CORRECTION OF METABOLIC DISORDERS DURING CONSERVATIVE TREATMENT OF ECTOPIC PREGNANCY
DOI:
https://doi.org/10.11603/2415-8798.2017.3.8085Keywords:
methotrexate, acid phosphatase, alkaline phosphatase, calcium folio.Abstract
According to epidemiological studies in economically developed countries, the frequency of ectopic pregnancies (EP ) is 1.2–2.0 % of the total number of pregnancies and 0.8–2.4 % of births and is 4th among the causes of maternal mortality.
The aim of the study – to investigate the toxic effects of methotrexate on experimental animals, depending on the dose, and assess the protective effect of calcium folinate after single and double administration of methotrexate.
Materials and Methods. The work was performed on 54 laboratory white rats, which were kept under standard vivarium conditions. Selected sexually mature non-pregnant females with body weight (220 ± 5) g. All manipulations with experimental animals were conducted in accordance with the rules of the European Convention for the Protection of Vertebrate Animals used for experimental and other scientific purposes (Strasbourg, 1986). Experimental animals were divided into 9 groups of 6 animals.
Results and Discussion. We examined the endogenous intoxication index in 54 experimental rats against the background of single and double administration of methotrexate at a dose of 1 mg / kg at 3 and 7 days. The protective effect of calcium folinate in a dose of 0.1 mg / kg, which was administered 24 hours after the injection of methotrexate, was investigated. This experiment showed an increase in the level of acidic and alkaline phosphatases in rats when intramuscular administration of methotrexate during ectopic pregnancy. The use of calcium folinate reduces the side effect of the main drug, which is shown by a significant difference between experimental groups of rats.
Conclusions. This experiment showed an increase in the level of acidic and alkaline phosphatases in rats when intramuscular administration of methotrexate during ectopic pregnancy. Correction of metabolic disorders in the conservative treatment of ectopic pregnancy may be carried out by an antidote of methotrexate – calcium folinate.
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