CHANGES IN THE GLUTATHIONE SYSTEM'S ACTIVITY OF INTERNAL ORGANS IN THE FIRST HOURS OF EXPERIMENTAL LIMB ISCHEMIA-REPERFUSION SYNDROME, COMBINED WITH BLOOD LOSS AND MECHANICAL INJURY
DOI:
https://doi.org/10.11603/1811-2471.2019.v0.i1.10043Keywords:
ischemia-reperfusion, tourniquet, bleeding, experiment, ischemia, mechanical trauma, glutathione systemAbstract
Blood loss of various genesis irreversibly leads to hypoxia, which in turn triggers the activation of lipid peroxidation and, as a result, cell membranes are damaged. The use of a hemostatic tourniquet, which can complicate the course of traumatic illness under these pathological conditions, has not been fully studied.
The aim of the study – to investigate the features of the response of glutathione system's enzymes of internal organs on the pathogenetic stimulation from modifications of ischemic-reperfusion syndrome in the first hours after its application.
Materials and methods. The determination of the content of glutathione peroxidase (GP), glutathione reductase (GR) and reduced glutathione (RG) in homogenates of internal organs of rats on the base of experimental acute ischemia-reperfusion (IR); IR, associated with mechanical trauma (MT); IR, combined with blood loss (IR+B), and IR combined with blood loss and mechanical trauma (IR+MT) and comparison of results with only blood loss. The acquired exponents are processed statistically.
Results and Discussion. It has been experimentally established that the greatest reduction in the activity of the glutathione system's enzymes – its exhaustion – occurs on the background of ischemia-reperfusion syndrome, combined with blood loss and mechanical trauma of the limb. Already in the first hours in the liver, kidney, heart and lung, a decrease of recovered glutathione was found at 28.8, 30.6, 31.0 and 19.8 times reteably.
Conclusions. An active response for all of examined parameters was found. Towards the activity of enzymes, the following pattern was established: in the first hours, the isolated overlay of the tourniquet led to compensatory changes. The tourniquet, combined with blood loss, has reduced the activity slightly lower than the blood loss itself, which in our opinion, can be explained by the fact that a large circle of blood flow has received a significant concentration of pathogenic factors – due to ischemia, but at the first hour they still managed to evoke, obviously only local damage, whereas hypoxic processes activated by proper blood loss occurred on its background at the system level.
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