INFLUENCE OF EXPERIMENTAL MATERNAL HYPO- AND HYPERTHYROIDISM ON THE LECTIN PROFILE OF PROGENY ADRENAL GLANDS

Abstract

By means of lectin histochemistry methods, the influence of experimental hypo- and hyperthyroidism of maternal organism on the structural components of progeny adrenal glands has been investigated on the successive stages of pre- and postnatal ontogenesis.

The aim of the study – to expand the spectrum of known carbohydrate determinants to obtain a more complete picture of glycemic adrenal gland changes that developed under conditions of simulated experimental pathology.

Material and Methods. Hypothyroidism was induced by daily feeding of female rats with 10 mg/kg of anti-thyroid drug Mercacolyl, and hyperthyroidism was induced by feeding L-thyroxine at a dose of 100 mkg/kg. The lectin panel included concanavalin A (Con A), Lens culinaris agglutinin (LCA), Narcissus pseudonarcissus agglutinin (NPA), peanut agglutinin (PNA), Helix pomatia agglutinin (HPA), Clitocybe nebularis fungus agglutinin (CNFA), wheat germ agglutinin (WGA), Sambucus nigra agglutinin (SNA), Laburnum anagyroides bark agglutinin (LABA).

Results and Discussion. It was estimated that the morphogenesis of rat adrenal glands basically culminates on the 10th postnatal day and is manifested by the selective exposure of LCA, PNA, CNFA, ​​WGA, SNA and LABA lectin receptor sites in the glandular medullary portion. Hypothyroidism of the maternal organism caused delayed maturation, dilation of medullary vasculature, swelling of the parenchyma in combination with leukocyte infiltration of zona reticularis and medullary portion, alterations in glycomic structure of progeny adrenal glands. Changes in the glycoreceptors induced by hypo- and hyperthyroidism were similar yet not identical, which may be due to the negative effect of both pathological conditions on the final steps of glycosylation. WGA can be used for differential detection of epinephrocytes and norepinephrocytes, apoptosomes in the adrenal gland medulla; PNA – for visualization of Golgi complex in within the adrenocorticocytes, and secretory granules of chromaffin cells; LCA and LABA – for the detection of Golgi complex in chromaffin cells; NPA and PNA – for selective labeling of activated macrophages; Con A and SNA – for labeling of migrant eosinophilic granulocytes, which under conditions of hypothyroidism? But not hyperthyroidism infiltrated stroma of the adrenal glands.

Conclusions. Current study showed a significant impact of thyroid hormones imbalance within the maternal organism on morphogenesis, micromorphology, and carbohydrate determinants of progeny adrenal glands, with hypothyroidism inducing more pronounced changes in comparison with hyperthyroidism. The obtained data on the possibilities of selective marking by lectins of the rat adrenal glands structural components may be useful for monitoring of physiological and pathological processes in these same organs.