Dynamics of phosphatase activity in blood serum and periodontal tissue homogenate in experimental gingivitis
DOI:
https://doi.org/10.11603/2311-9624.2020.3.11573Keywords:
experimental gingivitis, blood serum, periodontal tissue homogenate, phosphatase activityAbstract
Summary. Due to the significant spread of inflammatory diseases of periodontal tissues, improving the results of their treatment and prevention remains an urgent issue in dentistry. To reveal the links of pathogenesis that have not been studied yet and to conduct the most effective treatment, it is optimal to study the course of biochemical processes in experimental gingivitis and its correction.
The aim of the study – evaluation of phosphatase activity in blood serum and gum homogenate in experimental gingivitis and its correction in adult rats.
Materials and Methods. The experiments were performed on 100 white nonlinear male rats aged 5–6 months: 10 intact rats; 30 rats with experimental gingivitis without changes in the reactivity of the body, 30 rats with experimental gingivitis on the background of hypoergia of the body, 30 rats with experimental gingivitis on the background of hyperergy of the body, which was treated with anti-inflammatory gel with Neovitin. Slaughter of animals and collection of blood and soft tissues of periodontium under thiopental anesthesia was performed 7 days after the start of the experiment in the group with simulated gingivitis, in groups without and with correction – after 14 days.
Results and Discussion. It was found that changes in alkaline phosphatase activity in the homogenate with the correction of gingivitis were as follows: an increase in alkaline phosphatase activity was observed in normo- and hypoergic rats 1.2 times (p <0.05) and 5.2 % (p> 0.05), accordingly, in the hyperergic group there was a decrease in alkaline phosphatase activity by 1.45 times (p <0.05) compared with the corresponding indicators of rats without correction. Correction with anti-inflammatory gel with Neovitin reduced the activity of sour phosphatase in the homogenate of the gums in all groups of animals: 1.5, 1.2 and 1.7 times in the normo-, hypo- and hyperergic groups, respectively, when compared with the corresponding group of animals without treatment.
Conclusions. Changes in phosphatase activity indicated an adaptive response of the body and gums, in particular, to the development of the inflammatory process in the periodontium. The use of anti-inflammatory gel with Neovitin in white rats in experimental gingivitis had a more pronounced corrective effect on the whole body of experimental animals.
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