INFLUENCE OF EXPERIMENTAL TOURNICET ON INTENSITY OF PEROXIDATION OF LIPIDS IN INTERNAL ORGANS OF RATS

Abstract

Bleeding (B), which is further complicated by the ischemic effect of the hemostatic tourniquet (T), activates lipid peroxidation (LPO). The latter, under normal conditions without systemic pathological effects, may gain scope, creating preconditions for triggering the syndrome of the systemic inflammatory response and the development of multiple organ failure syndrome.

The aim of the study – to learn the state of LPO in internal organs such as the liver, kidneys, lungs and heart in the pathogenesis of experimental ischemia-reperfusion (EIR) in the period of acute response to bleeding and the period of early manifestations of traumatic illness and comparison with changes occurring on the background of isolated ischemia-reperfusion (T).

Materials and methods. 140 male white rats, which were divided into 3 groups, were the object of the study. Animals of the control group were withdrawn from the experiment without surgical intervention, whereas in the experimental groups, it was simulated: the application of a hemostatic tourniquet on the thigh (T) for two hours; isolated blood loss from the femoral vein (40 %) – (B); two-hour ischemia associated with blood loss (T + B). The slaughter was have done at 1, 3, 7 and 14 days after the injury.

Results and Discussion. All types of experimental interventions – isolated hemorrhage, turnstile and bleeding, combined with a hemostatic tourniquet, caused reliable on the indices of activation of LP in all investigated internal organs due to hyperproduction of malonic dialdehyde (MDA) of diene and triene conjugates.

Conclusions. The results of the experiments revealed significant activation of lipid peroxidation – in the liver, and somewhat slower - in the kidneys, heart and lungs. At the same time, the isolated overlay of the hemostatic tourniquet triggered the activation of the LPO, most pronounced, similarly, in the liver. Indicators remained significantly higher compared to baseline levels even at 14 days, although not so pronounced as on the background of isolated bleeding and bleeding combined with a tourniquet.