EFFICIENCY OF EXOGENOUS MELATONIN USE IN TUBAL INFERTILITY
DOI:
https://doi.org/10.11603/2415-8798.2019.1.9956Keywords:
tuboperitoneal infertility, melatonin, infertility treatment, antioxidant therapyAbstract
Tubal-peritoneal infertility is a common factor in female infertility, accompanied by a disruption in the pro/antioxidant system. Melatonin is a powerful neutralizer of free radicals, so the use of exogenous melatonin, as an antioxidant therapy for such infertility, is quite reasonable.
The aim of the study – to determine the effectiveness of antioxidant therapy with exogenous melatonin in infertility of tubal origin.
Materials and Methods. The study involved 65 women with tubal-peritoneal infertility who were treated using assisted reproductive technology. Depending on the desire of the woman to receive different treatment, the patients were divided into 2 groups. The group 1 included 33 patients who received exogenous melatonin, and the group 2 included 32 women who were treated according to the standard therapy. The control group consisted of 35 practically healthy fertile women with realized reproductive function. The level of endogenous melatonin, oxidative protein modification, the content of TBA-active products, the activity of enzymes superoxide dismutase (SOD) and catalase, the level of fragmented DNA (f-DNA) was determined for all women.
Results and Discussion. The determination of endogenous melatonin in serum showed a statistically lower level of hormone in women with infertility, compared with control group. After receiving melatonin for three months, the level of lipid peroxidation and oxidative modification of proteins decreased, while the activity of antioxidants in SOD and catalase increased both in the endometrium and in the follicular fluid. Correlation analysis between the indicators of the antioxidant system, the level of f-DNA in the endometrium and the follicular fluid in tubal-peritoneal infertility showed a significant effect of exogenous melatonin on the normalization of the free radical process, which confirms its positive role in the regulation of oxidative stress (directly or indirectly) and apoptosis.
Conclusions. Antioxidant therapy with exogenous melatonin for three months is enough for the effective activation of endogenous antioxidant defense mechanisms in the body, which leads to a decrease in the content of free radicals and the normalization of apoptosis in the reproductive tract of women.
References
Claustrat, B., & Leston, J. (2015). Melatonin: Physiological effects in humans. Neurochirurgie, 61(2-3), 77-84.
Nayki, C., Nayki, U., Gunay, M., Kulhan, M., Çankaya, M., Humeyra Taskın Kafa, A., & Balci, G. (2017). Oxidative and antioxidative status in the endometrium of patients with benign gynecological disorders. J. Gynecol. Obstet. Hum. Reprod, 46 (3), 243-247.
Orlova, V.V. (2018). Vyvchennia stanu pro/antyoksydantnoi systemy v reproduktyvnomu trakti zhinok z trubno-perytonealnym bezpliddiam [Oxidative stress biomarkers in reproductive tract of women with tuboperitoneal infertility]. Medycyna sohodni i zavtra – Medicine today and tomorrow, 1 (78), 72 [in Ukrainian].
Dubinina, E., Burmistrov, S., Xodov, D., & Porotov, I. (1995). Okislitelnaya modifikatsiya belkov syvorotki krovi cheloveka, metod yeye opredeleniya [Oxidative modification of human serum protein, method of its determination]. Voprosy meditsinskoy khimii – Questions of Medical Chemistry, 1 (41), 24-26 [in Russian].
Orekhovіch, V.N. (1977). Modern methods in biochemistry [Modern methods in biochemistry]. Moskov: Medicina, 66-68 [in Russian].
Chevary, S., Chaba, Y., & Sekej, J. (1985). Rol superoksiddismutazy v okislitelnykh protsessakh kletki i metod opredeleniya yeye v biologicheskikh materialakh [The role of superoxide dismutase in the oxidative processes of the cell and the method for its determination in biological materials]. Labor. Delo – Labor. matter, 11, 678-681 [in Russian].
Koroliuk, M.A., Ivanova, L.I., Majorova, I.G., Tokarev, V.E. (1988). Metod opredeleniya aktivnosti katalazy [Method for determining of catalase activity]. Labor. Delo – Labor. matter, 1, 16-18 [in Russian].
Burton, K. (1956). A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem. 62, 315-323.
Barberino, R.S., Menezes, V.G., Ribeiro, A.E.A.S., Palheta, R.C.Jr., Jiang, X., Smitz, J.E.J., & Matos, M.H.T. (2017). Melatonin protects against cisplatin-induced ovarian damage in mice via the MT1 receptor and antioxidant activity. Biol Reprod., 96 (6), 1244-1255.
Sinha, K., Das, J., Pal, P.B., & Sil, P.C. (2013). Oxidative stress: the mitochondria-dependent and mitochondria-independent pathways of apoptosis. Archives of Toxicology, 87 (7), 1157-1180.
Solovjova, N.V. (2015). Antyoksydantna aktyvnist melatoninu i hlutationu na osnovi porivnialnoho analizu rezultativ kvantovokhimichnykh ta elektrokhimichnykh doslidzhen [Antioxidant activity of melatonin and glutathione based on comparative analysis of the results of quantum chemical and electrochemical studies]. Visnyk VDNZU “Ukrayinska medychna stomatolohichna akademiia” – Bulletin HSEEU “Academy of Medical Dentistry of Ukraine”, (49), 190-194 [in Ukrainian].
Downloads
Published
How to Cite
Issue
Section
License
Authors who sent their manuscript to "Вісник наукових досліджень. Bulletin of Scientific Research" Surgery agree to the following terms:
a. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal. 
b. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
c. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access)
