Dynamics of biochemical and microbiological parameters in modelling different reactivity of the organism
DOI:
https://doi.org/10.11603/2311-9624.2020.1.12042Keywords:
biochemical parametersAbstract
Summary. Periodontal diseases in socio-economic importance are among the most difficult problems of modern dentistry. The issues of clarifying the key mechanisms of development and nature of inflammatory and destructive lesions of periodontal tissues depending on the condition of reactivity of the organism and the ability to control these processes remain relevant.
The aim of the research was to study the dynamics of biochemical and microbiological parameters in the experiment with modelling the extreme conditionss of hyper- and hyporeactivity of the organism.
Materials and methods. The experiments were performed on 24 adult not purebred dogs weighing 8-12 kg with spontanious periodontitis. Animals were divided into three levels of groups, where the states of normo-, hyper- and hyporeactivity of the organism were stimulated. In the blood serum and gum tissues the dynamics of biochemical parameters was assessed, as well as the frequency of secretion and the level of microflora population of the dental biofilm in cases of different reactivity of the organism.
Research results and their discussion. When the body is hyper- and hyporeactive, the reserve capacity of the antioxidant system (AOC) is depleted, as evidenced by a significant decrease in the activity of the main enzyme of the anti-radical link of AOC - superoxide dismutase (SOD) - in serum and gum biopsy, respectively, 1.41 and 2.84 times more (p <0.05). It causes a significant increase in the activity of the enzyme phospholipase A2 in serum and gum tissue (p <0.05). The analysis of the frequency of secretion and the level of microflora population of dental biofilm in animals with spontaneous periodontitis against impaired reactivity of the organism indicates the predominance of aerobic-anaerobic associations with increasing detection of facultative and obligate anaerobes.
Conclusions. Modelling of impaired (hyper- and hypo-) reactivity of the organism provokes the imbalance of regulatory systems, which is manifested by the apparent destruction of periodontal tissues and the corresponding dynamics of biochemical and microbiological parameters. Therefore, we consider further studying of the possibility of influencing the reactivity of the organism with their approximation to the values of the body᾽s normoreaction promosing, which will provide the optimal development of the pathological process in the periodontium.
References
Borysenko, А.V., & Mialkivkyi, C.О. (2015). Obgruntuvannia vyboru zasobіv іndyvіdualnoi hіhіieny porozhnyny rota pry lіkuvannі urazhen marhіnalnoho parodontu [Rationale for the choice of personal hygiene in the treatment of marginal periodontal lesions]. Sovremennaya stomatologiya – Modern Dentistry, 2, 24–27 [in Ukrainian].
Zabolotnyi, Т.D., Pupin, Т.І., & Borisenko, А.V. (2013). Zapalnі zahvoriuvannia parodonta [Inflammatory diseases of the periodontium]. Lvіv: GalDent [in Ukrainian].
Petrushanko, Т.О. (2001). Іntehralnyi іndyvіdualnyi pіdhіd u profіlaktytsi zakhvoriuvan parodonta [An integrated individual approach in the prevention of periodontal disease]. Doctor᾽s thesis. Kiev [in Ukrainian].
Alkan, A., Cakmak, O., Yilmaz, S., Cebi, T., & Gurgan C. (2015). Relationship between psychological factors and oral health status and behaviours. Oral Health Prev. Dent, 13 (4), 331-339. DOI: 10.3290/j.ohpd.a32679
Jenkins, W.M., & Papapanou, P.N. (2000). Epidemiology of periodontal diseases in children and adolescents. Periodontology, 26, 16-32.
Slots, J. (2017). Periodontitis: facts, fallacies and the future. Periodontology, 1 (75), 7–23. DOI: 10.1111/prd.12221
Grinin, V.М., Vagner, V.D., Cabak, D.S., Epifanov, S.A., & Jivotov, V.A. (2019). Sostoyanie tkanei parodonta u patsiyentov, nakhodyaschihsya na lechenii v mnogoprofilnom statsionare [The condition of periodontaltissues for patients undergoing treatment in a multidisciplinary hospital]. Institut stomatologii – Institute of Dentistry, 3, 31–34 [in Russian].
Cononova, О.V. (2019). Vіddalenі rezultaty lіkuvannia khvorykh na heneralіzovanyi parodontyt z proiavamy psikhoemotsіinoho stresu [Long-term results of treatment of patients with generalized periodontitis with manifestations of psychoemotional stress]. Novyny stomatolohii – Dentistry News, 2 (99), 6-10 [in Ukrainian].
Petrushanko, Т.О., Vhereda, G.A., & Loban, V.V. (2017). Stres-іndukovanі zmіny mіkrobіomu yasennoi borozny vіd gendernoho faktoru [Stress-induced changes in the microbiome of the gingival sulcus from the gender factor]. Vіsnyk problem bіolohii і medytsyny – Bulletin of Problems of Biology and Medicine, 1 (139), 240–242 [in Ukrainian].
Pashaev, А.Ch., Gasanov, V.M., & Guseinova, S.T. (2020). Analiz faktorov riska vospalitelnyh zabolevaniy parodonta [Analysis of risk factors for inflammatory periodontal diseases]. Sovremennaya stomatologіya – Modern Dentistry, 1, 39–41 [in Russian].
Samoilenko, А.V. (2003). Suchasnі aspekty etіolohii, patohenezu ta lіkuvannia rіznykh klіnіchnykh varіantіv heneralіzovannoho parodontytu [Modern aspects of etiology, pathogenesis and treatment of different clinical variants of generalized periodontitis] Doctor᾽s thesis. Odessa [in Ukrainian].
Silenko, Y.І. (2000). Klіnіko-patohenetychne obgruntuvannia lіkuvannia heneralіzovanoho parodontytu z vykorystanniam nyzkomolekulyarnykh polіpeptydnykh preparatіv [Clinical and pathogenetic substantiation of treatment of generalized periodontitis using low molecular weight polypeptide drugs] Doctor᾽s thesis. Poltava [in Ukrainian].
Мelnichuk, G.М. (2013). Tsytokinovyi profil sliuny u bolnyh generalizovanym parodontitom [Cytokine profile of saliva in patients with generalized periodontitis]. Sovremennaya stomatologiya – Modern Dentistry, 1, 35-40 [in Russian].
Repetska, О.М., Rozhko, M.M., Skrypnyk, N.V., & Ilnytska, O.M. (2020). Poshyrenіst ta іntensyvnіst zakhvoriuvan tkanyn parodonta v osіb molodoho vіku na tlі pervynnoho hіpotyreozu [Prevalence and intensity of periodontal diseases in young people on the background of primary hypothyroidism]. Suchasna stomatologіya – Modern Dentistry, 1, 42–48 [in Ukrainian].
Markovska, І.V., & Sokolova, І.І. (2019). Dinamіka stomatolohіchnoho statusu patsіientіv, yakі pіddaiutsia vplyvu neіonіzuiuchoho nyzkochastotnoho elektromahnіtnoho vypromіniuvannia promyslovoi chastoty (70 kHts) [Dynamics of the dental status of patients exposed to non-ionizing low-frequency electromagnetic radiation of industrial frequency (70 kHz)]. East Scientific Journal, 9 (2), 16–19 [in Ukranian].
Copchak, О.V., Biloklicska, G.F., Stechenko, L.О., & Krivosheeva, О.І. (2017). Ultrastrukturna orhanіzatsіia tkanyn yasen khvorykh na heneralіzovanyi parodontyt pry kardіovaskuliarnіi patolohіi [Ultrastructural organization of gum tissue in patients with generalized periodontitis with cardiovascular pathology]. Svіt medytsyny ta bіolohii – World of Medicine and Biology,
(59), 121–126 [in Ukranian].
Sokrut V. N. (1992). Formy reaktivnosti organizma i zazhivlenie infarkta miokarda [Forms of body reactivity and healing of myocardial infarction] Doctor᾽s thesis. Donetsk [in Russian].
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2021 CLINICAL DENTISTRY
This work is licensed under a Creative Commons Attribution 4.0 International License.
