MORPHOFUNCTIONAL CHANGES IN CELL CULTURES OF THYROID GLAND OF NEWBORNE RATS IRRADIATED in utero BY RADIO-IZOTOPES OF IODINE-131 IN VARIOUS TERMS OF GESTATION

Authors

  • O. A. Boyko 1State institution "National Scientific Center of Radiation Medicine of the National Academy of Medical Sciences of Ukraine
  • G. Y. Lavrenchuk State institution "National Scientific Center of Radiation Medicine of the National Academy of Medical Sciences of Ukraine
  • V. R. Gurando Uzhgorod National University
  • A. I. Dovgalyuk State Pedagogical University "I. Gorbachevsky Ternopil State Medical University, Ministry of Health of Ukraine"
  • I. M. Klishch I. Horbachevsky Ternopil State Medical University

DOI:

https://doi.org/10.11603/2415-8798.2019.1.9954

Keywords:

thyroid gland, 131I, prenatal irradiation, cell culture, micronucleus, apoptosis.

Abstract

SUMMARY. Human organism is especially sensitive to radiation exposure due to incorporated radioisotopes of iodine in the early stages of ontogenesis (prenatal and early postnatal). Period between the 9th and 40th days after conception is considered to be especially crucial. Among the affected contingents of the Chornobyl catastrophe, which were most exposed to radioactive 131I, it was revealed that according to remote medical consequences the vulnerable individuals were not the only who irradiated in childhood and adolescence, but also those irradiated in utero in the first and third trimesters of the gestation.

The aim of our investigation was to find out the morphofunctional changes in the primary culture of the thyroid gland of newborn rats irradiated in utero by  131I  radioisotope in different gestational periods.

Materials and Methods.  To reach the aim the primary cell cultures from the thyroid gland tissues of newborn rats were obtained and cultivated, and also cytological, biophysical, statistical methods were used.

Results. Morphofunctional changes in the primary culture of thyroid cells of newborn rats were evaluated. Cytotoxicity and mutagenicity of intrauterine influence of 131I with activity of 27.35 kBq per pregnant female as a result of the effect of radioisotope in different gestational periods on the 12th, 13-th and the 14th day were established. The doses for the fetus thyroid gland were 0.19 ± 0.05, 0.37 ± 0.06, 1.44 ± 0.09 Gy, respectively.

Conclusions. A comparative morphofunctional characteristic of the primary culture of the thyroid gland of the newborn rats obtained from intact animals and under the influence of 131I radioisotope in different gestational periods was given. It has been shown that the effect of 131I in utero leads to the thyroid gland changes at the cellular level (binucleated cells and cells with micronuclei, apoptosis and disturbances of follicular formation). Тhe presence of "ring-like" cells is an indicator of the genome instability and a sign of neoplastic transformation.

Author Biographies

G. Y. Lavrenchuk, State institution "National Scientific Center of Radiation Medicine of the National Academy of Medical Sciences of Ukraine

Lavrenchuk Galina Yosypivna – Laboratory of Cellular Radiobiology, Institute of Experimental Radiology, State Institution "National Research Center for Radiation Medicine AMS Ukraine"; 53 Melnikova, Kyiv, Ukraine, 04050, e-mail: hl20071956@ukr.net; tel.: +0676043659

 

V. R. Gurando, Uzhgorod National University

Gurando Viacheslav Radomirovich – PhD, Assistant professor, prosthesis dentistry department, Uzhorod National University, 14 Uzhgorod street, Uzhgorod, Ukraine, 88000 mail: radodent@gmail.com, tel.: +380966104524

A. I. Dovgalyuk, State Pedagogical University "I. Gorbachevsky Ternopil State Medical University, Ministry of Health of Ukraine"

Dovgalyuk Alina Ivanivna - PhD, Associate professor of the Histology and Embryology department, I. Gorbachevsky Ternopil State Medical University, 12 Ruska street, Ternopil, Ukraine, 46000, e-mail: dovgalyuk@tdmu.edu.ua,  tel: +380676736785

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Published

2019-04-10

How to Cite

Boyko, O. A., Lavrenchuk, G. Y., Gurando, V. R., Dovgalyuk, A. I., & Klishch, I. M. (2019). MORPHOFUNCTIONAL CHANGES IN CELL CULTURES OF THYROID GLAND OF NEWBORNE RATS IRRADIATED in utero BY RADIO-IZOTOPES OF IODINE-131 IN VARIOUS TERMS OF GESTATION. Bulletin of Scientific Research, (1), 103–111. https://doi.org/10.11603/2415-8798.2019.1.9954

Issue

No. 1 (2019)

Section

EXPERIMENTAL RESEARCH

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