METABOLIC AND STRUCTURAL MYOCARDIAL DISEASE IN THE CONDITIONS OF EXPERIMENTAL ACUTE LUNG INJURY
DOI:
https://doi.org/10.11603/2415-8798.2017.3.8076Keywords:
acute lung injury, heart, lipid peroxidation, antioxidant defense, morphological disorders.Abstract
The study of metabolic and structural myocardial disorders in conditions of experimental acute lung injury allows revealing the pathogenesis of processes and predicting the peculiarities of the course in this pathology.
The aim of the study – to find out the dynamic indicators of peroxide lipid oxidation, antioxidant protection and pathomorphological changes in the heart under conditions of experimental state lung injury.
Materials and Methods. Experiments were conducted on white nonlinear male-rats weighing 200–220 g. Modeling of acute lung injury was performed according to the method of G.Matute-Bello. Animals were excised from the experiment at 12, 24, 48 and 72 hours after the simulation of acute lung injury. To study morphological changes, myocardial biopsies were used, the sections of which were stained with hematoxylin and eosin. In the heart homogenate established indicators of lipid peroxidation and antioxidant protection.
Results and Discussion. In the acute lung injury due to intracutaneous administration of chloride acid, the processes of lipid peroxidation are activated in the heart, as evidenced by the accumulation of TBA-active products of the LPO, with a maximum of 24 hours (by 74.7 % compared with the control, p <0.05). Under these conditions, the indexes of the enzymatic level of antioxidant defense with a compensatory increase above the control level of SOD in 48 hours (by 88.3 %, p <0.05), catalase after 72 hours (by 63.5 %, p <0.05). Under the conditions of modeling of acute lung injury, the structure of myocardial damage with uneven blood filling of blood vessels in 12 hours of observation, the formation of cellular infiltrates of stroma in 24 hours, an increase in paresis phenomena, erythrostasis, stroke edema, focal dystrophic changes in cardiomyocytes, small focal cell infiltration after 48 hours, appearance erythro- and plasmapostasis, deepening of edema, fragmentation of fibers and their focal wave-like deformation after 72 hours.
Conclusions. The given data testify to the systemic pathogenic effect of acute lung injury on the organism of experimental animals, in particular on the heart with a maximum of metabolic disturbances in 24 hours and the activation of compensation mechanisms in 48–72 hours.
References
Berezovskyy, V.A. (1978). Gipoksiya i individualnye osobennosti reaktyvnosti [Hypoxia and individual peculiarities of reactivity]. Kyiv: Naukova dumka [in Ukrainian].
Hryshchuk, L.A. & Marushchak, M.I. (2011). Dynamika perekysnoho okysnennia lipidiv ta antyoksydantnoho zakhystu v shchuriv pry hostromu urazhenni lehen [Dynamics of lipid peroxidation and antioxidant defense in rats with acute lung injury]. Tuberkuloz, lehenevi khvoroby ta VIL-infektsiia – Tuberculosis, Pulmonary Diseases and HIV-infection, (1), 16-20 [in Ukrainian].
Levitskyy, A.P., Makarenko, O.A., & Denga, O.V. (2005). Eksperymentalnye metody issledovanyya stymulyatorov osteogeneza. [Experimental methods of study of osteogenesis stimulators] Metodycheskye rekomendatsyy [Methodical recommendations]. Kiev: HFTS MZ Ukrainy “Avitsenna” [in Russian].
Korobeynikova, E.N. (1989). Modifikatsyya opredeleniya produktov POL v reaktsyy s tyobarbyturovoy kyslotoy [Modification of the determination of LPO products in reaction with thiobarbituric acid]. Laboratornoe delo – Laboratory Business, 7, 8-10 [in Russian].
Korolyuk, M.A., Ivanova, L.I., & Mayorova, I.G. (1988). Metod opredeleniya aktivnosti katalazy [Method for determining the activity of catalase]. Laboratornoe delo – Laboratory Business, (1), 16-19 [in Russian].
Marushchak, M.I. (2012). Rol aktyvnykh form kysni u rozvytku i prohresuvanni hostroho urazhennia lehen v eksperymenti [The role of active forms of oxygen in the development and progression of acute lung injury in the experiment]. Medychna ta klinichna khimiia – Medical and Clinical Chemistry, 14 (1), 104-108 [in Ukrainian].
Hudyma, A.A., Marushchak, M.I., Habor, H.H., & Kalitska, M.I. (2011). Patohenna rol neitrofilnykh hranulotsytiv u rozvytku hostroho urazhennia lehen [Pathogenic role of neutrophil granulocytes in the development of acute lung injury]. Bukovynskyi medychnyi visnyk – Bukovyna Medical journal, (3), 82-84 [in Ukrainian].
Sapozhnikov, A.G., & Dorosevych, A.E. (2000). Gistologicheskaya i mikroskopicheskaya tekhnika: rukovodstvo [Histological and Microscopic Technique]. Smolensk: SAU [in Russian].
Chevary, S. (1985). Rol superoksydreduktazy v okyslytelnykh protsessakh kletky i metod opredeleniya ee v biologicheskom materiale [The role of superoxide reductase in cellular oxidative processes and the method of its determination in biological material]. Lab. Delo – Laboratory Business, (11), 678-681 [ in Russian].
Johnson, E.R., & Matthay, M.A. (2010). Acute lung injury: epidemiology, pathogenesis, and treatment. J. Aerosol. Med. Pulm. Drug Deliv. 23 (4), 243-252.
Matute-Bello, G., Frevert, C.W., & Martin, T.R. (2008). Animal models of acute lung injury. American Journal Phisiology, 295 (3), 379-399. doi: 10.1152/ajplung.00010.2008
Liu, K.D., Glidden, D.V., & Eisner, M.D. (2007). Predictive and pathogenetic value of plasma biomarkers for acute kidney injury in patients with acute lung injury. Crit. Care Med., 35, 2755-2761.
Downloads
Published
How to Cite
Issue
Section
License
Authors who sent their manuscript to "Вісник наукових досліджень. Bulletin of Scientific Research" Surgery agree to the following terms:
a. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal. 
b. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
c. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access)
