CHANGES OF LIPID PEROXIDATION AND ANTIOXIDANT DEFENSE OF HIGH AND LOW-RESISTANCE TO ACUTE HYPOXIC HYPOXIA IN RATS OF DIFFERENT SEX IN IMMOBILIZATIONAL STRESS
DOI:
https://doi.org/10.11603/2415-8798.2019.2.10270Keywords:
stress, rats, resistance to hypoxia, lipid peroxidation, antioxidant systemAbstract
Determining the mechanisms of damaging the influence of stress on the heart in persons with different reactivity can contribute to the development of individual methods of correction.
The aim of the study – to determine the effect of immobilization stress on changes in peroxide lipid oxidation and antioxidant defense in rats of different sex with high and low resistance to hypoxia (HR, LR).
Materials and Methods. Stress was modeled 4 times by an hour immobilization of rats on a back down with an interval of 24 hours. In the heart, the diene and triaene conjugates, schiff bases, TBA-active products, superoxide dismutase (SOD), catalase activity in heart and ceruloplasmine and activity of peroxides were determined.
Results and Discussion. In control males with high resistance to hypoxia, in comparison with LR, lower activity of lipid peroxidation, higher activity of SOD, concentration of ceruloplasmine, blood peroxides were observed; in HR-females, in comparison with LR, more diene and triene conjugates, schiff bases, SOD, ceruloplasmine and activity of peroxides; less TBA-active products and catalase activity. Immobilization led to an increase in all investigated products of lipid peroxidation (in all groups of animals the TBA-active products increased the most intensively). The females also significantly increased the schiff bases. This indicates the development of oxidative stress, which is more pronounced in males than in females. In females, lipid peroxidation products metabolize faster, than in males. At the same time, the antioxidant link was activated: the superoxide dismutase activity (more in low resistance to hypoxia animals) and catalase activity (more in high resistance to hypoxia individuals) increased maximally in the females, and in males, the concentration of ceruloplasmin (most notably in low resistance to hypoxia animals). The peroxidase activity of blood decreased in all observed groups of animals.
Conclusions. Immobilization of rats leads to oxidative stress in the heart, but the mechanisms of their development, adaptation and compensation depend on resistance to hypoxia and sex.
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