ASSESSMENT OF THE POSSIBILITY OF SHORT-TERM STORAGE OF PLACENTA EXPLANTS FOR REGENERATIVE MEDICINE
DOI:
https://doi.org/10.11603/2415-8798.2019.2.10271Keywords:
regenerative medicine, explants, placenta, subnormothermia, hypothermia, biotechnologyAbstract
Placental cells, tissues and biologically active substances are promising objects for an effective treatment of pathological conditions of the endocrine, immune and nervous systems. For clinical use of placental derivatives it is necessary to develop methods for their short-term storage and transportation from the clinic to the biotechnological laboratory and in the opposite direction.
The aim of the study – to determine the possibility of subnormothermic (+20°C) and hypothermic (+4°C) storage of placenta explants.
Materials and Methods. Explants were obtained from placentas after a cesarean section. Explants were stored in culture medium under hypothermic and subnormothermic conditions for 96 hours. Explants structure was histologically evaluated every 24 hours, as well as metabolic characteristics of explants according to the tests for the recovery of resazurin, MTT and glucose consumption test.
Results and Discussion. Under subnormothermic conditions placenta explants were found to retain structure, viability and metabolic characteristics for 48 hours; under hypothermic conditions the term of storage is reduced to 24 hours. Typical lesions of placenta explants are reduction of the villi and intervillous spaces, and trophoblast detachment. Recommendations for the short-term storage of placenta explants were developed.
Conclusions. Thus, it was defined that finding (transporting) placenta explants in subnormothermic conditions for up to 48 hours in culture medium ensures the safety of their properties and determines the effectiveness of their clinical use.
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