Determination of values of stress-factors in patients of Lviv Regional Clinical Hospital with dystrophic and inflammatory diseases of periodontal tissues in the blood and oral cavity
DOI:
https://doi.org/10.11603/2311-9624.2020.3.11568Keywords:
dystrophic-inflammatory diseases of periodontal tissues, ɑ-amylase, glucose, cortisol, somatic diseasesAbstract
Summary. In order to study stressors in the blood and oral fluid of persons with dystrophic and inflammatory diseases of periodontal tissues, who were treated in different departments of Lviv Regional Clinical Hospital (LRCH), the activity of ɑ-amylase, glucose and cortisol was determined.
The aim of the study – determination of values of stress factors in patients of LRCН with dystrophic-inflammatory diseases of periodontal tissues.
Materials and Methods. ɑ-amylase activity and glucose concentration in human blood and oral fluid were determined by enzymatic colometric method. The calculation of the concentration of cortisol in the blood and oral fluid of patients was performed using solid-phase enzyme-linked immunosorbent assay on a device StatFax 303 using a test kit "Steroid ELISA-cortisol" company Alcor-Bio. Blood sampling in patients was performed in the morning, on an empty stomach, after a 12-hour break in food, from the ulnar vein, oral fluid for research was collected in the morning on an empty stomach, by spitting into measuring tubes, without stimulation. To assess the probability of the obtained results of the study used a variational-statistical method of analysis using Microsoft Excel. Statistical calculation of the results of clinical and laboratory studies was carried out according to conventional methods.
Results and Discussion. On average, ɑ-amylase activity in patients with dystrophic-inflammatory diseases of periodontal tissues was higher compared to control: in the blood – 1.5 times, p <0.01 and in oral fluid – 2.0 times, p <0.05. Glucose content on average, in patients with dystrophic-inflammatory diseases of periodontal tissues in various somatic diseases was higher than the corresponding data in the control: in the blood – 1.3 times, p <0.05 and in the oral fluid – 1.8 times, p <0.01. The content of cortisol on average in patients with dystrophic-inflammatory diseases of periodontal tissues was higher than in the control group: in the blood – 1.5 times and in the oral fluid – 1.8 times, p <0.05.
Conclusions. Thus, increased ɑ-amylase activity, glucose and cortisol concentrations in patients with periodontal diseases in the blood and oral fluid may indicate a decrease in stress resistance and be objective markers of stress on the body due to the aggravating effects of somatic and periodontal diseases.
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