Activity of the humoral type of adaptive immune system in patients with chronic generalized periodontitis and type 2 diabetes

  • O. Yu. Balitska I. Horbachevsky Ternopil National Medical University
  • Yu. I. Bondarenko I. Horbachevsky Ternopil National Medical University
  • H. H. Habor I. Horbachevsky Ternopil National Medical University
Keywords: type 2 diabetes mellitus, chronic periodontitis, humoral immunity


Summary. Systemic changes in diabetes can cause local pathological processes, including those from the innate immune system and the adaptive humoral immune response, involved in the development of periodontitis. Therefore, at this stage of the study, the need arose for a deeper study of the mechanisms of development and course of chronic generalized periodontitis in the presence of diabetes mellitus.

The aim of the study – to elucidate the nature of the reaction of the humoral link of adaptive immunity in patients with chronic generalized periodontitis, type 2 diabetes mellitus and their combination.

Materials and Methods. A survey was conducted of 20 practically healthy individuals (control group), 36 patients with a clinically established diagnosis of type 2 diabetes mellitus (DM), 32 patients with a confirmed diagnosis of chronic generalized periodontitis (CGP), and 32 patients with combined diabetes and CGP (group 4). The content of the main classes of immunoglobulins IgA, IgG and IgM was determined according to G. Manchini, the level of circulating immune complexes (CEC) was determined according to the method of V. Gashkov in the modification of Yu. Grinevich.

Results and Discussions. The level of Ig A and Ig G in the experimental groups was significantly higher relative to the control group. The Ig M level in group 2 was statistically significantly lower by 1.2 times, and in groups 3 and 4 it was significantly higher by 1.7 and 1.2 times, respectively. The fact that type 2 diabetes complicates the course of periodontitis is also evidenced by the concentration of immune complexes. In this case, the lowest level of CEC was detected in the group of patients with CGP, and the highest – with a combination of pathologies.

Conclusion. In chronic generalized periodontitis in the presence of type 2 diabetes mellitus, the humoral link of the adaptive immune system undergoes significant changes, as evidenced by an increase in blood serum concentrations of class A, M, G immunoglobulins and circulating immune complexes.


Amassi, B.Y., & Dakheel, R.S. (2017). Oral hygiene practice of adult diabetic patients and their awareness about oral health problems related to diabetes. Journal of Dentistry and Oral Hygiene, 9 (2), 8-14.

Jebouri, M., & Hadeethi, H. (2014). Interactions of immunological parameters among patients with periodontal disease. International Scientific Conference and Annual Meeting of the Brazilian Association of Allergy and Immunology.

Taylor, G.W., Manz, M.C., & Borgnakke, W.S. (2004). Diabetes, periodontal diseases, dental caries, and tooth loss: A review of the literature. Compend. Contin. Educ. Dent., 25 (3), 179-184.

Linden, G.J., Linden, K., Yarnell, J., Evans, A., Kee, F., & Patterson, C.C. (2012). All-cause mortality and periodontitis in 60–70-year-old men: a prospective cohort study. Journal of Clinical Periodontology, 39, 940-946.

Antonyshyn, I.V., Maruschak, M.I., & Denefil, O.V. (2014). Stan peroksydnoho okysnennia lipidiv pry eksperymentalnomu diietindukovanomu alimentarnomu ozhyrinni [State of lipid peroxide oxidation in experimental diet-induced alimentary obesity]. Medychna khimiia ̶ Medical Chemistry, 16 (3), 61-65 [in Ukrainian].

Marushchak, M., Lisnianska, N., Krynytska, І., & Chornomydz, I. (2017). The mechanisms of apoptosis initiation in rats with chronic enterocolitis combined with streptozotocin-induced diabetes. Georgian Medical News, (270), 125-130.

Xia, C., Rao, X., & Zhong, J. (2017). Role of T Lymphocytes in Type 2 Diabetes and Diabetes-Associated Inflammation. Journal of Diabetes Research, ID 6494795.

Balitska, O.Yu., Bondarenko, Yu.I., & Habor, H.H. (2018). Aktyvnist protsesiv peroksydatsii lipidiv u khvorykh na khronichnyi heneralizovanyi parodontyt i tsukrovyi diabet 2 typu [Activity of lipid peroxidation processes in patients with chronic generalized periodontitis and type 2 diabetes]. Visnyk naukovykh doslidzhen – Bulletin of Scientific Research, 3, 98-101 [in Ukrainian].

Danylevskyy, N.F., & Borysenko, A.V. (2000). Zabolevaniya parodonta [Periodontal disease]. Kyiv: Zdorovia [in Russian].

Rodriguez-Segade, S., Camina, M.F., & Carnero, A. (1996). High serum IgA concentrations in patients with diabetes mellitus: agewise distribution and relation to chronic complications. Clin. Chem., 42 (7), 1064-1067.

Asare-Anane, H., Botchey, C.P.K., & Ofori, E.K. (2018). Altered immunoglobulins (A and G) in Ghanaian patients with type 2 diabetes. SAGE Open Medicine, Retrieved from:

Peleg, A.Y., Weerarathna, T., & McCarthy, J.S. (2007). Common infections in diabetes: pathogenesis, management and relationship to glycaemic control. Diabetes Metab. Res. Rev, 23, 3-13.

Kranthi, J., Sudhakar, K., Kulshrestha, R., Raju, P.K., Razdan, A., & Srinivasa, T. (2013). Comparison of the serum immunoglobulin IgM level in diabetic and nondiabetic patients with chronic periodontitis. J. Contemp. Dent. Pract, 14 (5), 814-818.

Ghurabi, B.H., Aldhaher, Z.A., Azawy, V.S.A., & Talal, S. (2015). Estimation of humoral immunological parameters among chronic periodontitis patients with type 2 diabetes mellitus. Journal of Natural Sciences Research, 5, 10, 127-131.

Awartani, F. (2010). Serum immunoglobulin levels in type 2 diabetes patients with chronic periodontitis. Journal of Contemporary Dental Practice, 11 (3), 1-8.

How to Cite
Balitska, O. Y., Bondarenko, Y. I., & Habor, H. H. (2019). Activity of the humoral type of adaptive immune system in patients with chronic generalized periodontitis and type 2 diabetes. Clinical Dentistry, (3), 62-67.
Experimental researches