THE INFLUENCE OF DIFFERENT MODELS OF IMMOBILIZATIONAL STRESS ON MORPHOLOGICAL CHANGES OF HIGH AND LOW-RESISTANCE TO ACUTE HYPOXIC HYPOXIA RATS OF DIFFERENT SEX
DOI:
https://doi.org/10.11603/1811-2471.2019.v0.i2.10379Keywords:
stress, rats, resistance to hypoxia, heart, morphological changesAbstract
Stress is encountered daily in our lives. Everyone responds differently to one and the same stress, and does not think about its consequences for the body. Often, we do not take into account the damaging effects of stress on various organs, and in particular, the cardiovascular system. Determining the mechanisms of damaging the influence of stress on the heart in people with different reactivity can contribute to the development of individual methods of prevention and correction.
The aim of the study – to determine the effect of various modes of immobilization stress on morphological changes in the heart of rats of different sex with high and low resistance to hypoxia (HR, LR).
Material and Methods. In the experiment on 144 Wistar line rats (72 males, 72 females) morphological changes of heart were determined. The animals were divided into three groups – control and two experimental (who have under the immobilization stress). Stress was modeled 4 times by a one-hour immobilization of the rats on the back with an interval of 24 hours (stress 1) and 72 hours (stress 2). The studies were carried out 24 hours after the last immobilization.
Results. In a light-optical study of transverse sections of rats’ hearts, in all study groups of animals exposed to immobilization stress, minor damage to the myocardium was observed, the degree of which depended on the resistence to hypoxy, sex and the model of immobilization stress. In all groups of animals, moderate swelling of both cardiomyocytes and stroma, a different degree of necrobiosis of cardiomyocytes and microcirculation disorders were detected. In stress 1, compared with stress 2, the degree of myocardial damage was less. In HR animals, in comparison with LR, the damage was less pronounced. Major changes were noted for both types of stress in males.
Conclusions. Interval immobilization of rats leads to a violation of the morphological structure of the myocardium, the degree of development of which depends on resistance to hypoxia, sex and stress patterns. The greatest damage was observed in both types of stress in males.
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