MECHANISMS OF IMMOBILIZATION STRESS ADAPTATION IN LOW-RESISTANT TO HYPOXIA RATS OF DIFFERENT GENDER
DOI:
https://doi.org/10.11603/1811-2471.2019.v0.i1.10048Keywords:
immobilization stress; rats, resistance to hypoxia, antioxidant system, cardiointervalographyAbstract
Hypoxia is one of the leading factors in development of many diseases. Hypodynamia also leads to development of diseases of cardiovascular system. Identification of mechanisms that provide adaptation to sedentary lifestyle in people with different reactivity can contribute to development of individual methods of prevention and correction of identified violations.
The aim of the study – to determine parameters of the antioxidant system and condition of the autonomous nervous system in rats of different gender with a low-resistance to hypoxia immobilization stress.
Material and Methods. Stress was modeled 4 times by an hour-long immobilization of rats back down with an interval each 24 hours. In the heart, the activity of superoxide dismutase (SOD), catalase, the concentration of reduced glutathione, activity of glutathione peroxidase (GP), glutathione reductase (GR), and peroxidase activity in blood were determined. Animals underwent cardiointervalography.
Results. In intact male rats, compared with females, there was higher activity of antioxidants. Under stress we noted the growth of SOD and catalase, decreased peroxidase activity of blood in animals, regardless of sex. Detected changes of activity of enzymes were significantly higher in females. Under stress we noted the growth of reduced glutathione, decreased GP and GH, and in females – reduction of reduced glutathione, increase in GP and GR. In cardiointervalography study was found that in intact males, higher adrenergic effects on the heart. Under stress decreased sympathetic effects in all rats, in males reduce regulatory effects on the heart, and in females, the allocation of adrenaline is increased by humoral channels.
Conclusions. In intact low-resistance to hypoxia male rats, compared with females, higher antioxidant activity, which may be due to a higher level of metabolic processes in the heart. Interval immobilization of low-resistance to hypoxia rats leads to activation of antioxidants, which is more pronounced in females. In intact males adrenergic effects on the heart prevail. Interval immobilization of low-resistance to hypoxia rats leads to decrease tone of the sympathetic portion of the autonomic nervous system, which has an oxygen-retaining effect, causes the reduction of regulatory mechanisms on the heart only in males.
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