Microbiological profile quantitative changes evaluation among patients with stage I periodontitis

  • M. Y. Goncharuk-Khomyn Uzhhorod National University
  • N. I. Melnychuk Uzhhorod National University
  • S. S. Tsuperyak Uzhhorod National University
  • Kh. V. Pohoretska I. Horbachevsky Ternopil National Medical University
  • L. O. Patskan I. Horbachevsky Ternopil National Medical University
Keywords: periodontitis, periodontal pathogens, laboratory analysis

Abstract

Резюме. Periodontitis occurence evidenced by the influence of specific types of microorganisms categorized into appropriate complexes, but the nature of the pathology and the effectiveness of treatment are determined by the complex interaction between pathogenetic factors and individual genetic, immunological and bacteriological parameters.

The aim of the study – to establish average quantitative indicators of major periodontal pathogens in plaque smears of patients with stage I periodontitis.

Materials and Methods. Study and control groups of patients were formed in order to achieve the aim of the research. The study group included 19 persons with stage I periodontitis, while control group included 17 persons without clinically registered signs of periodontal tissue damage. The periodontitis stage was evaluated according to the recommendations of the American Academy of Periodontology proposed in 2017. The quantitative evaluation of target periodontal pathogens during normal conditions and during stage I periodontitis was performed using “Parodontoscreen” analysis, which was performed under laboratory conditions and included provision of polymerase-chain reaction to identify the number of opportunistic microorganisms in real time.

Results and Discussion. The analysis revealed the following average quantitative indicators of key periodontal pathogens in smears taken from the dental plaque among patients with I stage periodontitis: P. gingivalis – (5.4±0.3) Lg (GE/sample), T. forsythia – (5.1±0.2) Lg (GE/sample), P. intermedia – (5.0±0.5) Lg (GE/sample), T. denticola – (3.9±0.7) Lg (GE/sample). The total bacterial mass was (6.7±0.4) Lg (GE/sample).

Conclusions. Based on the data obtained, it can be stated that there is a statistically significant increase in the number of all tested microorganisms during stage I periodontitis compared to the indicators that were registered among the control group patients without clinically expressed signs of periodontal tissue damage (р<0,05). Further studies should aimed at the modification of existing periodontal treatment protocols by their individualization, taking into account the recorded changes in the microbiological profile.

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Published
2019-11-08
How to Cite
Goncharuk-Khomyn, M. Y., Melnychuk, N. I., Tsuperyak, S. S., Pohoretska, K. V., & Patskan, L. O. (2019). Microbiological profile quantitative changes evaluation among patients with stage I periodontitis . Clinical Dentistry, (3), 11-17. https://doi.org/10.11603/2311-9624.2019.3.10571
Section
Terapeutic stomatology