CHANGES OF BLOOD SERUM CYTOKINE PROFILE IN RATS IN RESPONSE TO COMBINED INTRODUCTION OF SILICON DIOXIDE NANOPARTICLES AND CHEMICAL TOXICANT LEAD ACETATE
DOI:
https://doi.org/10.11603/2415-8798.2017.3.8093Keywords:
nanoparticles, silicon dioxide, lead acetate, cytokines.Abstract
Summary. The established capability of nanoparticles to intensify the transport of chemicals and drugs into cells and across the body barriers makes the possibility of potentiating the toxic effects of chemical contaminants in case of their combined introduction into the body with nanoparticles.
The aim of the study – to evaluate the integral effect of silicon dioxide nanoparticles and chemical toxicant lead acetate on blood serum cytokine profile. Materials and Methods. The experiments were conducted on 40 white outbred male rats, which were divided into 4 groups. The animals of the group 1 (control) were daily administered intragastrically with normal saline. The rats of the group 2 were introduced with colloidal solution of silicon dioxide nanoparticles in a dose of 50 mg/kg of body weight. The animals of the group 3 were injected with aqueous lead acetate at a dose of 20 mg/kg of body weight (on a lead basis); and the rats of the group 4 were administered daily with a solution of silicon dioxide nanoparticles and lead acetate during 3 weeks at the abovementioned doses. Concentrations of cytokines TNF-α, IL-1β, IL-6, IL-4, IL-10 were measured in blood serum of the rats by means of ELISA test.
Results and Discussion. It was established that silicon dioxide nanoparticles did not change significantly the studied indices. In blood serum of the rats administered with lead acetate, a significant increase in the concentration of proinflammatory cytokines TNF-α, IL- 1β, IL-6 and a decrease in the level of anti-inflammatory cytokines IL-4 and IL-10 were determined. The cytokine profile has been changed the most significantly in blood serum of the animals administrated with both nanoparticles of silicon dioxide and lead acetate. In this case, the concentration of TNF-α, IL-1β and IL-6 was significantly increased by 27, 18.8 and 36.9 % (p<0.05) respectively, and the content of IL-4 and IL-10 was significantly decreased by 25.8 and 24.7 % (p><0.05) respectively, compared to the group of animals administered with chemical toxicant only.
Conclusions. Silicon dioxide nanoparticles intensify the capability of chemical toxicant lead acetate to increase proinflammatory cytokines output and reduce the output of anti-inflammatory cytokines.>
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