MORPHOMETRIC EVALUATION OF PECULIARITIES REMODELING OF HEMOMICROCIRCULATORY BED OF THE TESTES AT THE ACTION ON THE BODY OF RUBOMYCIN HYDROCHLORIDE
DOI:
https://doi.org/10.11603/1811-2471.2019.v.i4.10793Keywords:
rubomycin hydrochloride, testes, hemomicrocirculatory bedAbstract
It is known that rubomycin – an antitumor antibiotic with pronounced cytostatic action is widely used in the clinic. The use of this drug agent in men can be complicated by azoospermia, in the morphogenesis of which the hemomicrocirculatory bed of the testes plays an important role.
The aim – to conduct morphometric analysis of remodeling features of the hemomicrocirculatory beds of the testes when exposed to rubomycin hydrochloride.
Materials and Methods. Morphometric study of the hemomicrocirculatory bed of the testes of 60 white male rats, which were divided into two groups. The group 1 consisted of 30 intact animals, group 2 – 30 rats, which were administered intraperitoneally with rubomycin hydrochloride at a dose of 30 mg/kg. Euthanasia of experimental animals was carried out by bloodletting of thiopental anesthesia a month after the beginning of the experiment.
The diameters of arterioles, precapillary arterioles, hemocapillaries, capillary venules, venules, and microvascular density per 1 mm² tissue of testes were morphometrically determined. Quantitative indicators were processed statistically.
Results and Discussion. It was established that the morphometric parameters of the microvessels of the hemomicrocirculatory bed of the testes under the influence on the body of rubomycin hydrochloride were changed significantly. Diameter of arterioles of the testes decreased by 17.0 %, precapillary arterioles – by 22.4 %, hemocapillaries – by 15.8 % (p<0.001), and the lumen of the capillary venules expanded by 31.1 %, venules – by 26.8 %, microvascular density decreased by 27.2 %. The revealed evidence of deterioration of blood supply of the investigated organ. The optically vast majority of the venous vessels of the hemomicrocirculatory bed are irregularly expanded, varicosally altered, with frequent sacculation, overflowing with blood cells, with the stasis, thrombosis, which indicated a pronounced violation of their drainage function. Venous congestion in the vessels studied led to an increase in hypoxia, dystrophy and necrosis of endothelial cells, myocytes, spermatogenic epitheliocytes, stromal structures, infiltrative and sclerotic processes.
Conclusions. Introduction into the body of experimental animals rubomycin hydrochloride leads to a pronounced remodeling of the blood vessels hemomicrocirculatory beds of the testes, which is characterized by a pronounced narrowing of the arterioles, precapillary arteriolts, hemocapillaries, and extensions of the postcapillary venules, venules, changes in angioarchitectonics of microvessels, venous plethora, hypoxia, dystrophy, necrobiosis of cells and tissues, infiltrative and sclerotic processes.
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