Modifications of the cytokine profile in blood of rats in periodontitis
DOI:
https://doi.org/10.11603/2311-9624.2019.4.10884Keywords:
cytokines, periodontitis, lipopolysacharideAbstract
Diseases of periodontal tissues occupy one of the first places in frequency and prevalence among dental diseases, also represent a complex problem that is becoming of significant nature. The high incidence of periodontal disease, the severity of some forms of periodontal pathology, loss of teeth and as a result significant changes in the dentition system, reduced performance, reduced quality of life – all this allows us to consider periodontal disease not only a serious medical but also an important social problem.
The aim of the study – to learn changes in the cytokine profile in lipopolysaccharide inflammation of periodontal tissues in experiment.
Materials and Methods. The experiment included 62 experimental animals, which were divided into two groups: I – control animals; II – animals with periodontitis model. To simulate acute periodontitis in experimental animals, we used lipopolysaride, animals of this group were injected with 40 microliters (1mg/ml) lipopolysaccharide (LPS) gums and intragastric 1 % starch solution within 2 weeks. Euthanasia of rats was performed by bloodletting under conditions of thiopental sodium anesthesia on the 22nd day from the beginning of the experiment.
Results and Discussion. The results of the study showed that TNF-α - increased in the serum of animals 8.7 times compared with the control group. At the same time, IL-1β increased 4.2-fold compared with control
(p <0.05). The results of the level of anti-inflammatory cytokines in lipopolysaccharide periodontitis significantly decreased. The data of our studies showed that the level of IL-4 in the serum of experimental animals decreased 1.6 times (p <0.05) relative to control. We also observed a decrease in the serum of rats (1.5-fold compared with the control group) of IL-10.
Conclusions. The results of the studies showed that the development of periodontitis is accompanied by significant changes in the state of immune mechanisms of protection of the oral cavity. The results of the experimental study indicate that with generalized lipopolysaccharide periodontitis in the serum of experimental animals there is an imbalance between the production of proinflammatory and anti-inflammatory cytokines.
References
Borysenko, A.V. (2004). Aktualni problemy etiolohii, patohenezu ta klasyfikatsii zakhvoriuvan parodonta [Actual problems of etiology, pathogenesis and classification of periodontal diseases]. Naukovyi visnyk natsionalnoho medychnoho universyteta imeni
O.O. Bohomoltsia – Scientific Bulletin of the National Medical University by O.O. Bohomolets, 1-2, 55-61 [in Ukrainian].
Duda, K.M., Klishch, I.M., Marushchak, M.I., & Vons, B.V. (2003). Riven prozapalnykh tsytokiniv u syrovattsi krovi shchuriv za umov hostroho parodontytu na tli tsukrovoho diabetu 2 typu [The level of proinflammatory cytokines in the serum of rats under conditions of acute periodontitis on the background of type 2 diabetes]. Visnyk problem biolohii i medytsyny – Bulletin of Problems of Biology and Medicine, 4 (1), 318-321 [in Ukrainian].
Volf, G.F., & Rateytskhak, E.M. (2008). Parodontologiya [Periodontology]. Moscow: MEDpress-inform [in Russian].
Derkach, L.Z., Piuruk, V.P., Pantus, A.V., & Hopko, M.I. (2009). Zminy klitynoho skladu yasenoi ridynu u khvorykh na heneralizovanyi parodontyt ta metody korektsii u kompleksnomu likuvanni zahvoriuvan parodonta [Changes in the cellular composition of gum fluid in patients with generalized periodontitis and methods of their correction in the complex treatment of periodontal diseases]. Halytskyi likarskyi visnyk – Galician Medicinal Herald, 16 (4), 42-45 [in Ukrainian].
Zhou, Q., Desta, T., & Fenton, M. (2005). Cytocine profiling of macrophages exosed to porphyromonas gingivales, its ipopolysaccharide, or its fimA protein. Infection and Immunity, 73 (2), 935-943. DOI: https://doi.org/10.1128/IAI.73.2.935-943.2005
Ezzo, P.J. (2003). Microorganisms as risk indicators for periodontal disease. Periodontol., 32, 24-35. DOI: https://doi.org/10.1046/j.0906-6713.2003.03203.x
Denha, O.V., Tsevukh, D.B., & Levitskyy, A.P. (2007). Biokhimicheskiye pokazateli tkaney parodonta pri eksperimentalnoy terapii periodonta [Biochemical parameters of periodontal tissues during experimental periodontal therapy]. Vísnik stomatologíi – Bulletin of Dentistry, 4, 40-44 [in Ukrainian].
Chumakova, Yu.H. (2008). Rol mistsevykh chynnykiv porozhnyny rota u rozvytku parodontytu [The role of local factors of the oral cavity in the development of periodontitis]. Implantolohiia, parodontolohiia, osteolohiia – Implantology, Periodontics, Osteology, 3, 70-75 [in Ukrainian].
Beliaieva, O.V., & Kevorkov, N.N. (2002). Vliyaniye kompleksnoy terapii na pokazateli mestnogo immuniteta bolnykh parodontitom [The effect of complex therapy on indicators of local immunity of patients with periodontitis]. Tsitokiny i vospaleniye – Cytokines and Inflammation, 1 (4), 34-37 [in Russian].
Lee, J.Y., Yi, N.N., & Kim, U.S. (2006). Porphyromonas gingivalis heat shok protein vaccine reduces the alveolar bone loss induced by multiple periodontopathogenic bacteria. Periodontal. Res., 41 (1), 10-14. DOI: https://doi.org/10.1111/j.1600-0765.2005.00832.x
