeneze portalnoy gipertenzii pri tsirroze pecheni [Morphofunctional rearrangement of the hepatic vasculature in the pathogenesis of portal hypertension in liver cirrhosis]. Ter. Arkh. – Ter. Archive, 86 (2), 90-95 [in Russian].
Hnatiuk, M.S., Tatarchuk, L.V. Yasinovskyy, O.B. (2016). Morfometrychna otsinka osoblyvostei remodeliuvannia struktur dvanadtsiatypaloi kyshky pry resektsii riznykh obiemiv pechinky [Morphometric evaluation of the features of remodeling of duodenal structures during resection of different volumes of the liver]. Naukovyi visnyk Uzhhorodskoho universytetu. Seriia “Medytsyna” – Scientific Herald of Uzhhorod University. Series "Medicine", 1 (49), 3-5 [in Ukrainian].
Goralsky, L.P., Khomich, V.T., & Kononsky, O.I. (2011). Osnovy histolohichnoi tekhniky i morfofunktsionalni metody doslidzhen u normi i pry patolohii [Fundamentals of histological technique and morphofunctional research methods in normal and in pathology]. Zhytomyr: Polissia [in Ukrainian].
Grzhibovskiy, A.I., Ivanov, O.V., & Gorbatova, M.A. (2016). Sravneniye kolichestvennykh dannykh dvukh parnykh vyborok s vnedreniyem programmnogo obespecheniya Statistica i SPSS: parametricheskiye i neparametricheskiye kriterii [Comparison of quantitative data of two paired samples using Statistica and SPSS software: parametric and nonparametric criteria]. Nauka i zdravookhraneniye – Science and Healthcare, 3, 5-25 [in Russian].
Dzyhal, O.F. (2017). Formuvannia polisyndromnoi nedostatnosti khvorykh na tsyroz pechinky z portalnoiu hipertenziieiu [Formation of polysyndromic insufficiency of patients with liver cirrhosis with portal hypertension]. Visnyk naukovykh doslidzhen – Bulletin of Scientific Research, 2, 88-92 [in Ukrainian].
Dronov, O.I., Zemskov, S.V., Bakunets, Y.P., & Bakunets, P.P. (2016). Khirurgicheskoye lecheniye ochagovogo porazheniya pecheni analiz rezultatov i perspektivy [Surgical treatment of focal liver disease: analysis of results and prospects]. Klinicheskaya khirurgiya – Clinical Surgery, 1, 28-31 [in Ukrainian].
Kolesnik, O.O., Burlaka, A.A., Lukashenko, A.V., & Pryymak, V.V. (2015). Dosvid vykonannia rezektsii pechinky na metastatychnyi kolorektalnyi rak [Experience of liver resection for metastatic colorectal cancer]. Klinichna onkolohiia – Clinical Oncology, 2 (8), 8-13 [in Ukrainian].
Okhotnikova, O.M., Ponochevna, O.V., & Mellina, K.V. (2017). Endotelialna dysfuntsiia yak faktor rozvytku tiazhkoho perebihu prohnozu systemnykh vaskulitiv u ditei [Endothelial dysfunction as a factor in the development of severe prognosis of systemic vasculitis in children]. Klinichna immunolohiia. Alerholohiia. Infektolohiiа – Clinical Immunology. Allergology. Infectology, 2 (99), 46-52 [in Ukrainian].
Feng, R.T., & Weng, K.I. (2015). Molecular mechanism of low doze ionizing radiation in order to control bionegative effects to the organism and related human diseases International Journal of Radiation Biology, 91, 13-27. DOI: https://doi.org/10.3109/09553002.2014.937510
