Changes of lipids’ peroxide oxidation indices in lipopolysaccharide-induced periodontitis without comorbid pathology and in the background of chronic thiolactone hyperhomocysteinemia
DOI:
https://doi.org/10.11603/2311-9624.2021.3.12463Keywords:
lipopolysaccharide, periodontitis, hyperhomocysteinemia, LPOAbstract
Activation of lipid peroxidation (LPO) plays a significant role in the pathogenesis of generalized periodontitis (GP), causing not only metabolic disturbances, but also structural changes in periodontal tissues. According to various sources, in 85–97% of cases, GP is combined with somatic pathology, which makes a significant difference in the etiopathogenesis of periodontal diseases. One of these pathologies that can complicate the course of GP is hyperhomocysteinemia syndrome (HHcy).
The aim of the study – to establish the features of LPO in the blood serum and periodontal homogenate of rats with lipopolysaccharide (LPS) -induced periodontitis combined with chronic thiolactone HHcy.
Materials and Methods. The experiments were performed on 48 mature white rats, which were divided into the following groups: I – control group (n=12); II – animals with LPS-induced periodontitis (n=12); III – animals with chronic thiolactone HHcy (n = 12); IV – animals with LPS-induced periodontitis combined with HHcy (n = 12). The intensity of LPO was determined by the content of lipid hydroperoxides (HPL) and active products of thiobarbituric acid (TBA-AP).
Results and Discussion. It was found that the content of HPL in the blood serum of rats with LPS-induced periodontitis increased by 52.8 % (p=0.004), and in rats with LPS-induced periodontitis combined with chronic thiolactone HHcy – by 2.2 times (p<0.001) vs. control group. In the periodontal homogenate, the content of HPL in animals with LPS-induced periodontitis increased by 2.0 times (p=0.002), and in rats with combined pathology – by 3.1 times (p<0.001) vs. control group. The intensity of changes in TBA-AP was higher vs. HPL. So, in rats with LPS-induced periodontitis combined with chronic thiolactone HHcy, this index significantly increased by 3.0 times (blood serum) and by 4.4 times (periodontal homogenate) vs. control group, which significantly exceeded the data for LPS-induced periodontitis without comorbid pathology ( by 54.5 % and 51. 1 %, respectively).
Conclusions. LPS-induced periodontitis in rats is accompanied by a significant increase in the intensity of LPO processes both in the blood serum and in the periodontal homogenate. Chronic thiolactone HHcy enhances oxidative stress in periodontitis, as evidenced by significantly higher TBA-AP values in the studied biological fluids of rats with combined pathology compared to animals with LPS-induced periodontitis without comorbid pathology.
References
Nocini, R., Lippi, G., & Mattiuzzi, C. (2020). Periodontal disease: the portrait of an epidemic. JPHE, 4, 10.
Kolenko, Yu.G., Volovyk, I.A., & Myalkivsky, K.O. (2021). Vplyv zakhvoryuvan tkanyn parodonta na yakist zhyttya patsiyentiv [The influence of periodont tissue diseases on the quality of life of patients]. Sychasna stomatolohiya – Modern Dentistry, 2, 36-42 [ in Ukrainian].
Saveleva, N.M., Sokolova, I.I., German, S.I., & Tomilina, T.V. (2018). Imunolohichni aspekty heneralizovanoho parodontytu (ohlyad literatury) [Immunological aspects of generalized periodontitis (literature review)]. Visnyk naukovykh doslidzhen – Bulletin of Scientific Research, 2, 110-115.
Peres, M.A., Macpherson, L.M.D., Weyant, R.J., Daly, B., Venturelli, R., Mathur, M.R., Listl, S., Celeste, R.K., Guarnizo-Herreño, C.C., Kearns, C., Benzian, H., Allison, P., & Watt, R.G. (2019). Oral diseases: a global public health challenge. Lancet, 394(10194), 249-260.
Shcherba, V.V., & Korda, M.M. (2018). Funktsionalnyy stan systemy antyoksydnoho zakhystu u shchuriv z parodontytom na foni hiper- ta hipotyreozu [Functional state of the antioxidant defense system in rats with periodontitis on the background of hyper- and hypothyroidism.]. Bukovynskyy medychnyy visnyk – Bukovynian Medical Bulletin, 22, 2 (86), 129-137 [in Ukrainian].
Savelyeva, N.N. (2015). Sostoyaniye sistemy perekisnogo okisleniya lipidov i antioksidantnoy zashchity u bolnykh khronicheskim generalizovannym parodontitom I-II stepeni tyazhesti, sochetayushchegosya s parazitozami [The state of the system of lipid peroxidation and antioxidant protection in patients with chronic generalized periodontitis I-II severity, combined with parasitosis]. Journal of Education, Health and Sport, 5(12), 465-476 [ in Russian].
Krynytska, I, Marushchak, M, Svan, O, Akimova, V, Mazur, L, & Habor, H. (2018). The indices of endogenous intoxication in rats with carrageenan solution consumption. Georgian Med. News, 279, 196-200.
Gulenko, O.V., Faraponova, Y.E.A., Volobuyev, V.V., & Bykova, N.I. (2014). Sostoyaniye perekisnogo okisleniya lipidov pri zabolevaniyakh parodonta u detey s psikhonevrologicheskimi narusheniyami [The state of lipid peroxidation in periodontal diseases in children with neuropsychiatric disorders]. Mezhdunarodnyy zhurnal prikladnykh i fundamental'nykh issledovaniy – International Journal of Applied and Basic Research, 2, 59-64 [in Russian].
Pupin, T.I., Moroz, K.A., Vynohradova, O.M., Hnid, R.M., Hnid, M.R., & Sahaydak, T.V. (2021). Heneralizovanyy parodontyt i podahra: porivnyannya patohenetychnykh mekhanizmiv rozvytku (ohlyad literatury) [Generalized periodontitis and gout: a comparison of pathogenetic mechanisms of development (literature review).]. Klinichna stomatolohiya – Clinical Dentistry, 1, 44-53 [ in Ukrainian].
Nemesh, O.M., Honta, Z M., Slaba, O.M., & Shylivskyi, I.V. (2021). Pathogenetic mechanisms of comorbidity of systemic diseases and periodontal pathology. Wiad. Lek, 74(5), 1262-1267.
Krivosheyeva, Ye.M., Fefelova, Ye.V., Borodulina, I.I., Sepp, A.V., & Borodulina, N.V. (2010). Effektivnost adaptogenov pri eksperimentalnom parodontite na fone gipergomotsisteinemii [Efficiency of adaptogens in experimental periodontitis on the background of hyperhomocysteinemia]. Byulleten VSNTS SO RAMN – Bulletin VSNTS SB RAMS, 3(73), 221-225 [ in Russian].
Yurchenko, P.O., Melnyk, A.V., Zaichko, N.V., & Yoltukhivskyy, M.M. (2014). Osoblyvosti obminu homotsysteyinu ta hidrohen sulfidu v tsentralniy nervoviy systemi [Features of homocysteine and hydrogen sulfide metabolism in the central nervous system]. Medychna khimiya –Medical Chemistry, 3(60), 90-96 [ in Ukrainian].
Nechyporuk, V.M., Zaichko, N.V., Melnyk, A.V., Ostrenyuk, R.S., & Korda, M.M. (2019). Vplyv khronichnoyi hiperhomotsysteyinemiyi na metabolizm sirkovmisnykh aminokyslot u nyrkakh shchuriv pry hiper- ta hipotyreozi [The effect of chronic hyperhomocysteinemia on the metabolism of sulfur-containing amino acids in the kidneys of rats in hyper- and hypothyroidism]. Visnyk naukovykh doslidzhen – Bulletin of Scientific Research, 1, 97-102 [ in Ukrainian].
Nekrut, D.O. (2016). Vplyv hiperhomotsysteinemii na formuvannia nealkoholnoi zhyrovoi khvoroby pechinky u shchuriv [The effect of hyperhomotsisteinemia on the formation of nonalcoholic fatty liver disease in rats]. Visnyk morfolohii – Bulletin of Morphology, 1(22), 40-45 [in Ukrainian].
Smith, A.D., & Refsum, H. (2021) Homocysteine – from disease biomarker to disease prevention. J. Intern. Med., doi: 10.1111/joim.13279. Epub ahead of print. PMID: 33660358.
Rehman, T., Shabbir, M.A., Inam-Ur-Raheem, M., Manzoor, M.F., Ahmad, N., Liu Z.W., Ahmad, M.H., Siddeeg, A., Abid, M., & Aadil, R.M. (2020). Cysteine and homocysteine as biomarker of various diseases. Food Sci. Nutr., 8, 4696-4707.
Cordaro, M., Siracusa, R., Fusco, R., Cuzzocrea, S., Di Paola, R., & Impellizzeri, D. (2021). Involvements of hyperhomocysteinemia in neurological disorders. Metabolites, 11, 37.
Salvio, G., Ciarloni, A., Cutini, M., & Balercia, G. (2021). Hyperhomocysteinemia: Focus on endothelial damage as a cause of erectile dysfunction. Int. J. Mol. Sci., 22, 418.
Mallapragada, S., Kasana, J., & Agrawal, P. (2017). Effect of Nonsurgical Periodontal Therapy on Serum Highly Sensitive Capsule Reactive Protein and Homocysteine Levels in Chronic Periodontitis: A Pilot Study. Contemp. Clin. Dent., 8(2), 279-285.
Penmetsa, G.S., Bhaskar, R.U., & Mopidevi, A. (2020). Analysis of plasma homocysteine levels in patients with chronic periodontitis before and after nonsurgical periodontal therapy using high-performance liquid chromatography. Contemp. Clin. Dent., 11, 266-273.
Botelho, J., Machado, V., Leira, Y., Proença, L., & Mendes J.J. (2021). Periodontal Inflamed Surface Area Mediates the Link between Homocysteine and Blood Pressure. Biomolecules, 11(6), 875.
Navarro, B.G., Salas, E.J., López, J.L., & Sala, X.P. (2020). Is there a relationship between dental and/or periodontal pathology and values of C-reactive protein, homocysteine and lipoprotein (a) in patients with cardiovascular disease? A Case Control Study. Journal of Current Medical Research and Opinion, 3(05), 451-458.
Stanisic, D., Jovanovic, M., George, A.K., Homme, R.P., Tyagi, N., Singh, M., & Tyagi, S.C. (2021). Gut microbiota and the periodontal disease: role of hyperhomocysteinemia. Can. J. Physiol. Pharmacol., 99(1), 9-17.
Moiseyeva, Ye.G. (2008). Metabolicheskiy gomeostaz i immunnaya reaktivnost' organizma v dinamike vospaleniya v tkanyakh parodonta (eksperimental'noye issledovaniye) [Metabolic homeostasis and immune reactivity of the organism in the dynamics of inflammation in the periodontal tissues (experimental study)]: avtoref. dis. na soiskaniye uchionoi stepeni d-ra med. nauk :14.00.16 [author. dis. for the degree of Dr. med. Science 14.00.16. ]. М. [ in Russian ].
Stangl, G.I. (2007). Homocysteine thiolactone-induced hyperhomocysteinemia does not alter concentrations of cholesterol and SREBP-2 target gene mRNAS in rats. Exp. Biol. Med., 232(1), 81-87.
Vlizlo, V.V., Fedoruk, R.S., & Ratych I.B. (2018). Laboratorni metody doslidzhen u biolohii, tvarynnytstvi ta veterynarniy medytsyni: dovidnyk [Laboratory research methods in biology, animal husbandry and veterinary medicine: a handbook]. Lviv : SPOLOM. [in Ukrainian].
Lowry, O.H., Rosenbrough, N.G., Farr A.L., & Randall, R.C. (1951). Protein measurement with the Folin phenol reagent. J. Biol. Chem., 193(1), 265-275.
Chudinova, T.N. (2015). Obosnovaniye i taktika primeneniya sredstv metabolicheskoy korrektsii v kompleksnom lechenii vospalitelnykh zabolevaniy parodonta u bolnykh s polimorbidnoy patologiyey vnutrennikh organov [Justification and tactics of the use of metabolic correction means in the complex treatment of inflammatory periodontal diseases in patients with polymorbid pathology of internal organs]: avtoref. dis. na soiskaniye uchionoi stepeni d-ra med. nauk :14.00.14 [author. dis. for the degree of Dr. med. Science 14.00.14. ]. Sankt Peterburg [ in Russian].
Balitska, O.Yu., Bondarenko, Yu.I., & Habor, H.H. (2018). Aktyvnist protsesiv peroksydatsiyi lipidiv u khvorykh na khronichnyy heneralizovanyy parodontyt i tsukrovyy diabet 2 typu [Activity of lipid peroxidation processes in patients with chronic generalized periodontitis and type 2 diabetes mellitus].Visnyk naukovykh doslidzhen – Bulletin of Scientific Research, 3, 98-101 [ in Ukrainian ].
Shcherba, V.V., Krynytska, I.Ya., Cherkashyn, S.I., Machohan, V.R. , Stoykevych, H.V., & Korda, M.M. (2018). Stan peroksydnoho okysnennya lipidiv u shchuriv z parodontytom na foni hiper- ta hipotyreozu [The state of lipid peroxidation in rats with periodontitis on the background of hyper- and hypothyroidism]. Svit medytsyny ta biolohiyi – The World of Medicine and Biology, 2 (64), 185-189 [ in Ukrainian].
Cheremisina, V.F., Zhemela, O.D. & Huchenko, H.P. (2018). Stan vilnoradykalnykh protsesiv ta systemy antyoksydantnoho zakhystu spoluchnoyi tkanyny parodonta u kroliv pry hidrokortyzonovomu parodontyti [The state of free radical processes and the system of antioxidant protection of periodontal connective tissue in rabbits with hydrocortisone periodontitis]. VISNYK Ukrayinska medychna stomatolohichna akademiya – Bulletin of Ukrainian Medical Dental Academy, 18, 3 (63), 190-193 [ in Ukrainian].
Lubos, E., Loscalzo, J., & Handy, D.E. (2007). Homocysteine and glutathione peroxidase-1. Antioxid. Redox. Signal., 9(11), 1923-1940.
Dayal, S., Baumbach, G.L., Arning, E., Bottiglieri, T., Faraci, F.M., & Lentz, S.R. (2017). Deficiency of super- oxide dismutase promotes cerebral vascular hypertrophy and vascular dysfunction in hyperhomocysteinemia. PLoS One, 12(4), e0175732.
Wu, Y, Yang, L, & Zhong, L. (2010). Decreased serum levels of thioredoxin in patients with coronary artery disease plus hyperhomocysteinemia is strongly associated with the disease severity. Atherosclerosis, 212(1), 351-355.
Yuyun, M.F., Ng, L.L., & Ng, G.A. (2018). Endothelial dysfunction, endothelial nitric oxide bioavailability, tetrahydrobiopterin, and 5-methyltetrahydrofolate in cardiovascular disease. Where are we with therapy? Microvasc. Res., 119, 7-12.
Nadesalingam, A., Chen, J.H.K., Farahvash, A., & Khan, M.A. (2018). Hypertonic Saline Suppresses NADPH Oxidase-Dependent Neutrophil Extracellular Trap Formation and Promotes Apoptosis. Front. Immunol., 9, 359.
Zvyagina, V.I., Belskikh, E.S., Uryasyev, O.M., Medvedev, D.V., Kiseleva, V.A., & Tverdova, L.V. (2018). Vliyaniye karnitina khlorida na mitokhondrii serdtsa krys pri modelirovanii gipergomotsisteinemii [Effect of carnitine chloride on the mitochondria of the rat heart in the simulation of hyperhomocysteinemia.]. Meditsinskiy vestnik Severnogo Kavkaza – Medical Bulletin of the North Caucasus, 13 (1.1), 78-81 [in Russian].
Myslivets, M.G., Naumov, A.V., & Paramonova, N.S. (2017). Rol gomotsisteina v razvitii yuvenilnogo revmatoidnogo artrita [The role of homocysteine in the development of juvenile rheumatoid arthritis.]. Zhurnal Grodnenskogo gosudarstvennogo meditsinskogo universiteta – Journal of Grodno State Medical University, 2, 144-148 [ in Russian].
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