FEATURES OF LIPIDS PERIPHERAL OXIDATION AND ANTIOXIDANT PROTECTION DISORDERS IN BLOOD IN THE DYNAMICS UNDER IMMOBILIZATIONAL STRESS DEVELOPMENT DURING ADRENALIN MIOCARDIAL DAMAGE

Abstract

Stress reactions of the human body and animals are one of the most important problems of the present time, the processes of adaptation to which are actively explored. Acute epinephrine myocardial damage causes heart disease disorder, which manifests itself in lowering of its suppressive function, which leads to a decrease in cardiac output.

The aim of the study – to find out the peculiarities of changes of lipid peroxidation and antioxidant blood levels in rats under conditions of development of immobilization stress and adrenal damage of myocardium.

Мaterials and Methods. The experiments were carried out on white male rats of the Vistar line weighing 180–200 g, which were divided into 4 groups 10 animals in each (one control and three tested). The state of the processes of lipid peroxidation (LPO) was assessed by the content of new conjugates and malonic dialdehyde in the blood, which were determined at different periods (1^st, 3^rd, and 5^th day) after the IP in rats. Antioxidant protection (AOP) was evaluated by activity of catalase and superoxide dismutase enzymes.

Results and Discussion. Complex biochemical studies of lipid peroxidation and antioxidant system in animals of different groups (intact, immobilization stress, adrenal damage of myocardium, separately and in their combination) showed that consistently excessive formation of products of lipoperoxidation occurs on the background of decrease in activity at all stages catalase and superoxide dismutase formation (1^st, 3^rd, and 5^th day) in the blood, indicating the development of oxidative stress.

Сonclusions. Consequently, the experiment conducted allowed to establish a significant violation of the prooxidant balance in animals under conditions of immobilization stress and adrenal damage to the myocardium.