Correlative interactions between indices of bone metabolism and the implementation of programmed death of blood neutrophils in lipopolysaccharide-induced periodontitis without concomitant pathology and combined with chronic thiolactone hyperhomocysteinemi
DOI:
https://doi.org/10.11603/2311-9624.2021.4.12791Keywords:
periodontitis, hyperhomocysteinemia, bone metabolism, apoptosis, neutrophils, correlationAbstract
Summary. Generalized periodontitis (GP) is a multifactorial disease characterized by a chronic course. The progression of destructive phenomena in periodontal tissues depends on many factors, including concomitant general somatic pathologies, one of which is hyperhomocysteinemia syndrome (HHcy).
The aim of the study – to establish possible correlation interactions between indices of bone metabolism and the number of blood neutrophils with increased generation of reactive oxygen species (ROS), reduced transmembrane mitochondrial potential (ΔΨm) and signs of apoptosis/necrosis in rats with lipopolysaccharide (LPS)-induced periodontitis combined with chronic thiolactone HHcy.
Materials and Methods. The experiments were performed on 48 mature white rats, which were divided into the following groups: I – control group (n=12); II – animals with LPS-induced periodontitis (n=12); III – animals with chronic thiolactone HHcy (n=12); IV – animals with LPS-induced periodontitis combined with HHcy (n=12). Indices of bone metabolism – the activity of alkaline phosphatase and acid phosphatase were determined by a biochemical method. ROS generation, ΔΨm, and signs of apoptosis/necrosis of blood neutrophils were determined on an Epics XL flow cytometer. The relationship between the studied parameters was established based on the results of the correlation analysis using the Pearson correlation coefficient.
Results and Discussion. In rats with LPS-induced periodontitis combined with chronic thiolactone HHcy, a high-strength direct interaction between the activity of acid phosphatase in blood serum and the number of neutrophils with hyperproduction of ROS was established; a direct interaction of moderate strength between the activity of acid phosphatase in the blood serum and the number of neutrophils with reduced ΔΨm; a direct interaction of moderate strength between the activity of acid phosphatase in the blood serum and the number of neutrophils with signs of apoptosis; a direct interaction of moderate strength between the activity of acid phosphatase in the periodontal homogenate and the number of neutrophils with hyperproduction of ROS; a direct high-strength interaction between acid phosphatase activity in the periodontal homogenate and the number of neutrophils with reduced ΔΨm; a direct interaction of moderate strength between the activity of acid phosphatase in the periodontal homogenate and the number of neutrophils with signs of apoptosis.
Conclusions. In rats with LPS-induced periodontitis combined with chronic thiolactone HHcy, a number of significant correlation interactions of high and moderate strength between acid phosphatase activity both in blood serum and in periodontal homogenate and the number of blood neutrophils with hyperproduction of ROS, reduced ΔΨm and signs of apoptosis were revealed, which indicates about the relationship between damage to periodontal tissues and the initiation of programmed death of neutrophils with concomitant HHcy, which requires further study.
References
Butt, K., Butt, R., & Sharma, P. (2019). The burden of periodontal disease. Dental Update, 46(10), 907–913.
Sanz, M., Marco Del Castillo, A., Jepsen, S., Gonzalez-Juanatey, J.R., D'Aiuto, F., Bouchard, P., … Wimmer, G. (2020). Periodontitis and cardiovascular diseases: Consensus report. J. Clin. Periodontol., 47(3), 268-288.
Peres, M.A., Macpherson, L.M.D., Weyant, R.J., Daly, B., Venturelli, R., Mathur, M.R., … Watt, R.G. (2019). Oral diseases: a global public health challenge. Lancet, 394(10194), 249-260.
Mashchenko, I.S., Hudaryan, O.O., & Kucherenko, T.O. (2020). Klinichni, imunolohichni ta metabolichni osoblyvosti zahostrenoho i shvydko prohresuyuchoho variantiv heneralizovanoho parodontyta [Clinical, immunological and metabolic features of acute and rapidly progressive variants of generalized periodontitis]. Sychasna stomatolohiya – Modern Dentistry, 4, 26-32 [in Ukrainian].
AlJehani, Y.A. (2014). Risk factors of periodontal disease: review of the literature. Int. J. Dent, 2014, 182513.
Nechyporuk, V.M., Zaichko, N.V., Melnyk, A.V., Ostrenyuk, R.S., & Korda, M.M. (2019). Vplyv khronichnoyi hiperhomotsysteyinemiyi na metabolizm sirkovmisnykh aminokyslot u nyrkakh shchuriv pry hiper- ta hipotyreozi [The effect of chronic hyperhomocysteinemia on the metabolism of sulfur-containing amino acids in the kidneys of rats in hyper- and hypothyroidism]. Visnyk naukovykh doslidzhen – Bulletin of Scientific Research, 1, 97-102 [in Ukrainian].
Medvedev, D.V., Zvyagina, V.I., & Fomina, M.A. (2014). Sposob modelirovaniya tyazheloy formy gipergomotsisteinemii u krys [Method for modeling severe form of hyperhomocysteinemia in rats]. Ros. mediko-biol. vest. im. akad. I. P. Pavlova – Russian Medical and Biological Bulletin named after Academician I. P. Pavlov, 4, 42-46 [in Russian].
Krivosheyeva, Ye.M., Fefelova, Ye.V., Borodulina, I.I., Sepp, A.V., & Borodulina, N.V. (2010). Effektivnost adaptogenov pri eksperimentalnom parodontite na fone gipergomotsisteinemii [Efficiency of adaptogens in experimental periodontitis on the background of hyperhomocysteinemia]. Byulleten VSNTS SO RAMN – Bulletin VSNTS SB RAMS, 3(73), 221-225 [in Russian].
Penmetsa, G.S., Bhaskar, R.U., & Mopidevi, A. (2020). Analysis of plasma homocysteine levels in patients with chronic periodontitis before and after nonsurgical periodontal therapy using high-performance liquid chromatography. Contemp. Clin. Dent., 11, 266-273.
Botelho, J., Machado, V., Leira, Y., Proença, L., & Mendes, J.J. (2021). Periodontal Inflamed Surface Area Mediates the Link between Homocysteine and Blood Pressure. Biomolecules, 11(6), 875.
Navarro, B.G., Salas, E.J., López, J.L., & Sala, X.P. (2020). Is there a relationship between dental and/or periodontal pathology and values of C-reactive protein, homocysteine and lipoprotein (a) in patients with cardiovascular disease? A Case Control Study. Journal of Current Medical Research and Opinion, 3(05), 451-458.
Stanisic, D., Jovanovic, M., George, A.K., Homme, R.P., Tyagi, N., Singh, M., & Tyagi, S.C. (2021). Gut microbiota and the periodontal disease: role of hyperhomocysteinemia. Can. J. Physiol. Pharmacol., 99(1), 9-17.
Moiseyeva, Ye.G. (2008). Metabolicheskiy gomeostaz i immunnaya reaktivnost organizma v dinamike vospaleniya v tkanyakh parodonta (eksperimentalnoye issledovaniye) [Metabolic homeostasis and immune reactivity of the organism in the dynamics of inflammation in the periodontal tissues (experimental study)]: avtoref. dis. na soiskaniye uchionoi stepeni d-ra med. nauk :14.00.16 [author. dis. for the degree of Dr. med. Science 14.00.16.]. Мoscow [in Russian].
Stangl, G.I. (2007). Homocysteine thiolactone-induced hyperhomocysteinemia does not alter concentrations of cholesterol and SREBP-2 target gene mRNAS in rats. Exp. Biol. Med., 232(1), 81-87.
Levitskiy A.P., Makarenko, O.A., & Denga, O.V. (2005). Eksperimentalnyye metody issledovaniya stimulyatorov osteogeneza. Metodicheskiye rekomendatsii. [Experimental methods for the study of osteogenesis stimulators. Guidelines] – Kyiv: GFC [in Russian].
Levytskyy, A.P., Makarenko, O.A., Khodakov, I.V., & Zelenina, Yu.V. (2006). Fermentatyvnyy metod otsinky stanu kistkovoyi tkanyny [Enzymatic method for assessing the condition of bone tissue]. Odeskyy medychnyy zhurnal – Odessa Medical Journal, 3, 17-21 [in Ukrainian].
Bass, D.A., Parce, J.W., Dechatelet, L.R., Szejda, P., Seeds, M.C., & Thomas, M. (1983). Flow cytometric studies of oxidative product formation by neutrophils: a graded response to membrane stimulation. J. Immunol., 130(4), 1910-1917.
Chechina O.Ye., Ryazantseva N.V., Sazonova Ye.V., Zhukova N.G., Udintseva I.N., & Novitskiy V.V. (2011). Mekhanizmy apoptoza limfotsitov pri kleshchevom entsefalite [Mechanisms of apoptosis of lymphocytes in tick-borne encephalitis]. Byulleten sibirskoy meditsiny – Bulletin of Siberian Medicine, 10(6), 61-65 [in Russian].
Maianski, N.A., Maianski, A.N., Kuijpers, T.W., & Roos, D. (2004). Apoptosis of neutrophils. Acta Haematol., 111(1-2), 56-66.
Becerik, S., Afacan, B., Oztürk, V.Ö., Atmaca, H., & Emingil, G. (2011). Gingival crevicular fluid calprotectin, osteocalcin and cross-linked N-terminal telopeptid levels in health and different periodontal diseases. Dis. Markers., 31(6), 343-352.
Matthews, J.B., Wright, H.J., Roberts, A., Cooper, P.R., & Chapple, I.L. (2007). Hyperactivity and reactivity of peripheral blood neutrophils in chronic periodontitis. Clin. Exp. Immunol.,147(2), 255-264.
Parakhonskiy, A.P., Shmalko, N.M., & Tsyganok, S.S. (2006). Znacheniye apoptoza leykotsitov v immunopatogeneze parodontita [Significance of leukocyte apoptosis in the immunopathogenesis of periodontitis]. Sovremennyye problemy nauki i obrazovaniya – Modern Problems Of Science And Education, 2, 74-75 [in Russian].
Sarkisov, A.K., Polunina, E.A., & Sarkisov, K.A. (2019). Analiz urovnya markera apoptoza anneksina V i stomatologicheskogo statusa u patsiyentov s khronicheskim generalizovannym parodontitom na fone bronkhoektaticheskoy bolezni [Analysis of the level of the apoptosis marker annexin V and dental status in patients with chronic generalized periodontitis against the background of bronchiectasis]. Kubanskiy nauchnyy meditsinskiy vestnik – Kuban Scientific Medical Bulletin, 26(2), 85-92 [in Russian].
Lehotsky, J., Petras, M., Kovalska, M., Tothova, B., Drgova, A., & Kaplan, P. (2015). Mechanisms involved in the ischemic tolerance in brain: effect of the homocysteine. Cell Mol. Neurobiol., 35(1), 7-15.
Huang, R.F., Huang, S.M., Lin, B.S., Wei, J.S., & Liu, T.Z. (2001). Homocysteine thiolactone induces apoptotic DNA damage mediated by increased intracellular hydrogen peroxide and caspase 3 activation in HL-60 cells. Life Sci., 68(25), 2799-2811.
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