The features of oral microbiocenosis in rats with periodontitis on the background of hyper- and hypothyroidism

  • V. V. Shcherba I. Horbachevsky Ternopil National Medical University
  • M. M. Korda I. Horbachevsky Ternopil National Medical University
Keywords: periodontitis, thyroid dysfunction, microbiocenosis

Abstract

Summary. The oral cavity is an ecological system that is inhabited by more than 700 species of microorganisms, organized into a biofilm. Phenomena of dysbiosis play an important role in the pathogenesis of periodontitis.

The aim of the study – to carry out a comparative analysis of the frequency of colonization by oral microorganisms in rats of the control group and in animals with periodontitis without comorbidities and against the background of hyper- and hypothyroidism.

Materials and Methods. The experiments were conducted on 48 outbred adult white rats male weighing 180-200 g. Material sampling for microbiological studies (from the surface of teeth on the edge of hard tissue and gums in the interdental spaces) was performed using a standard sterile tampon of the Sarstedit transport system (Germany). Identification of isolated pure cultures was carried out according to morphological, tinctorial, cultural, biochemical properties and signs of pathogenicity.

Results and Discussion. Comparing the species composition and the percentage of excretion of certain types of microorganisms in rats of the control group and animals with simulated periodontitis, the changes in the qualitative composition of microflora, manifested by the appearance of strains of Staph. aureus, E. coli, P. aeruginosa, and yeast-like fungi of the genus Candida albicans. Comparing the species composition and the percentage of excretion of individual microorganisms in rats with simulated periodontitis without concomitant pathology and against the background of thyroid dysfunction, there was no change in the species composition, however, a significantly higher frequency of colonization for strains of Staph. aureus and yeastlike fungi of the genus Candida albicans. At the same time, no significant changes were found between groups with periodontitis on the background of hyper- and hypothyroidism.

Conclusions. Dystrophic-inflammatory process in the rats’ periodontium on the background of thyroid dysfunction is accompanied by an increase in the species number of microorganisms, which is probably a factor in enhancing the inflammatory processes in the periodontium, changes in its functional and antigenic properties, as well as a modulation factor of local and systemic immune reactions.

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Published
2019-09-06
How to Cite
V.V. Shcherba, V. S., & M. M. Korda, M. M. K. (2019). The features of oral microbiocenosis in rats with periodontitis on the background of hyper- and hypothyroidism. Clinical Dentistry, (2), 65-71. https://doi.org/10.11603/2311-9624.2019.2.10400
Section
Experimental researches