SAFETY ASSESSMENT OF INTRAHEPATIC INJECTION OF 10 % CALCIUM CHLORIDE SOLUTION IN WISTAR RATS: EXPERIMENTAL STUDY
DOI:
https://doi.org/10.11603/1811-2471.2019.v0.i2.10169Keywords:
10 % calcium chloride solution, intrahepatic administration, safety, complications, mortalityAbstract
The aim of experimental study was to evaluate the safety of intrahepatic administration of 10 % calcium chloride solution in vivo.
Material and Methods. The experiment was performed on 26 adult Wistar rats. Intrahepatic administration of 10 % calcium chloride was performed once. The dose was 5.0 mg/100.0 g of body weight of the animal. Average dose was (0.16±0.03) ml. There were evaluated: blood count test, liver function tests, ionized calcium concentration, specific complications, mortality. The follow-up time: 1, 3, 5, day for blood count test and liver samples and 1, 5, 10 minutes for ionized calcium. Complications and mortality were assessed up to 21 days.
Results. On the 1st and 3rd day, changes in the parameters of general blood count and liver tests were characterized by a decreasing of hemoglobin rates (p=0.004) and increasing of leukocytes count (p=0.005), levels of total bilirubin (p=0.005), ALT (p=0.005), AST (p=0.005), alkaline phosphatase (p=0.005) as compared to baseline. On the 5th day, these parameters were normalized to baseline: hemoglobin (p=0.366), leukocytes (p=0.051), total bilirubin (p=0.469), ALT (p=0.575), AST (p=0.959), alkaline phosphatase ( p=0.169), respectively. Increasing of ionized calcium concentration in the rats blood serum in the first minute compared with the initial (p=0.005) was not accompanied by fatal consequences. Changes in all hematological parameters occurred within the referent rates and were not accompanied by clinical manifestations. Postoperative complications and mortality were not associated with intrahepatic administration of the drug.
Conclusion. Intrahepatic administration of 10 % calcium chloride solution at a dose of 5.0/100.0 mg of body weight of an animal is not accompanied by impaired liver function, homeostasis of blood calcium ions, the development of local complications and mortality.
References
Forner, A., Reig, M., & Bruix, J. (2018). Hepatocellular carcinoma. Lancet, 391, 1301-1314. Retrieved from: https://doi.org/10.1016/S0140-6736(18)30010-2. DOI: https://doi.org/10.1016/S0140-6736(18)30010-2
Balogh, J., Victor 3rd, D., Asham, E.H., Burroughs, S.G., Boktour, M., Saharia, A., ... & Monsour Jr., H.P. (2016). Hepatocellular carcinoma: a review. J. Hepatocell Carcinoma, 3, 41-53. Retrieved from: doi:10.2147/JHC.S61146. DOI: https://doi.org/10.2147/JHC.S61146
Van Cutsem, E., Cervantes, A., Nordlinger, B., & Arnold, D. (2014). Metastatic colorectal cancer: ESMO Clinical Practice Guidelines for diagnosis, treatment and follow-up. Ann. Oncol., 3, 1-9. Retrieved from: doi: 10.1093/annonc/mdu260. DOI: https://doi.org/10.1093/annonc/mdu260
Fitzgerald, T.L., Brinkley, J., Banks, S., Vohra, N., Englert, Z.P., & Zervos, E.E. (2014). The benefits of liver resection for non-colorectal, non-neuroendocrine liver metastases: a systematic review. Langenbecks Arch. Surg., 399, 989-1000. DOI: https://doi.org/10.1007/s00423-014-1241-3
Ryan, M.J., Willatt, J., Majdalany, B.S., Kielar, A.Z., Chong, S., Ruma, J.A., & Pandya, A. (2016) Ablation techniques for primary and metastatic liver tumors. World J. Hepatol., 8 (3), 191-199. Retrieved from: http://dx.doi.org/10.4254/wjh.v8.i3.191. DOI: https://doi.org/10.4254/wjh.v8.i3.191
Dronov, A.I., Kovalskaya, I.A., Homenko, D.I., Zharkov, A.Ya., Leshchenko, V.M., Krutko, O.A., & Kozachuk, Ye.S. (2018). Termometriia protsessa lokalnogo kriovozdeystviya v biologicheskoy tkani na diskretnykh glubinakh: razrabotka kompleksa izmeritelnogo intraoperatsionnogo termoparnogo chetyrehkanalnogo (KIIT-4) [Thermometry of the process of local cryoinfluence in biological tissue at discrete depths: development of a complex of measuring intraoperative four-channel thermocouple (KIIT-4)]. Khirurgiya. Vostochnaya Yevropa – Surgery. Eastern Europe, 7 (1), 21-27 [in Russian].
Baust, J.G., Gage, A.A., Johansen, T.E. B., & Baust, J.M. (2014). Mechanisms of cryoablation: Clinical consequences on malignant tumors. Cryobiology, 68 (1), 1-11. doi:10.1016/j.cryobiol.2013.11.001. DOI: https://doi.org/10.1016/j.cryobiol.2013.11.001
Diachenko, M.I., Klymenko, M.M., Domanskyi, S.V., Sankin, Yu.Yu., & Nechaiev, I.H. (2016). Problemy ta perspektyvy vykorystannia kriotekhnolohii v neiroonkolohii [Problems and prospects of using cryotechnologies in neuroconclusion]. Molodyi vchenyi – Young Scientist, 12 (39), 303-306 [in Ukrainian].
Dronov, O.I., Khomenko, D.I., Bakunets, P.P., & Teterina, V.V. (2017). Temperaturni pokaznyky pislia kriovplyvu, potentsiiovanoho dystyliovanoiu vodoiu, na eksperymentalnii modeli pechinky svyni za umov vidsutnosti splankhnichnoho krovotoku [Temperature changes during cryoeffect potentiated with distilled water assessed in porcine liver model without splanchnic blood flow]. Probl. Cryobiol Cryomed., 27 (4), 348-355 [in Ukrainian]. doi: 10.15407/cryo27.04.348. DOI: https://doi.org/10.15407/cryo27.04.348
Aydınцz, S., Sleymanoğlu, S., Haholu, A., Uzun, G., Karademir, F., Yıldız, Ş., & İsmail, S. (2007). Hyperbaric oxygen therapy in calcium chloride extravasation injuries: An experimental animal studyeur. J. Gen. Med., 4 (4), 186-189. DOI: https://doi.org/10.29333/ejgm/82527
Normatyvno-dyrektyvni dokumenty MOZ Ukrainy. Instruktsiia dlia medychnoho zastosuvannia preparatu kaltsiiu khloryd [Regulatory documents of the Ministry of Health of Ukraine. Instructions for medical use of calcium chloride]. Retrieved from: http://mozdocs.kiev.ua/likiview.php?id=19297 [in Ukrainian].
