Clinical-genetic and inter-genetic correlation analysis with expression of miR-29а in patients with pleomorphic adenomas of the salivary glands
DOI:
https://doi.org/10.11603/2311-9624.2020.4.11714Keywords:
плеоморфні аденоми, генетичні дослідження, miR-29a, апопотоз, клініко-генетичні кореляції, міжгенетичні кореляціїAbstract
SUMMARY. The most common tumor remains pleomorphic adenoma – 60–90 % of all benign tumors of the salivary glands. The modern genetic area of focus in the diagnosis of salivary gland tumors is the study of the role of miRNA molecules. MiRNAs are small non-coding RNAs that regulate the cell cycle, apoptosis, metabolism, cell development and differentiation. Of the greatest interest among them is proapoptotic miR-29a. It is expressed in 84 % of the pleomorphic adenomas of the salivary glands. There are no articles that would describe the clinical-genetic and inter-genetic correlations with miR-29a in other biopsy specimens, except for the tumor – the salivary gland tissue adjacent to the tumor, the intact gland (control group), and blood.
The aim of the study – clinical-genetic and inter-genetic (with expression of miR-29а) correlation analysis in tissue of pleomorphic adenomas of the large salivary glands that adjacent to the tumor of the tissue of the salivary gland, intact tissue of the salivary gland, was out of touch with the tumor and venous blood.
Materials and Methods. 22 patients with benign tumors of the large salivary glands (pleomorphic adenomas) were used examination materials. The expression of miR-29a was evaluated using reverse transcription and quantitative polymerase chain reaction (qPCR) in real time.
Results and Discussion. Conducted analysis of the level of expression of miRNA–29a revealed that among 4 groups of indicators (tumor, tissue adjacent to the tumor salivary gland tissue, intact salivary gland that was out of touch with the tumor and venous blood) in patients with pleomorphic adenoma of the large salivary gland the highest expression was noted in the group – salivary gland tissue adjacent to the tumor – (111.93±56.97).
Conclusions. Conducted correlation analysis of clinical (age, size of the tumor, duration of the disease, distance from the surface of the skin to the tumor) and genetic indices for the level of expression miR-29a in different biological tissues (tumor, tissue adjacent to the tumor salivary gland tissue, intact salivary gland that was out of touch with the tumor and venous blood) in patients with pleomorphic adenoma of the large salivary glands revealed presence one statistically significantly association (miR-29a). Inter-genetic correlation analysis of patients with PA of the large salivary glands (with level of expression miR-29a) with different fragments of biopsy material revealed absence of another statistically significantly association.
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