ANTIOXIDANT PROTECTION IN WHITE RATS ON THE BACKGROUND OF DRINKING WATER USE WITH EXCESSIVE NITRATE CONCENTRATION
DOI:
https://doi.org/10.11603/ijmmr.2413-6077.2022.1.12924Keywords:
drinking water, nitrates, superoxide dismutase, catalase, ceruloplasminAbstract
Background. The problem of nitrate pollution in terms of its scale and consequences has reached a global level. Approximately one-third of groundwater bodies for which information is currently available exceed the maximum allowable concentrations of nitrates in drinking water.
Objectives. The aim of the research is to study the features of antioxidant protection in the body of white rats on the background of drinking water with excessive concentrations of nitrates for 30 days regarding the age.
Methods. The experiment was performed on 2 groups of outbred white female rats of different age (mature and immature), 30 animals each, divided into 5 subgroups, which differed in the quality of drinking water they consumed independently. In a 30-day study, nitrates were given to rats with drinking water at a dose of 50, 150, 250, 500 mg of sodium nitrate per liter. The animals of the control group drank water from the city water supply. The effect of nitrates on superoxide dismutase (SOD), catalase (CAT) and ceruloplasmin (CP) in the serum of rats was evaluated.
Results. The research has shown increased SOD, CAT and CP in blood serum on the background of drinking water with a nitrate concentration of 150 to 500 mg/l in the experimental groups of animals of both ages. According to the research, the most significant changes in parameters are caused by drinking water with nitrates at a concentration of 500 mg/l. More significant changes were in immature animals then the mature ones.
Conclusions. Excessive concentrations of nitrates in drinking water adversely affect the body of experimental animals causing activation of the studied parameters of the antioxidant system.
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