ZINC OXIDE NANOPARTICLES ENHANCE OXIDATIVE AND NITRO-OXIDATIVE STRESS CAUSED BY HERBICIDE GLYPHOSATE
DOI:
https://doi.org/10.11603/1811-2471.2020.v.i2.11314Keywords:
nano-ZnO, glyphosate, oxidative and nitro-oxidative stressAbstract
The development of nanotechnologies contributes to the emergence new ultra high dispersed substance forms called nanomaterials which are widely used in scientific research, industry and medicine. The capability of nanoparticles to increase the transport of chemicals and drugs into cells and through the body barriers provides the possibility of the potentiating of chemical contaminants toxicity in case of their simultaneous intake into the organism.
The aim – to explore the effect of zinc oxide nanoparticles on the ability of herbicide glyphosate to cause oxidative and nitro-oxidative stress in blood serum and liver of experimental rats.
Material and Methods. Suspension of ZnO nanoparticles (0.5 ml) at a dose of 100 mg/kg and glyphosate (in the form of herbicide roundup) at a dose of 250 mg/kg of body weight were administered intragastrically to male rats for 14 days. The toxicants were administered simultaneously and separately. The total activity of NO-synthase, catalase, superoxide dismutase, content of NOx, thiobarbituric acid reactive substances, oxidative modified proteins, reduced glutathione, ceruloplasmin and total serum antioxidant activity were measured in serum and liver.
Results and Discussion. It was shown that zinc oxide nanoparticles affect negatively on the majority of studied parameters. The administration of glyphosate resulted in more profound changes of all indices. However, the maximal changes of the parameters were evidenced in the group of animals that were co-administered with zinc oxide nanoparticles and glyphosate. In that case, the content of thiobarbituric acid reactive substances, oxidative modified proteins, NOx and activity of NO-synthase and superoxide dismutase in rats were significantly worser compared with the similar indices in animals that were administered with the chemical toxicant only.
Conclusions. Zinc oxide nanoparticles enhance the capability of the herbicide glyphosate to cause oxidative and nitro-oxidative stress in blood serum and liver of the experimental rats.
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