QUANTITATIVE MORPHOLOGICAL ANALYSIS OF THE FEATURES REMODELING OF HEMOMICROCIRCULATORY BED OF THE TESTES AT THE ACTION ON THE BODY OF CADMIDIUM CHLORIDE
DOI:
https://doi.org/10.11603/1811-2471.2020.v.i2.11319Keywords:
testes, hemomicrocirculatory bed, morphometry, cadmium, chlorideAbstract
Modern researchers point to the negative impact of harmful environmental factors on the reproductive function in men. Functional and structural changes of the hemomicrocirculatory bed, which is the first to respond to harmful factors of the environment, have not been sufficiently studied in the testes.
The aim – to conduct a morphometric analysis of the features remodeling of the hemomicrocirculatory bed of the testes when exposed to the body of cadmium chloride.
Material and Methods. Morphologically there were studied the testes of 60 white male rats, which were divided into two groups. Group 1 included 30 intact animals, group 2 – 30 rats who were subcutaneously administered cadmium chloride at a dose of 6 mg/kg for 4 weeks. Euthanasia of the animals was performed by bloodletting under thiopental anesthesia a month after the start of the experiment. The hemomicrocirculatory bed of the testes was studied by filling their vessels with a mascara-gelatin mixture that was injected through the abdominal aorta. Enlightened and histological preparations stained with hematoxylin-eosin were made from the testes. The diameters of arterioles, precapillary arterioles, hemocapillaries, postpillary venules, venules, and the density of microvessels of the left and right testes were determined morphometrically. Quantitative indicators were processed statistically.
Results. Prolonged exposure to the body of cadmium chloride led to marked changes in the quantitative morphological parameters of the vessels of the hemomicrocirculatory bed of testes compared with control. The diameter of the arterioles of the left testis in the simulated experimental conditions decreased by 19.2 % (p<0.001), precapillary arterioles – by 23.3 % (p<0.001), hemocapillaries – by 16.7 % (p<0.001). The diameter of the postcapillary venules of the left testis thus increased by 32.7 %, venules – by 28.3 % (p<0.001), and the density of the microvessel decreased by 30.0 % (p<0.001), which indicated a deterioration of his blood supply.
The degree of remodeling of the microvasculature in the right testis was less pronounced. The diameter of arterioles of the right testis decreased by 18.2 %, precapillary arterioles – by 22.6 %, and hemocapillaries – by 15.8 % (p<0.001). The diameter of the postcapillary venules of the right testis increased by 31.5 %, venules by 27.8 % (p<0.001), and the density of microvessels decreased by 27.1 % (p<0.001). Morphological changes in structures dominated the left testis.
Conclusion. Prolonged action on the body of cadmium chloride leads to a pronounced remodeling of the vessels of the hemomicrocirculatory bed of the testes, which is characterized by pronounced its narrowing of the bringing (arterioles, precapillary arterioles), metabolic (hemocapillaries) units, enlargement of postcapillary venules and venules, venous plethora, hypoxia, dystrophic, necrobiotic changes of cells and tissues, infiltrative and sclerotic processes that dominated the left testis.
References
Avtandilov, G.G. (2002). Osnovy kolichestvennoy patologicheskoy anatomii [Basis of quantative pathological anatomy]. Moscow: Meditsyna [in Russian].
Bazalytska, S.V. (2016). Cholovicha neplidnist v Ukraini: osoblyvosti pato- i morphohenezu [Male infertility in Ukraine: features of pathogenesis and morphogenesis]. Kyiv: TOV “Chetverta khvylia” [in Ukrainian].
Hnatyuk, M.S., Konovalenko, S.O., & Tatarchuk, L.V. (2019). Osoblyvosti remodeliuvannia sudyn hemomikrotsyrkuliatornoho rusla yaiechok pry postrezektsiinii portalnii hipertenzii [Peculiarities of vascular remodeling of hematomicrocirculatory bаd at post-resection portal hypertension]. Shpytalna khirurhiya – Hospital Surgery, 4, 37-42 [in Ukranian].
Goralsky, L.P., Khomich, V.T., & Kononsky, O.I. (2011). Osnovy histolohichnoi tekhniky i morfofunktsionalni metody doslidzhen u normi i pry patolohii [Fundamentals of histological technique and morphofunctional methods of research in normal and pathology]. Zhytomyr: Polissia [in Ukrainian].
Dolynko, N.P. (2015). Vliyaniie ginko biloba na morfometricheskiye i funktsionalnye pokazateli spermatozoidov v usloviyakh khronicheskoy etanolovoy intoksikatsii [The influence of ginkgo biloba on the morphometric and functional indicator of spermatozoa in conditions of chronic ethanol intoxication].Vestnik Mogelevskoho derzhavnoho universytetu im. A.A. Kuliashova. Seriia Pryrodnychi nauky – Bulletin of Mogilev State University by A.A. Kuleshov. Natural Science Series, 2 (46), 99-102 [in Russian].
Dubinina, A.A. (2016). Toksychni rechovyny i metody yikh vyznachennia [Toxic substances and methods for their determination]. Kharkiv: KHDUKHT [in Ukrainian].
Konovalenko, S.O., Lototskyi, V.V., & Tatarchuk, L.V. (2016) Strukturni zminy v yaiechku pry dii na orhanism khlorydu kadmiiu [Structural changes in the testis when exposed to cadmium chloride]. Problemy viiskovoi okhorony zdorovia – Problems of Military Health Care, 45, 199-206 [in Ukranian].
Lapach, S.N., Gubenko, A.V., & Babych, P.N. (2001). Statisticheskye metody v mediko-biologicheskykh issledovanyiakh Excell [Statistical methods in biomedical research Excell]. Kyiv: Morion [in Russian].
Reznykov, O.H. (2003). Zahalni etychni pryntsypy eksperymentiv na tvarynakh [General ethical principles experiments on animals]. Endokrynolohiia – Endocrinology, 8, 1, 142–145 [in Ukrainian].
