ACTIVITY OF CYTOLYTIC PROCESSES IN RATS IN THE CONDITIONS OF CHRONIC NEOPLASTIC INTOXICATION AFTER APPLICATION OF CYTOSTATICS
DOI:
https://doi.org/10.11603/1811-2471.2019.v0.i2.10378Keywords:
1,2-dimethylhydrazine, cytolytic processes, cytostatics, erythrocyte intoxication index, aminotransferaseAbstract
Artificially induced tumors in certain carcinogens in laboratory animals provide an opportunity to study various aspects of carcinogenesis that cannot be effectively studied directly on the human body.
The aim of the study – to find out the dynamics of changes in cytological processes in the simulation of colon cancer by the introduction of 1,2-dimethylhydrazine hydrochloride and after applying cytostatic Xeloda.
Material and Methods. In rats, the features of the development of cytological processes in conditions of induced colon cancer, caused by the administration of 1,2-dimethylhydrazine and after the use of Xeloda cytostatics, were investigated. 1,2-dimethylhydrazine was administered subcutaneously at a dose of 7.2 mg/1 time per week for 30 weeks. Xeloda was administered intragastrically daily at a dose of 134 mg/kg of animal body weight for 21 days, starting immediately after a 7-month simulation of cancer process. The development of cytological processes was assessed by the activity of aminotransferases, alkaline phosphatase and erythrocyte intoxication index.
Results. Under conditions of DMH-induced intoxication, a significant progressive increase in the activities of aminotransferases and alkaline phosphatase in the blood serum of animals over 7 months was found, as well as an increase in the percentage of permeability of the red blood cell membranes. The use of Xeloda somewhat increased the activity of these enzymes and the erythrocyte index of intoxication, which indicates the absence of a positive effect of cytostatics on membrane-destructive processes in animals with non-plastic intoxication.
Сonclusion. The obtained results confirm the Xetoda cytostatic toxicity, which requires the introduction of additional corrective factors in order to eliminate its side effects on the liver and stabilize the permeability of cell membranes.
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