FEATURES OF NON-SPECIFIC AND SPECIFIC LINKS OF IMMUNE PROTECTION IN RATS WITH ACUTE PERIODONTITIS WITH HYPOTHYRODISM

Authors

  • T. I. Dzetsiukh I. Horbachevsky Ternopil State Medical University

DOI:

https://doi.org/10.11603/1811-2471.2019.v0.i1.10050

Keywords:

periodontal inflammation, hypothyroidism, non-specific and specific parts of the immune defense

Abstract

To study the effect of experimental hypothyroidism on the state of nonspecific and specific immune defense in rats with acute soft tissue injury of the gums, we determined the NBT test, indicators of the cellular component of the immune system (CD4, CD8), and studied the content of IgG, IgM and IgA in serum StatFax (USA).

The aim – to study the features of non-specific and specific parts of the immune defense in rats with acute periodontitis with hypothyroidism.

Material and Methods. Hypothyroidism in rats was caused by the administration of mercazolil at a dose of 25 mg/kg for 21 days. Control was carried out on the level of thyroxin, triiodothyronine and thyroid-stimulating hormone, as well as on the mass of animals and their physical activity. The comparison group included animals that were not used by Mercazolil. The effect of hypothyroidism on the course of the inflammatory process in periodontitis was studied on the model of inflammation caused by acute injury of the soft tissues of the gums. Under the thiopental anesthesia (30 mg/kg) on the labial side, the working head of the ultrasonic generator – radiator from the ART ultrasonic scaler (US) was brought to the periodontal tissues of the lower incisor, and a single directional influence of ultrasonic frequency oscillations was performed for 60 s with the following exposure parameters: the oscillation frequency is 50 kHz, the radiation power is 1.2 W ∙ cm² with an exposure exposure of 60 s. The operation was performed on the fourteenth day after the first injection of mercazolil. After 1 and 8 days after surgery, rats were decapitated under thiopental anesthesia (50 mg/kg). The comparison groups were animals with experimental hypothyroidism and rats with acute mechanical injury of the soft tissues of the gums. Control served material from intact animals.

Results and Discussion. Inflammation in rats with acute gum injury on the background of hypothyroidism led to an increase in the content of active neutrophils in peripheral blood, a decrease in CD-4 lymphocytes, a violation of the ratio of the main lymphocyte subpopulations (CD-4 and CD-8), accompanied by a significant decrease in immunoregulatory index, inhibition of secretion IgG, IgM and IgA at the system level.

Conclusions. In euthyroid rats, the normal course of the inflammatory process is observed with a predominance of T-helper growth over cytotoxic T-lymphocytes and, accordingly, an increase in immunoregulatory index. A deficiency in the cellular level of the immune response was found to reflect the reinforcement of the mutual negative effects of the inflammatory process in the periodontium and systemic disorders of the immune defense in conditions of a concomitant thyroid hormone deficiency. When simulating acute periodontitis on the background of hypothyroidism, depression of the humoral component of the immune system was noted already on the first day, which was characterized by a significant decrease in immunoglobulins of all classes.

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Published

2019-04-23

How to Cite

Dzetsiukh, T. I. (2019). FEATURES OF NON-SPECIFIC AND SPECIFIC LINKS OF IMMUNE PROTECTION IN RATS WITH ACUTE PERIODONTITIS WITH HYPOTHYRODISM. Achievements of Clinical and Experimental Medicine, (1), 58–67. https://doi.org/10.11603/1811-2471.2019.v0.i1.10050

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Section

Original research articles